| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 92.6% | 171.1 / 7.2 | 54 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (4/0); new base C (28/22); total (32/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACA > NC_002947/1932493‑1932776 | gcggcgTCTGCGAAGTGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑c < 4:54895/147‑1 (MQ=255) ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCGACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑GTGCCC‑GCGCAggg > 4:95478/1‑149 (MQ=38) caCATTCGACAGCGGCATTGCAGGGCTCACCAAAATAGCAGTAACCGCTGGGGCCGCTTTGCGGCCCTGCGCGGGCACGCCCGCTCCTACAAAGGATATGCGTCCCCTGTCGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGcccc < 2:283982/148‑1 (MQ=38) tCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAAtt > 1:94233/1‑149 (MQ=255) cGACCGCGGCATTGCAGGTCTCCCGAAAATAGCAGTAAACGCGGGGGCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTg < 7:138473/149‑1 (MQ=38) gaactcGGCATTGCAGGTCTCACGAAAATAGAAGTAAACGCTGGGGCGGCTTTGCGGCCCTTCGCGGGCATGCCCGCTCCTACAAAGGTTATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTg < 4:352096/143‑1 (MQ=38) aGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCTCCTACAAAGGATATGCGTACCCTGTATGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCaaaattact > 1:396503/1‑142 (MQ=37) aGCGGCATTGCAGGTCTCACGAAAATACCAGTAACCGCGGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATAGGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGa < 3:95478/149‑1 (MQ=37) ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTa > 3:342132/1‑149 (MQ=255) tttGCTGTTCTAACTAAAATTGCAGAAACCGCTGGGGCCGCTTTGCGGCCGTTCGCGGGCAAGCCCGCTCCTACAATGTATTTGCGTCCCCTGTTGTCGGGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcgc < 2:143881/148‑1 (MQ=255) cAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAAAAAGGATATGCGTGCCCTGTAGGAGCGGGCGTTC‑CC‑GCGAAGGGCTGGAAGGCACCCCCCAATTGTCGCATACGCGGa > 8:414445/1‑148 (MQ=37) aGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGAt > 2:344038/1‑148 (MQ=255) gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg < 7:435947/149‑1 (MQ=255) tctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGc > 1:84522/1‑149 (MQ=255) tcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCGCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAAGTGTCGACTACGCGGATCAGCGa > 2:88981/1‑149 (MQ=255) gAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCAGCAAAAAAGGATATGCCTGCCCGGTAGGAGCCGGCTTTG‑CC‑CCGAACGGCGGGAACGCCCCCCCCAATTTTGGACTAAGCGGGTAAGCGCTcag > 2:164739/1‑147 (MQ=255) gAAAATAGCAGTAAACGCTGGGGCCGCTTTGAGGCCCTTCGCGGGCAACCCCGCTCCTACAAAGGAAATGCGTACCCTGTAGGAGCGGGC‑TTG‑CCGGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACAACGCGGATCAGCGATCCg > 1:368024/1‑149 (MQ=39) caaaaTACCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAACCCCCCTCCAACAAATGATAATCGTCCCCACTAGCACCGGGC‑ATGCCC‑TCGAAGGGCAGCAAGGAAACCCCAAATTATCGACAACGCGGATCAGCGaaacc > 8:17088/2‑145 (MQ=255) aaTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCa < 4:363891/149‑1 (MQ=255) aaTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCa < 1:344038/149‑1 (MQ=255) aGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGCCTACGCGGATCAGCGATCCGGCAggg > 5:241819/1‑149 (MQ=255) aGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCACCTACAAAGGATATGCGTGCCCGGTAGGAGCGGGCTTTC‑CC‑GCGAAAGGCTGCAAGGCAGCCCCAAAATTTCGCCAACGCGGATCAGCGATACCGCAggg > 2:362910/1‑149 (MQ=25) gTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGAAATGCGTACCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCCACTACCCGGATCAGCGATCCCGCATGGTGAt > 4:363653/1‑149 (MQ=37) aaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGtt > 1:61355/1‑149 (MQ=255) gCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGTAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAg < 3:438381/149‑1 (MQ=255) gCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAg < 2:94233/149‑1 (MQ=255) gCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAg < 4:342132/149‑1 (MQ=255) gCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCCAATTGTCGGCTACGCGGATCAGCGATCCGGCAGGGGGATGTTCAGCTc > 7:37753/1‑146 (MQ=38) gCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTc > 5:425011/1‑146 (MQ=255) ccGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATAAGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAg > 2:134685/1‑148 (MQ=255) gCTTTGCGGCCCTTCGCGGGCAAGCCCGCACCTACAAAGGATATGCGACCCCGGTAGGAGCGGGC‑ATGCCC‑GCGAAGGGCTGCCAGGCAGCCCCAAATTGTCGACTACCCGGATAAGCGATCAGGCAGTGTGATGTTCAGCTCCAGAAt > 8:83334/1‑149 (MQ=37) cTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc < 1:362910/149‑1 (MQ=255) tttGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCGGTAGGAGCGGGC‑GTGCCC‑GCGTAGGGCTGCAAGGCCGCCCCAAATTGTCGACTACGCGGATAAGCGATCCGGCAGGGTGATGTTGAGCGGCAGAATc > 4:97437/1‑148 (MQ=37) cccTTCGCGGGCAAGCCCCCTCCTAAAAAGGATATGCGTCCCCAGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTGAGCACCAGAATCGAGCAGCt > 8:87074/1‑149 (MQ=38) gcgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTg < 7:414445/149‑1 (MQ=255) cgGGAAAGCCCGCTCCTACAAAGGATATGCGTCCCCTTTCGGAGCGGGC‑TGGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGATTCGAGCAGCTGCCCTgg < 2:368024/149‑1 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 8:242663/1‑149 (MQ=255) cGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGGGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCt < 1:164739/149‑1 (MQ=255) gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGGTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTc > 7:67438/1‑149 (MQ=255) gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTc > 4:97541/1‑149 (MQ=255) gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTc > 2:169294/1‑149 (MQ=255) cAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc < 3:97541/149‑1 (MQ=255) cAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc < 1:169294/149‑1 (MQ=255) tGCGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGTAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc < 3:97437/148‑1 (MQ=255) cGTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt < 1:134685/149‑1 (MQ=255) gTCCCCTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg > 4:25330/1‑149 (MQ=255) ccTGTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTCTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGa > 3:317583/1‑149 (MQ=255) gTAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGt > 7:321462/1‑149 (MQ=255) tAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCTGATCAGCGATCCGGCAGGGTTATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCAATATGcac < 5:172259/131‑1 (MQ=38) tAGGAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCTGCAGGGTGATGTTCAGCTCCAGAATAGAGCAGCTGCCATGGTTTTAGAGCTCGATATGcac > 6:172259/1‑131 (MQ=38) ggAGGGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc < 3:82255/148‑1 (MQ=255) ggAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc < 2:84522/148‑1 (MQ=255) gAGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca > 1:142856/1‑148 (MQ=255) aGCGGGC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTcaca > 2:351427/1‑149 (MQ=255) gggC‑TTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc > 4:331809/1‑110 (MQ=255) | GCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACA > NC_002947/1932493‑1932776 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |