| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 90.3% | 76.4 / ‑4.5 | 31 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (14/14); minor base A (3/0); total (17/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 100.0% | 87.7 / NA | 31 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (17/14); total (17/14) | |||||||||||
CTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑CCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCG > NC_002947/1499362‑1499646 || cTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCCGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAcg‑ccc < 6:139693/149‑2 (MQ=25) cGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTg < 1:305593/148‑1 (MQ=21) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCggg > 5:165286/1‑149 (MQ=21) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCggg > 4:367283/1‑149 (MQ=25) ggCACCACAGCATCTCTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTAc < 7:130263/147‑1 (MQ=18) ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACa < 3:77234/148‑1 (MQ=21) ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑c < 2:337764/149‑1 (MQ=21) cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCCGTGTTAGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑cc > 7:267128/1‑148 (MQ=25) gcagGACTTCGGCTGCCAAACGGTGGGAGCTGGCGTGCCCGCTAAACTGGCGCCGCGGTGCATGGCACGGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGccc < 5:247267/149‑1 (MQ=21) agGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGGGTTGCCGGTGTTCGCGGGCACGCCCGCGCCCACCGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCgg > 3:398910/1‑148 (MQ=21) cTGCGGGTGCCAAAGGGTGGGAGCGGGGGTGCCCGCTAAACAGGCGCCGCGGTGCATGGGACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGttttt < 5:244181/149‑1 (MQ=21) gcggCTGCAAAAGGGGGGTCGGGGGGGTGCCCGCTAAACTGGCGCCGCGGTGCTTGGCACGGGTTTTGCGGGTGTTCGGGGGCAGGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGtttttt < 6:46700/146‑1 (MQ=255) aGGGTGCCAATCGGTGGGAGCGGGGGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCAGAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGtttttt < 7:348578/147‑1 (MQ=21) ggCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTCCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTttgtt < 3:387382/149‑1 (MQ=21) aaaCGGTGGGAGCGGGCGTGGCCCCGAAAAAGGCGCCCCCGTGGATTGCAACCGCTTTTCCCGTGTTAGCGGGCACGCCCGCGCACACAGACAGATTTGCAATTAA‑CCGGCCAATAGCGACACTGACCGGCCCGTTTTTT‑TTGccagg > 2:9335/1‑145 (MQ=255) gAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 7:184676/149‑1 (MQ=25) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACt > 5:168959/1‑149 (MQ=37) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCGCGCCCGCTCCCCCAGCCTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGGGA‑CGGCCCGGTTTTTGTTGCCTCGCTAACGAATCAGGACTCCCt > 7:400973/1‑148 (MQ=25) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 2:370989/1‑148 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 2:426956/1‑147 (MQ=37) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 3:157354/1‑146 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 4:114263/1‑148 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 7:174470/1‑148 (MQ=37) aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCt < 5:237566/120‑1 (MQ=25) aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCt > 6:237566/1‑120 (MQ=25) gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 2:204640/1‑148 (MQ=37) gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCa < 1:9335/149‑1 (MQ=37) gccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 6:303349/1‑149 (MQ=37) ggTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt > 1:157710/1‑149 (MQ=38) ccGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 4:335292/1‑121 (MQ=39) ccGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 3:335292/121‑1 (MQ=39) gtgttcgcGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa > 3:414486/8‑149 (MQ=255) tgttcgcGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAt < 8:174470/143‑1 (MQ=255) tcgcGGCCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAc < 3:40999/146‑1 (MQ=255) cGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcgcg < 4:414486/149‑1 (MQ=255) || CTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑CCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGA‑CGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCG > NC_002947/1499362‑1499646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |