Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	46.5 / NA	16	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/8);  total (8/8)

ATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTA  >  NC_002947/499064‑499328                                                                                                                                            |                                                                                                                              aTCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATgtc                                                                                                                       >  1:34855/1‑146 (MQ=255)        cccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAc                                                                                                                <  8:124245/148‑1 (MQ=255)                    gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGCACGggccttgccgg                                                                                                    >  5:40120/1‑146 (MQ=255)                           gCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCCAGCCCGCGCACCGGCCGGGCctgcggctag                                                                                             >  8:17545/1‑142 (MQ=255)                                 gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGCACGGGCCTGGCCGGCGGATCTCTAttg                                                                                       >  3:60675/1‑146 (MQ=255)                                 gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATg                                                                                        <  4:90201/147‑1 (MQ=255)                                      aaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCCTGCCCGCGAACGGGCCTGGCCTGGGGATCTCGATGGCTGAg                                                                                  >  7:91998/1‑148 (MQ=255)                                           accTCGCGGGCGCAGCGGGCGAAGTCCGCCATCCAGCACAAGCTGATCCGATTGCGACATACGGGCGCCCGAAACTTGCCCACCCCTAGGGGCGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGCCCTCTATGGCTGAGCCGGc                                                                             <  2:31542/146‑1 (MQ=255)                                                 cTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTc                                                                       <  7:26570/148‑1 (MQ=255)                                                              tGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCGGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGcc                                                            <  3:170345/146‑1 (MQ=255)                                                                        cATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAAGGGCCTGGCCTGCTGAGCTCGAATGCTGAGCCGGCCGCTTCGCGGGCATTCCCGCTCCCACAgg                                                >  7:31843/1‑148 (MQ=255)                                                                                  aaGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACCCGCCAGGCCTGCTGAAATCTATGGCTGACCCGGCCTCATAGCGGGCATGCCCGCTCCCACACGTTTAGTGGtt                                      >  2:5434/1‑148 (MQ=255)                                                                                    gCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATTCCCGCGAACGGGCCTGGCCTGCTGATCTCTATTGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTACCACAGGTTTCGTGGTTgg                                    >  8:88116/1‑148 (MQ=255)                                                                                                           gTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCGGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCg             <  4:158982/148‑1 (MQ=255)                                                                                                                cGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTTATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATc        <  6:83403/148‑1 (MQ=255)                                                                                                                      ttGCCGAAGCCTTTGGGGGAGCGGGCAGGCCGGCTACCGGGCCGGGCGGGCTGATCTCTTTGGCTGAGCCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTa  <  4:60675/148‑1 (MQ=37)                                                                                                                                            |                                                                                                                              ATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTA  >  NC_002947/499064‑499328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑