| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 685,580 | Δ1 bp | 8.0% | coding (667/2400 nt) | cadA‑II ← | cadmium translocating P‑type ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 685,580 | 0 | C | . | 8.0% | 158.3 / 7.1 | 50 | coding (667/2400 nt) | cadA‑II | cadmium translocating P‑type ATPase |
| Reads supporting (aligned to +/- strand): ref base C (25/21); new base . (2/2); total (27/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
TGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTACCAG > NC_002947/685433‑685727 | tGGCGGGGCTTTCCAGGTACTTGCCCAGCAATTCGAGGGGAATCTCCCCGGCGGAGGCTTCGAAGTACCGGGGCGGGGCCATGCCGGCGGGATCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGccac < 2:170168/148‑1 (MQ=255)tGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGccac > 3:265912/1‑148 (MQ=255) gcgGCTTTCCAGGTACTTGCCCAGCAATACCACGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACcag > 6:239709/1‑147 (MQ=255) ggCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAgg > 6:128101/1‑149 (MQ=255) cAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAgg < 1:277723/142‑1 (MQ=255) cAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAgg > 2:277723/1‑142 (MQ=255) cAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGt > 4:177575/1‑149 (MQ=255) aGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGtt > 3:296224/1‑149 (MQ=255) ttGCCCAGCAATACCAGGGCAATCACAACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTgccg‑gc > 7:161748/1‑149 (MQ=255) gCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTgccg‑gcgc > 6:18090/1‑149 (MQ=255) gccAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTg < 8:129469/140‑1 (MQ=255) cccAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTg > 7:129469/1‑141 (MQ=255) aGCAATACCAGGGCGATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCA‑CAGCAGGTCCATGTTGccg‑gcgccggcg > 7:268348/1‑149 (MQ=255) aGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCA‑CAGCAGGTCCATGTTGccg‑gcgccggcg > 3:39580/1‑149 (MQ=255) cAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACgg > 1:174013/1‑149 (MQ=255) aGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCA‑CAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGcc < 4:39580/149‑1 (MQ=255) aGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCA‑CAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGcc < 8:268348/149‑1 (MQ=255) accacGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGc < 6:303001/149‑1 (MQ=255) cGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCa < 5:239709/149‑1 (MQ=255) ttCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGc > 2:94855/1‑149 (MQ=255) gAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCgg < 8:161748/148‑1 (MQ=255) aGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCa < 4:265912/149‑1 (MQ=255) aGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTcc < 3:279060/90‑1 (MQ=255) aGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTcc > 4:279060/1‑90 (MQ=255) ggTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCgaga < 3:177575/149‑1 (MQ=255) cggcggGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGgt > 4:216821/1‑149 (MQ=255) ggcggGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGCCCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGgt < 1:94855/149‑1 (MQ=255) aGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCca < 5:18090/149‑1 (MQ=255) gCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCcag < 3:216821/149‑1 (MQ=255) cTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCcagca < 2:174013/149‑1 (MQ=255) cTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCcag > 2:229615/1‑147 (MQ=255) tgtgCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCcagcag > 2:61430/1‑149 (MQ=255) gtgCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCcagcag > 6:183563/1‑148 (MQ=255) ccATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACt < 5:128101/149‑1 (MQ=255) ccATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACt < 1:61430/149‑1 (MQ=255) aTTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTgc > 3:211306/1‑149 (MQ=255) ggTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTgcgc < 1:311665/148‑1 (MQ=255) gTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGcc > 7:73310/1‑148 (MQ=255) tCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCgg > 7:151236/1‑95 (MQ=255) tCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCgg < 8:151236/95‑1 (MQ=255) cATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATc > 1:76455/1‑149 (MQ=255) gcgcTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCaa < 5:54855/149‑1 (MQ=255) ctgcTGGTGGCCCTGGGCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGc > 6:130712/3‑147 (MQ=255) tgcTGGTGGCCCTGGGCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGCAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAg > 6:243594/2‑149 (MQ=255) cccAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACg < 8:73310/148‑1 (MQ=255) ccAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGt > 4:35515/1‑149 (MQ=255) ccAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGt < 6:59451/149‑1 (MQ=255) ccAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGt > 1:226479/1‑149 (MQ=255) aGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTg > 1:261827/1‑149 (MQ=255) ggCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATc > 6:12460/1‑127 (MQ=255) ggCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATc < 5:12460/127‑1 (MQ=255) gCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTAc < 3:35515/149‑1 (MQ=255) accaGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGAGGGTAGCATCCAGTGCAAGCCGACCGGTTGTACCAg > 7:31683/1‑149 (MQ=255) ccaGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCCTCAAGTGCACGCAGATCGTTGGTACCAg > 4:193338/1‑148 (MQ=255) | TGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCG‑GCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGTACCAG > NC_002947/685433‑685727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |