| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,353,218 | 0 | G | C | 54.5% | 3.1 / 11.8 | 12 | R115P (CGT→CCT) | yrpB | 2‑nitropropane dioxygenase |
| Reads supporting (aligned to +/- strand): ref base G (3/2); new base C (6/0); total (10/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGT > NC_002947/4353090‑4353362 | cctgaacTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCCCCGATTTCACGGCGCCCACGCCCGTGTCCAACCCTGCGCCTTTCGACGAGc > 4:65580/1‑146 (MQ=255) ctgaacTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGGGCCGCTTTGACGGCGCCCCCCCCGGGGTGCAACCCTGCGCCGTTGGCCGCGCagc > 7:138708/1‑147 (MQ=255) ttctGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTAACCGCGCCCACGCCGGTGTCCAACCCTGCGCCTTTAGCCGAGCAGAGcagcctgc > 8:144357/1‑148 (MQ=255) ttctGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTAATACAGCGAAGTGGGGCCCCGTTTCACCGCGCCCCAGCAGCGTTCCAAACATGCCCCTGTGCACGAGCAGACcagcct > 8:58671/1‑146 (MQ=255) tGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGCTTTCACCGCGCCCCCGCCGGGGTCCAACCCTGCGCCTTTGGCCGCGCCGAGCGGGCTGCTgg > 3:58635/1‑148 (MQ=255) caGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGGGTCCAAACCTGCGCCATTCGACGCGCAGAGCGGCCTGGTGGGCGAAg > 8:144765/1‑148 (MQ=255) gAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGCTTTCACGGCGCCCCCGCCGGGGTCCAACCCTGGGCCTTTCGCCGAGCAGAGCTGCCGGCTGGTCGCCGCGTTTCGCCCGGAGGTGGTGa > 7:2257/1‑148 (MQ=255) ccccccccccACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAg < 2:145948/141‑1 (MQ=255) gtgtCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCa > 6:136482/1‑148 (MQ=255) cAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGAcc > 8:159807/1‑148 (MQ=255) aaCCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGCCCTGCGGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCCCGACCg > 7:78848/1‑148 (MQ=255) aCCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGt < 3:160699/148‑1 (MQ=255) | CCTGAACTTCTTCTGCCACCAGCCGCCAGCGCCCGATGCCGAGCGCGATGCGCGCTGGAAGCAGGCACTTGAGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGT > NC_002947/4353090‑4353362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |