Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,004,715	A→C	N356T (AAC→ACC)	PP_4410 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,004,715	0	A	C	100.0%	69.4 / NA	22	N356T (AAC→ACC)	PP_4410	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/8);  total (14/8)

CAATGGATGATTGGCTGAATGCAGAGCGTGCTTATTTGAGGCATCAACCAGTTGAAAAATTAAAAGCACGCAGAATGGCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAAACCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGAAATTCACAAGCTTCGTACATTATAGCTGCGATGCCCCTCTTTCCCCGGAGCGCCCCCTATCACTATGTGAACTTTGCGAAAAACTAGGTTACAAGGAGTCGAA  >  NC_002947/5004580‑5004856                                                                                                                                        |                                                                                                                                              caATGGATGATTGGCTGAATGCAGAGCGTGCTTATTTGAGGCATCAACCAGTTGAAAAATTAAAAGCACGCAGAATGGCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGaa                                                                                                                                   >  4:77654/1‑148 (MQ=255)                tGAATGCAGAGCGTGCTTATTTGAGGCATCAACCAGTTGAAAAATTAAAAGCACGCAGAATGGCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAACTTTAAGTTCCCCt                                                                                                                     >  5:145559/1‑147 (MQ=255)                   aTGCAGAGCGTGCTTATTTGAGGCATCAACCAGTTGAAAAATTAAAAGCACGCAGAATGGCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAac                                                                                                                 >  8:110128/1‑148 (MQ=255)                     gCAGAGCGTGCTTATTTGAGGCATCAACCAGTTGAAAAATTAAAAGCACGCAGAATGGCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAacac                                                                                                               <  6:84893/148‑1 (MQ=255)                          gCGTGCTTATTTGAGGCATCAACCAGTTGAAAAATTAAAAGCACGCAGAATGGCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGc                                                                                                           >  3:80579/1‑147 (MQ=255)                              gCTTATTTGAGGCATCAACCAGTTGAAAAATTAAAAGCACGCAGAATGGCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGaaa                                                                                                       >  2:163273/1‑147 (MQ=255)                                  aTTTGAGGCATCAACCAGTTGAAAAATTAAAAGCACGCAGAATGGCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGAAATTca                                                                                                   >  2:93628/1‑147 (MQ=255)                                    ttGAGGCATCAACCAGTTGAAAAATTAAAAGCACGCAGAATGGCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGAAATTCACaa                                                                                                <  3:74312/148‑1 (MQ=255)                                           aTCAACCAGTTGAAAAATTAAAAGCACGCAGAATGGCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACACCTCAACACCTGCGAAATTCACAAGCTTCg                                                                                          >  2:55910/1‑147 (MQ=255)                                                      gAAAAATTAAAAGCACGCAGAATGGCAATCGGGAAAATCAATATATACTTAGTTATATATCGACCCCGTTGGATCTGTCCACCCCCCCTGTCGAAGGTTAAGTGCCCGGCAACACGTGCGACAGTCTCAAGCTTCGTACCTTATAGCt                                                                              >  6:138694/1‑148 (MQ=255)                                                       aaaaaTTAAAAGCACGCAGAATGGCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGAAATTCACAAGCTTCGTACATTATAGCt                                                                              <  2:28900/147‑1 (MQ=255)                                                         aaaTTAAAAGCACGCAGAATGGCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGAAATTAACAAGCTTCGTACATTATAGCTGCg                                                                           >  4:93727/1‑148 (MQ=255)                                                          aaTTAAAAGCACTCAGAATGGCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGAAATTCACAAGCTTCGTACATTATAGCTGCGa                                                                          <  5:148032/148‑1 (MQ=255)                                                            ttAAAAGCACGCAGAATGGCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGAAATTCACAAGCTTCGTACATTATAGCTGCGATg                                                                        >  2:178679/1‑148 (MQ=255)                                                                              gCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGAAATTca                                                                                                   >  6:33926/1‑103 (MQ=255)                                                                               cAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGAAATTcac                                                                                                  <  5:33926/103‑1 (MQ=255)                                                                                          aTCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCGCAACACCAGCGAAAATAACAACCTTCGTAAATTATAGCTGCGAAGCCCCTCTTTACCCGGACCCCCCCCAAtaa                                           >  4:86565/1‑145 (MQ=255)                                                                                                         tttatATATCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGAAATTCACAAGCTTCGTACATTATAGCTGCGATGCCCCTCTTTCCCCGGAGCGCCCCCTATCACTATGTGAACTTTg                             <  2:14641/146‑1 (MQ=255)                                                                                                                 tCTACCCCTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGAAATTCACAAGCTTCGTACATTATAGCTGGGATGCCCCTCTTTCCCCGGAGCGGCCCCTATCACTATGTGAACTTTGCGAAAAACTa                   >  6:82215/1‑148 (MQ=255)                                                                                                                      cccTTTGGATCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGAAATTCACAAGCTTCGTACATTATAGCTGCGATGCCCCTCTTTCCCCGGAGCGCCCCCTATCACTATGTGAACTTTGCGAAAAACTAg                  <  4:20435/144‑1 (MQ=255)                                                                                                                               tCTTTCAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGAAATTCACAAGCTTCGTACATTATAGCTGCGATGCCCCTCGTTCCCCGGAGCGCCCCCTATCACTATGTGAACTTTGCGAAAAACTAGGTTACAAGGAGt      >  6:122044/1‑147 (MQ=255)                                                                                                                                    cAAACCCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGAAATTCACAAGCTTCGTACATTATAGCTGCGATGCCCCTCTTTCCCCGGAGCGCCCCCTATCACTATGTGAACTTTGCGAAAAACTAGGTTACAAGGAGTCGaa  <  6:163095/146‑1 (MQ=255)                                                                                                                                        |                                                                                                                                              CAATGGATGATTGGCTGAATGCAGAGCGTGCTTATTTGAGGCATCAACCAGTTGAAAAATTAAAAGCACGCAGAATGGCAATCGGGAAAATCAATATATACTTATTTATATATCTACCCCTTTGGATCTTTCAAAACCCCCTGTCGAAGTTTAAGTACCCCTCAACACCTGCGAAATTCACAAGCTTCGTACATTATAGCTGCGATGCCCCTCTTTCCCCGGAGCGCCCCCTATCACTATGTGAACTTTGCGAAAAACTAGGTTACAAGGAGTCGAA  >  NC_002947/5004580‑5004856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑