Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,647,927	G→A	Y265Y (TAC→TAT)	oprB‑II ←	carbohydrate‑selective porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,647,927	0	G	A	100.0%	224.6 / NA	67	Y265Y (TAC→TAT)	oprB‑II	carbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/32);  total (35/32)

GGACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAGTAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTCG  >  NC_002947/1647786‑1648068                                                                                                                                              |                                                                                                                                               ggACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGtaatagtag                                                                                                                                          <  5:188465/148‑1 (MQ=255)     gCCGGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGc                                                                                                                                     <  4:370299/149‑1 (MQ=255)     gCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCgg                                                                                                                                      >  7:253518/1‑148 (MQ=255)       cAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCAc                                                                                                                                   <  2:241155/149‑1 (MQ=255)       cAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCAc                                                                                                                                   <  1:402926/149‑1 (MQ=255)           gAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGata                                                                                                                               >  8:154354/1‑149 (MQ=255)             ggTGACCTGCTGATGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATAtt                                                                                                                             >  2:345664/1‑149 (MQ=255)                gACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGACTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTccc                                                                                                                          >  7:164346/1‑149 (MQ=255)                   ctgctgctgGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCTCGATATTCCCCtt                                                                                                                       >  4:270928/1‑149 (MQ=255)                   ctgctgctgGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCtt                                                                                                                       >  8:146987/1‑149 (MQ=255)                     gctgctgGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCttt                                                                                                                      >  2:9149/1‑148 (MQ=255)                     gctgctgGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCttt                                                                                                                      >  7:417794/1‑148 (MQ=255)                      ctgctgGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCa                                                                                                                    <  6:304522/149‑1 (MQ=255)                      ctgctgGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCa                                                                                                                    <  8:56468/149‑1 (MQ=255)                         ctgGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGc                                                                                                                 >  3:88886/1‑149 (MQ=255)                            ggCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCtt                                                                                                              <  7:41470/149‑1 (MQ=255)                            ggCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCtt                                                                                                              <  8:253518/149‑1 (MQ=255)                             gcgcCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTg                                                                                                             <  4:88886/149‑1 (MQ=255)                              cgcCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTgg                                                                                                            <  5:154979/149‑1 (MQ=255)                                ccAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGAt                                                                                                          <  5:152192/149‑1 (MQ=255)                                 cAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATa                                                                                                         >  5:409399/1‑149 (MQ=255)                                 cAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTGGGATa                                                                                                         >  8:242230/1‑149 (MQ=255)                                   aTCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGat                                                                                                                                >  7:303864/1‑124 (MQ=255)                                   aTCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGat                                                                                                                                <  8:303864/124‑1 (MQ=255)                                     ccACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTg                                                                                                     >  1:199618/1‑149 (MQ=255)                                      cacaAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTgg                                                                                                    <  1:248081/149‑1 (MQ=255)                                       acaAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTggg                                                                                                   <  5:196417/149‑1 (MQ=255)                                       acaAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTAGATACGTggg                                                                                                   >  1:102166/1‑149 (MQ=255)                                             cGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCAt                                                                                             <  5:403522/149‑1 (MQ=255)                                                 cttgctCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCa                                                                                         <  1:9149/149‑1 (MQ=255)                                                     ctCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGtt                                                                                      <  1:230482/148‑1 (MQ=255)                                                     ctCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTc                                                                                     >  3:140221/1‑149 (MQ=255)                                                       cGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCg                                                                                    >  1:65989/1‑148 (MQ=255)                                                        gAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGaa                                                                                  <  7:146987/149‑1 (MQ=255)                                                          aCTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGACTTACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAAc                                                                                 <  8:164346/148‑1 (MQ=255)                                                              ctgcGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGGGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑Gc                                                                             <  2:299412/147‑1 (MQ=255)                                                                 cGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTa                                                                         <  7:154354/148‑1 (MQ=255)                                                                  ggTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTAcc                                                                       >  2:434925/1‑149 (MQ=255)                                                                      ggcggcGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATAc                                                                                           <  3:137224/126‑1 (MQ=255)                                                                      ggcggcGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATAc                                                                                           >  4:137224/1‑126 (MQ=255)                                                                       gcggcgCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCAcc                                                                  >  4:355069/1‑149 (MQ=255)                                                                         ggcgCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCt                                                                >  1:237929/1‑149 (MQ=255)                                                                           cgccgcTGAGGGAGGCCGGCTGACCGTTGCTGTCCTTGATAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTgg                                                              <  7:392083/149‑1 (MQ=255)                                                                               gcTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGtgc                                                          >  4:333678/1‑149 (MQ=255)                                                                                cTGAGGGCGGCCGACTGACCGTTGCAGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCATTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGtgcc                                                         >  5:109711/1‑149 (MQ=255)                                                                                   aGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGtgccgct                                                      >  5:399006/1‑149 (MQ=255)                                                                                     ggcggcCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGtgccgctgc                                                    >  6:218177/1‑149 (MQ=255)                                                                                     ggcggcCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGtgccgctgc                                                    <  1:345664/149‑1 (MQ=255)                                                                                        ggccggcTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGtgccgctgccgc                                                 >  8:276217/1‑149 (MQ=255)                                                                                         gccggcTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTgccgctgccgct                                                <  6:409399/149‑1 (MQ=255)                                                                                                gACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGtgccg                                                        >  3:405146/1‑134 (MQ=255)                                                                                                gACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGtgccg                                                        <  4:405146/134‑1 (MQ=255)                                                                                                        cTGTCATTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCAtt                                 >  5:455274/1‑149 (MQ=255)                                                                                                              ttGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGAAGAGTAACCCACCCTGTGTGACCCTGAGGCTGTGATTGAAAACATAGCCg                             >  1:151502/1‑148 (MQ=255)                                                                                                                 agaACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTc                        <  2:65989/149‑1 (MQ=255)                                                                                                                      aTCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGt                   <  3:355069/149‑1 (MQ=255)                                                                                                                           gtgCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTgg                >  8:190352/1‑147 (MQ=255)                                                                                                                             gCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAgg            >  5:268769/1‑149 (MQ=255)                                                                                                                              ccTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTCGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAgg            <  2:199618/148‑1 (MQ=255)                                                                                                                                ttGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGc                               <  6:16809/127‑1 (MQ=255)                                                                                                                                ttGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGc                               >  5:16809/1‑127 (MQ=255)                                                                                                                                   gCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCt       <  1:330177/148‑1 (MQ=255)                                                                                                                                    cATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGc     >  1:338835/1‑149 (MQ=255)                                                                                                                                        aCTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTAGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTCg  <  2:102166/148‑1 (MQ=255)                                                                                                                                          tGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTAcc                                                                       <  3:441782/77‑1 (MQ=255)                                                                                                                                          tGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTAcc                                                                       >  4:441782/1‑77 (MQ=255)                                                                                                                                          tGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTCg  >  3:94451/1‑146 (MQ=255)                                                                                                                                          tGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTCg  >  5:202316/1‑146 (MQ=255)                                                                                                                                              |                                                                                                                                               GGACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAGTAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACG‑GCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTCG  >  NC_002947/1647786‑1648068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑