| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 100.0% | 118.6 / NA | 40 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (19/21); total (19/21) | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 97.5% | 117.9 / NA | 40 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (18/21); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑CCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAT > NC_002947/1499340‑1499616 || gACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTAGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCttt > 1:19177/1‑149 (MQ=255) cGGGCCGCAGCTCGACACGTGTGAAGCCCGTGATGCGGGGATTGTGTGCCTGAACGAAGCCTTGGCAGCAGAGCAGTCCTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCGGGGAAACAGGCGCCGCGGTGCATGGCACCGGCttatc < 8:109831/149‑4 (MQ=21) ggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg < 2:274773/149‑1 (MQ=255) ggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg < 4:61743/149‑1 (MQ=255) ggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTAGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg < 2:19177/149‑1 (MQ=255) gCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCgg > 6:443774/1‑149 (MQ=255) cAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGACACCGGCAAAGCCGgtgcc < 2:144736/149‑3 (MQ=38) aGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGTCTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttc < 2:370634/149‑2 (MQ=255) ttCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgc < 5:443774/149‑4 (MQ=255) cTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCCGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAcg‑ccc < 6:139693/149‑2 (MQ=25) cGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTg < 8:379506/113‑1 (MQ=255) cGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTg > 7:379506/1‑113 (MQ=255) cGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTg < 1:305593/148‑1 (MQ=21) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCggg > 5:165286/1‑149 (MQ=21) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCggg > 4:367283/1‑149 (MQ=25) ggCACCACAGCATCTCTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTAc < 7:130263/147‑1 (MQ=18) ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACa < 3:77234/148‑1 (MQ=21) ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑c < 2:337764/149‑1 (MQ=21) cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCCGTGTTAGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑cc > 7:267128/1‑148 (MQ=25) gcagGACTTCGGCTGCCAAACGGTGGGAGCTGGCGTGCCCGCTAAACTGGCGCCGCGGTGCATGGCACGGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGccc < 5:247267/149‑1 (MQ=21) agGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGGGTTGCCGGTGTTCGCGGGCACGCCCGCGCCCACCGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCgg > 3:398910/1‑148 (MQ=21) cTGCGGGTGCCAAAGGGTGGGAGCGGGGGTGCCCGCTAAACAGGCGCCGCGGTGCATGGGACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGttttt < 5:244181/149‑1 (MQ=21) gcggCTGCAAAAGGGGGGTCGGGGGGGTGCCCGCTAAACTGGCGCCGCGGTGCTTGGCACGGGTTTTGCGGGTGTTCGGGGGCAGGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGtttttt < 6:46700/146‑1 (MQ=255) aGGGTGCCAATCGGTGGGAGCGGGGGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCAGAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGtttttt < 7:348578/147‑1 (MQ=21) ggCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTCCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTttgtt < 3:387382/149‑1 (MQ=21) aaaCGGTGGGAGCGGGCGTGGCCCCGAAAAAGGCGCCCCCGTGGATTGCAACCGCTTTTCCCGTGTTAGCGGGCACGCCCGCGCACACAGACAGATTTGCAATTAA‑CCGGCCAATAGCGACACTGACCGGCCCGTTTTTT‑TTGccagg > 2:9335/1‑145 (MQ=255) gAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 7:184676/149‑1 (MQ=25) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACt > 5:168959/1‑149 (MQ=37) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCGCGCCCGCTCCCCCAGCCTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGGGA‑CGGCCCGGTTTTTGTTGCCTCGCTAACGAATCAGGACTCCCt > 7:400973/1‑148 (MQ=25) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 2:370989/1‑148 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 2:426956/1‑147 (MQ=37) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 3:157354/1‑146 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 7:174470/1‑148 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 4:114263/1‑148 (MQ=37) aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCt > 6:237566/1‑120 (MQ=25) aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCt < 5:237566/120‑1 (MQ=25) gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 2:204640/1‑148 (MQ=37) gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCa < 1:9335/149‑1 (MQ=37) gccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 6:303349/1‑149 (MQ=37) ggTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt > 1:157710/1‑149 (MQ=38) || GACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑CCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAT > NC_002947/1499340‑1499616 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |