Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	508,771	Δ1 bp	7.7%	coding (240/1890 nt)	estP ←	esterase EstP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	508,771	0	C	.	7.7%	197.0 / 10.8	65	coding (240/1890 nt)	estP	esterase EstP
Reads supporting (aligned to +/- strand):  ref base C (31/27);  new base . (2/3);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

GGGTTTCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAACA  >  NC_002947/508634‑508913                                                                                                                                           |                                                                                                                                                gggTTTCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAg                                                                                                                                     <  2:54381/149‑1 (MQ=255) gggTTTCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACC‑ACGGATGTCGACGGGTTAAGCTCGCCTGGCGCGACGC‑CCAg                                                                                                                                     <  1:51078/148‑1 (MQ=255)       ccTGGGGGGCTGACGGTCTCGGACGTTCTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAggcggc                                                                                                                                <  5:73043/148‑1 (MQ=255)        cTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGG‑GCGTCGCGCCAGGCGAcc                                                                                                                               <  3:79476/148‑1 (MQ=255)         tGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGcc                                                                                                                               >  5:78429/1‑147 (MQ=255)           gggggATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGcc                                                                                                                               <  6:78429/145‑1 (MQ=255)            ggggATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGCCGCCTGG‑GCGTCGCGCCAggcga                                                                                                                                 >  8:72200/1‑141 (MQ=255)            ggggATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGCCGCCTGG‑GCGTCGCGCCAggcga                                                                                                                                 >  8:279023/1‑141 (MQ=255)            ggggATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGCCGCCTGG‑GCGTCGCGCCAggcga                                                                                                                                 <  7:72200/142‑2 (MQ=255)            ggggATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGCCGCCTGG‑GCGTCGCGCCAggcga                                                                                                                                 <  7:279023/142‑2 (MQ=255)                  gACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGc‑c                                                                                                                                        <  5:34921/129‑1 (MQ=255)                  gACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGc‑c                                                                                                                                        >  6:34921/1‑129 (MQ=255)                  gACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAgg                                                                                                                        >  5:17706/1‑145 (MQ=255)                  gACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAgg                                                                                                                        <  6:17706/145‑1 (MQ=255)                     ggTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGa                                                                                                                          <  5:258161/140‑1 (MQ=255)                     ggTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGa                                                                                                                          >  6:258161/1‑140 (MQ=255)                        ctcGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGatcatc                                                                                                                  >  7:228408/1‑145 (MQ=255)                        ctcGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGatcatc                                                                                                                  <  8:228408/145‑1 (MQ=255)                           ggACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGaca                                                                                                           >  5:80720/1‑149 (MQ=255)                             aCGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACAcc                                                                                                         >  3:52149/1‑149 (MQ=255)                                       gATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCAAGCGGGCTGGAAACCTACGCATTTCGACGGTTTAAGTACCCATGGCGCGCCGC‑CCAGGCGGCCACCGAGGAACATCGCCGCCACCGGGGCGaat                                                                                                >  1:86449/1‑148 (MQ=255)                                        aTCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGtt                                                                                              <  6:80720/149‑1 (MQ=255)                                              tCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAgg                                                                                                                        >  7:133776/1‑117 (MQ=255)                                              tCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAgg                                                                                                                        <  8:133776/117‑1 (MQ=255)                                              tCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAg                                                                                                                         >  4:31268/1‑116 (MQ=255)                                              tCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAg                                                                                                                         <  3:31268/116‑1 (MQ=255)                                               ccAGGATCTGCTGGGTGCAGTACCCGCCGACTGCCCAGTTATAACC‑ATCGGGCTGCATACCTACGCATGTCGACGGCTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGAGAAGTTGCAGTCg                                                                                       >  8:241117/1‑149 (MQ=255)                                                cAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGgc                                                                                                                                                 <  3:95/91‑1 (MQ=255)                                                cAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGgc                                                                                                                                                 >  4:95/1‑91 (MQ=255)                                                 aGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGaa                                                                                                 <  6:273412/137‑1 (MQ=255)                                                 aGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGaa                                                                                                 >  5:273412/1‑137 (MQ=255)                                                   gATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGt                                                                                   >  5:49275/1‑149 (MQ=255)                                                         ctgGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGt                                                                             >  2:309802/1‑149 (MQ=255)                                                           gggTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTg                                                                           <  1:309802/149‑1 (MQ=255)                                                              tggtgTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATGCCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTg                                                                        <  2:78913/149‑1 (MQ=255)                                                                gtgtACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGa                                                                       >  3:5832/1‑148 (MQ=255)                                                                    aCCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAAcgc                                                                  >  2:34765/1‑149 (MQ=255)                                                                     cccGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTc                                                                                        <  1:47164/126‑1 (MQ=255)                                                                     cccGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTc                                                                                        >  2:47164/1‑126 (MQ=255)                                                                             cTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTgg                                                          <  6:49275/148‑1 (MQ=255)                                                                                 ccAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACg                                                                               <  2:51757/123‑1 (MQ=255)                                                                                 ccAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACg                                                                               >  1:51757/1‑123 (MQ=255)                                                                                        ttaCC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCcaac                                              <  1:34765/149‑1 (MQ=255)                                                                                           cc‑ATCGGGCTGGATAACTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGAGAAGTTGCCGTAGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCcaac                                              >  7:168999/1‑146 (MQ=255)                                                                                              aTCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTg                                                                           <  6:112715/115‑1 (MQ=255)                                                                                              aTCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTg                                                                           >  5:112715/1‑115 (MQ=255)                                                                                              aTCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCcaac                                              >  3:57491/1‑144 (MQ=255)                                                                                               tCGTGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATc                                        <  2:86449/149‑1 (MQ=255)                                                                                                  ggCTGTATAGCTAAGGATGTCGACTGGTTATGGTCGCATGTGGCGACGC‑GCAGGCGGCCTCCGAGGATCATCGGCGACAGCGGTGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCTCCAACAAGAtggtg                                     <  8:168999/149‑5 (MQ=255)                                                                                                  ggCTGGATACCTACGGATGTCGACGGGTTAAGGTCGACTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCgg                                      >  8:182679/1‑148 (MQ=255)                                                                                                            cTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCggg                                                                                                      >  7:9437/1‑74 (MQ=255)                                                                                                            cTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCggg                                                                                                      <  8:9437/74‑1 (MQ=255)                                                                                                            cTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCg                           >  7:169675/1‑149 (MQ=255)                                                                                                            cTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCg                           <  4:57491/149‑1 (MQ=255)                                                                                                             tACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCgg                          >  7:16938/1‑149 (MQ=255)                                                                                                             tACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCT‑GGGTACCGCCAACAAGATCGGGAAACTGcc                             >  6:287684/1‑145 (MQ=255)                                                                                                             tACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCT‑GGGTACCGCCAACAAGATCGGGAAACTGcc                             <  5:287684/145‑1 (MQ=255)                                                                                                                    gTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGCCGAAGTTCCCGTCGGAGTCACGGTTGGTGAAACGCGCGCCTGGGGTACAGCCAACAAGATCGGGAAACTGCCCGGCATCGGc                   >  6:278762/1‑149 (MQ=255)                                                                                                                      cGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCg                           >  2:230287/1‑139 (MQ=255)                                                                                                                      cGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCg                           <  1:230287/139‑1 (MQ=255)                                                                                                                       gACGGGTTAAGGTCGCCTGGATTGATGC‑CCAGTCGGCCACCGAGGATCATCGGCGACAACGGGGCTAAGTTGCCGTCTGCGTCACGGTTTGTGAAACGCGCGCGTGGGGTACCGCCAACAAGATCGGGTTACTGCCGGGCTTCGGCGa                 <  4:5832/148‑1 (MQ=255)                                                                                                                            gTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGt           <  8:169675/149‑1 (MQ=255)                                                                                                                            gTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGGCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCTAACAAGATCGGGAAACTGCCCGGCATCGGCGAg                <  4:41045/144‑1 (MQ=255)                                                                                                                            gTTAAGGTAGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCGAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGcgat           >  3:41045/1‑146 (MQ=255)                                                                                                                             ttAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACTCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTc          <  7:241117/149‑1 (MQ=255)                                                                                                                             ttAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTc          >  5:122427/1‑149 (MQ=255)                                                                                                                                     gCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAaca  >  6:104452/1‑149 (MQ=255)                                                                                                                                           |                                                                                                                                                GGGTTTCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACC‑ATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAACA  >  NC_002947/508634‑508913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑