Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,174,227 Δ1 bp 6.6% coding (105/756 nt) PP_1029 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,174,2270G. 6.6% 195.9 / 7.3 61coding (105/756 nt)PP_1029membrane protein
Reads supporting (aligned to +/- strand):  ref base G (29/28);  new base . (2/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01

GGCTAACCTTTGCCTGTCTTCCCAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAACCTGCTGCCGTCGCGCCGGGATGTGGTCCGCAGTGAATTGCTGCGGCTGCTGCCCTT  >  NC_002947/1174087‑1174373
                                                                                                                                            |                                                                                                                                                  
ggctTATCTTTGCCTGTCTTCCCATGAAGTGCTGCCTTGCTTGTCCAAGTGTCGTTTTCCGGCCTTGACTGGCTGCGGATTCTGTTCGCCATTGCGGCGGCCTACTTCGTCTTCGGCATCGCCGGTTTTGGTACTGCTCTGGTGgggg                                                                                                                                             <  4:262299/148‑1 (MQ=255)
 gctAACCTTTGCCTGTCTTCCCAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCggg                                                                                                                                            <  2:61388/148‑1 (MQ=255)
 gctAACCTTTGCCTGTCTTCCCAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCgg                                                                                                                                             <  8:172692/147‑1 (MQ=255)
 gctAACCTTGGCCTGGCTT‑CCAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGTCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGc                                                                                                                                          <  1:86120/149‑1 (MQ=255)
   tAACCTTTGCCTGTCTTCCCAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGcc                                                                                                                                         >  6:194203/1‑149 (MQ=255)
       cTTTGCCTGTCTTCCCAGGAACTGCTGCCATGCTTGCCCAACAGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCAGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTTCACTGGAGGCCGGGCCGGTg                                                                                                                                     >  2:153044/1‑149 (MQ=255)
       cTTTGCCTGTCTTCCCAGGAACTGCTGCCATGCTTGCACAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTg                                                                                                                                     >  7:34566/1‑149 (MQ=255)
          tGCCTGTCTTCCCAGCTTCTGCTGCCATGCTTGCCCAACTCTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGc                                                                                                                                    <  8:239096/147‑1 (MQ=255)
            ccTGTCTTCCCAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGAt                                                                                                                                >  4:45443/1‑149 (MQ=255)
             cTGTCTTCCCAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGAtt                                                                                                                               <  5:194203/149‑1 (MQ=255)
             cTGTCTTCCCAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGAtt                                                                                                                               <  3:274429/149‑1 (MQ=255)
             cTGTCTTCCCAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGAta                                                                                                                               >  5:145810/1‑148 (MQ=255)
              tGTCTTCCCAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGAtt                                                                                                                               >  7:254363/1‑148 (MQ=255)
               gTCTTCCCAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCa                                                                                                                             >  8:140397/1‑149 (MQ=255)
                tCTTCCCAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTc                                                                                                                              <  5:282315/147‑1 (MQ=255)
                  ttCCCAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCgg                                                                                                                                             <  3:155919/130‑1 (MQ=255)
                  ttCCCAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCgg                                                                                                                                             >  4:155919/1‑130 (MQ=255)
                     cccggAACTGCTTCGATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCAt                                                                                                                       <  3:205900/146‑1 (MQ=255)
                     ccAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCAt                                                                                                                       >  1:17282/1‑149 (MQ=255)
                      cAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATg                                                                                                                      >  7:229227/1‑149 (MQ=255)
                       aGGAACTGCTCCCATGCTTGCCCAACTGTCGTTTTCCGGCCGTGCCTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGGCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGc                                                                                                                     <  8:135028/149‑1 (MQ=255)
                           aCTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGtt                                                                                                                 >  8:1898/1‑149 (MQ=255)
                             tgctccCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCAATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTg                                                                                                                >  8:222378/1‑148 (MQ=255)
                               ctgcCATGCTTGCCCTAGTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCa                                                                                                             <  1:153044/149‑1 (MQ=255)
                                        ttGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCatcatc                                                                                                    >  4:124403/1‑149 (MQ=255)
                                                   tCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGGCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTTATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTg                                                                                           <  8:34566/147‑1 (MQ=255)
                                                    cGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTc                                                                                                              >  8:93356/1‑127 (MQ=255)
                                                    cGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTc                                                                                                              <  7:93356/127‑1 (MQ=255)
                                                     gTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACT‑GTGGCGGGGCCGGTGCTGATTCACt                                                                                                                           <  6:2449/112‑1 (MQ=255)
                                                     gTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACT‑GTGGCGGGGCCGGTGCTGATTCACt                                                                                                                           >  5:2449/1‑112 (MQ=255)
                                                     gTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACT‑GTGGCGGGGCCGGTGCTGATTCACt                                                                                                                           <  6:6989/112‑1 (MQ=255)
                                                     gTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCAGGTTTTGGTACTGCACT‑GTGGCGGGGCCGGTGCTGATTCACt                                                                                                                           >  5:6989/1‑112 (MQ=255)
                                                          ccGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTgctgct                                                                                   <  7:149404/148‑1 (MQ=255)
                                                           cGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACtggt                                                                                                                                                  >  5:254120/1‑84 (MQ=255)
                                                           cGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACtggt                                                                                                                                                  <  6:254120/84‑1 (MQ=255)
                                                                  gACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCg                                                                           <  7:140397/148‑1 (MQ=255)
                                                                       gCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGc                                                                       <  3:45443/147‑1 (MQ=255)
                                                                       gCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCcg                                                                     >  2:17546/1‑149 (MQ=255)
                                                                       gCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATACACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGcc                                                                      <  6:145810/148‑1 (MQ=255)
                                                                           ccGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCAACCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGtt                                                                 >  2:256481/1‑149 (MQ=255)
                                                                             gATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCg                                                               >  6:36292/1‑149 (MQ=255)
                                                                             gATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCg                                                               <  7:222378/149‑1 (MQ=255)
                                                                                  tGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGc                                                             <  8:244267/146‑1 (MQ=255)
                                                                                  tGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGc                                                             >  7:244267/1‑146 (MQ=255)
                                                                                    ttCGCCATTGCCGCTGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAAc                                                          <  8:254363/147‑1 (MQ=255)
                                                                                    ttCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAACct                                                        >  6:131328/1‑149 (MQ=255)
                                                                                        ccATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAACctgctg                                                    >  5:285046/1‑149 (MQ=255)
                                                                                                     cTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAACCTGCTGCCGTCGCGCCggg                                       <  7:1898/149‑1 (MQ=255)
                                                                                                      tACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTc                                                                            <  7:193494/111‑1 (MQ=255)
                                                                                                      tACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTc                                                                            >  8:193494/1‑111 (MQ=255)
                                                                                                        cATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAACCTGCTGCCGTCGCGCCGGGAt                                     <  1:17546/148‑1 (MQ=255)
                                                                                                        cATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGGGCTGCTGGATTTCGTGGCCGCGTTCGGCAACCTGCTGCCGTCGCGCCGGGAtg                                    <  3:15849/149‑1 (MQ=255)
                                                                                                             tCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAACCTGCTGCCGTCGCGCCGGGATGTGGTc                               >  2:79561/1‑149 (MQ=255)
                                                                                                               ttCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAACCTGCTGCCGTCGCGCCGGGATGTGGTCCg                             >  4:306893/1‑149 (MQ=255)
                                                                                                                    cATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAACCTGCTGCCGTCGCGCCGGGATGTGGTCCGCAGTg                        <  8:229227/149‑1 (MQ=255)
                                                                                                                    cATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATACCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCATTCGGCAACCTGCTGCCGTCGCGCCGGGATGTGGTCCGCAGt                         >  1:140475/1‑148 (MQ=255)
                                                                                                                          cGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAACCTGCTGCCGTCGCGCCGGGATGTGGTCCGCAGTGAATtgc                  >  1:103650/1‑149 (MQ=255)
                                                                                                                                  gTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAACCTGCTGCCGTCGCGCCGGGATGTGGTCCGCAGTGAATTGCTGCGgctg          <  3:124403/149‑1 (MQ=255)
                                                                                                                                   tACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTACGTGGCCGCGTTCGGCACCCTGCTGCCGTCGCGCCGGGATGTGGTCCGCAGTGAATTGCTGCGgctgc         >  4:204208/1‑149 (MQ=255)
                                                                                                                                     cTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAACCTGCTGCCGTCGCGCCGGGATGTGGTCCGCAGTGAATTGCTGCGGctgctg       <  2:17282/149‑1 (MQ=255)
                                                                                                                                        cACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAACCTGCTGCCGTCGCGCCGGGATGTGGTCCGCAGTGAATTGCTGCGGCTGCTGccc    >  4:142945/1‑149 (MQ=255)
                                                                                                                                         aCTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACTCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAACCTGCTGCCGTCGCGCCGGGATGTGGTCCGCAGTGAATTGCTGCGGCTGCTGCCCt   >  1:318026/1‑149 (MQ=255)
                                                                                                                                          cTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAACCTGCTGCCGTCGCGCCGGGATGTGGTCCGCAGTGAATTGCTGCGGCTGCTGCCCtt  >  6:311695/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
GGCTAACCTTTGCCTGTCTTCCCAGGAACTGCTGCCATGCTTGCCCAACTGTCGTTTTCCGGCCTTGACTGGCTGCCGATCCTGTTCGCCATTGCCGCGGCCTACATCGTCTTCGGCATCGCCGGTTTTGGTACTGCACTGGTGGCGGGGCCGGTGCTGATTCACTTCATGCCGTTGTCACGCATCATCCCGTTGCTGGTGCTGCTGGATTTCGTGGCCGCGTTCGGCAACCTGCTGCCGTCGCGCCGGGATGTGGTCCGCAGTGAATTGCTGCGGCTGCTGCCCTT  >  NC_002947/1174087‑1174373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑