Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,845,705	+G	intergenic (‑72/+26)	PP_3394 ← / ← PP_3395	3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,845,703	1	.	G	96.5%	188.8 / ‑0.1	57	intergenic (‑70/+28)	PP_3394/PP_3395	3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (31/24);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGC  >  NC_002947/3845556‑3845844                                                                                                                                                       |                                                                                                                                              ggCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑c‑gc‑g                                                                                                                                              >  7:349125/1‑149 (MQ=255) ggCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑c‑gc                                                                                                                                                <  1:384861/148‑1 (MQ=255)  gctgctGTCGGCATGATCACTTGCAGGCTCTGTAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GC‑gg                                                                                                                                             <  6:463952/149‑1 (MQ=255)  gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTTATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GC‑gg                                                                                                                                             <  8:222569/149‑1 (MQ=255)  gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GC‑gg                                                                                                                                             <  7:352417/149‑1 (MQ=255)    tgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GC‑gggc                                                                                                                                           >  5:260238/1‑147 (MQ=255)         tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGAt                                                                                                                                        <  2:401935/148‑1 (MQ=255)         tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGAt                                                                                                                                        <  2:352071/148‑1 (MQ=255)           ggCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATcc                                                                                                                                      <  8:137103/148‑1 (MQ=255)           ggCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCa                                                                                                                                     <  4:82671/149‑1 (MQ=255)           ggCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCa                                                                                                                                     >  4:265771/1‑149 (MQ=255)              aTGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCg                                                                                                                                  >  5:442407/1‑149 (MQ=255)               tGATCAGTTGCAGGCTCTGCAAGCGATCGGGCAGGCGGACTTCATTGATTGTTATTGTTGTCTTCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGt                                                                                                                                 <  4:77560/149‑1 (MQ=255)                gATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACAAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGt                                                                                                                                 >  2:36302/1‑148 (MQ=255)                 aTCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCa                                                                                                                               <  5:176748/149‑1 (MQ=255)                    aCTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGtggctg                                                                                                                            <  3:395797/149‑6 (MQ=255)                      ttGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCAc                                                                                                                          >  7:245633/1‑149 (MQ=255)                         cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGAt                                                                                                                       <  8:245633/149‑1 (MQ=255)                          aGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATg                                                                                                                      >  6:213476/1‑149 (MQ=255)                           ggCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTGTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATgg                                                                                                                     <  7:111478/149‑1 (MQ=255)                             ctctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGAt                                                                                                                   <  6:442407/149‑1 (MQ=255)                             ctctGCAAGCCATCGCGCAGGCCGACTGCATTGATTGTTAGTGGTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGCTGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGAt                                                                                                                   <  8:90977/149‑1 (MQ=255)                                 gCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGc                                                                                                               >  6:423712/1‑149 (MQ=255)                                  cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTCTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGc                                                                                                               <  3:265771/148‑1 (MQ=255)                                                  ccGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGcc                                                                                              >  4:318232/1‑149 (MQ=255)                                                   cGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCg                                                                                             <  5:460335/149‑1 (MQ=255)                                                     aCTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTTATCCATCGTCAGCCACGATGTATCAGCCCGCGCCCTGCCAGGCCGGt                                                                                           <  7:127179/149‑1 (MQ=255)                                                      cttatttgatttTTATTGTTGTAATCGGGGTCGGTCTGTCTCAAGCGTGGCGCCACACTAAGAGCTGTATCAGGGGCATAAACCACGTGTTT‑C‑GCGGGCTTACCAATCGTCAGCCACGATGTATAAGCCCGCGCCCTGCCAGGCCGGt                                                                                           <  8:349125/143‑1 (MQ=255)                                                         caatgattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGt                                                                                           >  4:113597/4‑145 (MQ=255)                                                         caatgattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGt                                                                                           <  3:113597/142‑1 (MQ=255)                                                         cATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCAc                                                                                       >  8:75361/1‑149 (MQ=255)                                                           ttgattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgc                                                                                     <  5:423712/149‑1 (MQ=255)                                                            tgattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACg                                                                                      >  1:106922/1‑147 (MQ=255)                                                            tgattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACg                                                                                      >  7:409226/1‑147 (MQ=255)                                                              attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgcagc                                                                                  <  5:213476/149‑1 (MQ=255)                                                                tgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCaa                                                                                >  5:344806/1‑149 (MQ=255)                                                                tgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GC‑gggc                                                                                                                                           >  2:363264/1‑87 (MQ=255)                                                                tgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GC‑gggc                                                                                                                                           <  1:363264/89‑3 (MQ=255)                                                                        ttgtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtggt                                                                        >  1:356975/1‑149 (MQ=255)                                                                        ttgtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtggt                                                                        >  7:411525/1‑149 (MQ=255)                                                                                gcgGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAgg                                                                >  7:323919/1‑149 (MQ=255)                                                                                    tctctcTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgcc                                                            <  6:344806/149‑1 (MQ=255)                                                                                        tctgtctgACGCGTTGCGCCAGACTAAGAGCTGGAGGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTTATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccga                                                          <  1:40440/147‑1 (MQ=255)                                                                                        tctgtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagc                                                        >  5:196858/1‑149 (MQ=255)                                                                                        tctgtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagc                                                        >  1:436141/1‑149 (MQ=255)                                                                                               gAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGc                                                 >  2:299513/1‑149 (MQ=255)                                                                                                aaGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGc                                                 >  7:403028/1‑148 (MQ=255)                                                                                                        cgcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtg                                        >  1:457509/1‑149 (MQ=255)                                                                                                           cAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGc                                     <  6:196858/149‑1 (MQ=255)                                                                                                               cTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGt                                 >  3:95723/1‑149 (MQ=255)                                                                                                                tAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTc                                >  1:172202/1‑149 (MQ=255)                                                                                                                  agagCTGGATGACGGGCAGTAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTcgc                              >  5:226270/1‑149 (MQ=255)                                                                                                                    agCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGGCAGGCCGGTGCACGCAGCAACACGTGGTCAAGCAGGCGCCGAGCCGCCAGCGAGAGCGTGTGCCGGTCgcgc                            >  1:448832/1‑149 (MQ=255)                                                                                                                      cTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCAt                          >  8:347440/1‑149 (MQ=255)                                                                                                                         gATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTTAAACATCCACACCCACCAACGATCACACCCCCCCCAGACACCCCGGACCACCCAGAAACAAGAGCACAAGCAACCGCCCAGCCGACAGCGCCAGCCTGTGACGGTcgccactgca                       >  8:178535/1‑142 (MQ=255)                                                                                                                         gATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATg                         >  8:178529/1‑147 (MQ=255)                                                                                                                         gATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATg                         >  5:441419/1‑147 (MQ=255)                                                                                                                             aCGTGCATAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGTTGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGtgtg                                      <  8:82772/130‑1 (MQ=255)                                                                                                                             aCGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGtgtg                                      >  7:82772/1‑130 (MQ=255)                                                                                                                               gggCAGAAAAGACGTGTTT‑C‑GCGGGCTGATACATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGAc                 >  2:332695/1‑149 (MQ=255)                                                                                                                                    taaatGACGTGTTT‑CGGG‑GGTTTATCCATCGTCAGCAACTATGGATCAGCCCGCGCCCTGCCAGGCCTGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAtt            <  8:409226/148‑1 (MQ=255)                                                                                                                                    gAAAAGACGTGTTT‑C‑GCGGGCTGATCCAGCGTCAGCCACTATGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAtt            <  2:106922/149‑1 (MQ=255)                                                                                                                                       aaGACGTGTTTGG‑GG‑GGCTGATCCTTCGTCAGCCACTTTGTATAAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCAc         <  8:411525/149‑1 (MQ=255)                                                                                                                                          aCGTGTTT‑C‑GCGGGCTGATACATAGTGAGCCACCATGGATAAGCCCGCGCCCAGCCAGGCCCGTGGACGCAGCAACAGGTGGTCAAGCAGGCGCCCAGCCGCCAGCGAGAGCCTGTGGCGGGCGCGCCTGGAGATGAGGAATTCACCaa      >  2:458301/1‑149 (MQ=255)                                                                                                                                               ttt‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAgcgc  <  7:347440/148‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                              GGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGC  >  NC_002947/3845556‑3845844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑