Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,504,727 Δ1 bp 10.4% coding (514/1146 nt) yliI → aldose sugar dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,504,7270A.10.4% 142.9 / 11.5 48coding (514/1146 nt)yliIaldose sugar dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (18/25);  new base . (2/3);  total (20/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.56e-01

CCGGTACTGCAGTCGGCCGCGGGCAGCTGTCCGAAGACCGCGCCCGGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGCGTATTCTGCCGGACGGCGAAGTGCCCAAAGACAACCCCTTCGTCGGCAAGGACAACGTGCGCCCGGAGATCT  >  NC_002947/2504581‑2504868
                                                                                                                                                  |                                                                                                                                              
ccGGTACTGCAGTCGGCCGCGGGCAGCTGTCCGAAGACCGCGCCCGGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATc                                                                                                                                              <  1:72884/149‑1 (MQ=255)
ccGGTACTGCAGTCGGCCGCGGGCAGCTGTCCGAAGACCGCGCCCGGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATc                                                                                                                                              <  5:262203/149‑1 (MQ=255)
 cGGTACTGCAGTCGGCCGCGGGCAGCTGTCCGAAGACCGCGCCCGGCTGGAGAATTTCACCGTGATCTTCCGCGAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCg                                                                                                                                             <  6:288810/149‑1 (MQ=255)
            tCGGCCGCGGGCAGCTGTCCGAAGACCGCGCCCTGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCga‑g                                                                                                                                 <  5:220115/149‑1 (MQ=255)
            tCGGCCGCGGGCAGCTGTCCGAAGACCGCGCCCGGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGc                                                                                                                                     >  6:203921/1‑146 (MQ=255)
                 cgcgGGCAGCTGTCCGAAGACCGCGCCCGGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTC‑TCGCCCAGGGCGATGaacag                                                                                                                            <  6:286083/149‑2 (MQ=255)
                      gCAGCTGTCCGAAGACCGCGCCCGGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAAcaaccaac                                                                                                                       <  5:203921/149‑1 (MQ=255)
                             tCCGAAGACCGCGCCCGGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCTGCTCGCGGCTGGTGTTCGACCTCGAAGGCTACCTGTTCAACGCCCTGGGCGA‑GAACAACCAACGTCCCa                                                                                                                 >  8:276161/1‑148 (MQ=255)
                             tCCGAAGACCGCGCCCGGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTC‑TCGCCCAGGGCGATGaacag                                                                                                                            >  6:275805/1‑136 (MQ=255)
                             tCCGAAGACCGCGCCCGGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTC‑TCGCCCAGGGCGATGaacag                                                                                                                            <  5:275805/137‑2 (MQ=255)
                                gAAGACCGCGCCCGGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGc                                                                                                             >  2:109486/1‑149 (MQ=255)
                                      cgcgCCCGGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGa                                                                                                       >  3:97442/1‑149 (MQ=255)
                                        cgcCCGGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGAcc                                                                                                     >  1:139030/1‑149 (MQ=255)
                                            cGGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGTCCAGGTCCTggg                                                                                                 <  5:12615/149‑2 (MQ=255)
                                            cGGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGa                                                                                                 <  4:97442/149‑1 (MQ=255)
                                               cTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACaa                                                                                              <  8:45097/149‑1 (MQ=255)
                                                  gagaATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGtt                                                                                           >  7:85446/1‑149 (MQ=255)
                                                     aaTTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTC‑TCGCCCAGGGCGATGaacag                                                                                                                            >  6:236033/1‑112 (MQ=255)
                                                     aaTTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTC‑TCGCCCAGGGCGATGaacag                                                                                                                            <  5:236033/113‑2 (MQ=255)
                                                      aTTTCACCGTGATCTTCCGCCTGCAGCACAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTgcaa                                                                                       <  7:276161/149‑1 (MQ=255)
                                                      aTTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTgcaa                                                                                       <  8:85446/149‑1 (MQ=255)
                                                      aTTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTgcaa                                                                                       <  2:139030/149‑1 (MQ=255)
                                                         tCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTgcaagg                                                                                     <  8:244048/148‑1 (MQ=255)
                                                           aCCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTgcaaggc                                                                                    >  6:127265/1‑147 (MQ=255)
                                                            ccGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTgcaaggcaag                                                                                 <  4:157298/149‑1 (MQ=255)
                                                                       cGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGCGTa                                                                       <  8:286255/148‑1 (MQ=255)
                                                                        gCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GCACAACCAACGTACCCCTGCCCCGGACCTGGACAAGTTGCAAGGCAAGGTCGGGCGTAtt                                                                     >  1:79601/1‑149 (MQ=255)
                                                                          cagcagCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTgcaaggca                                                                                   <  2:153439/133‑1 (MQ=255)
                                                                          cagcagCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTgcaaggca                                                                                   >  1:153439/1‑133 (MQ=255)
                                                                               gcCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGCGTATTCTGCCg                                                               >  5:138220/1‑148 (MQ=255)
                                                                                 ccAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTg                                                                           >  5:121457/1‑134 (MQ=255)
                                                                                 ccAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTg                                                                           <  6:121457/134‑1 (MQ=255)
                                                                                  cAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGc                                                                          <  7:152423/134‑1 (MQ=255)
                                                                                  cAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGc                                                                          >  8:152423/1‑134 (MQ=255)
                                                                                       tGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGCGTATTCTGCCGGACGGCGaa                                                      >  3:626/1‑149 (MQ=255)
                                                                                         tCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTgcaaggcaag                                                                                 <  6:258079/120‑1 (MQ=255)
                                                                                         tCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTgcaaggcaag                                                                                 >  5:258079/1‑120 (MQ=255)
                                                                                         tCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGCGTATTCTGCCGGACGGCGAAGt                                                    <  4:626/149‑1 (MQ=255)
                                                                                               ggTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGCGTATTCTGCCGGACGGCGAAGTGCCCa                                               <  5:127265/148‑1 (MQ=255)
                                                                                                     cACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGt                                                                            >  3:210854/1‑113 (MQ=255)
                                                                                                     cACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGt                                                                            <  4:210854/113‑1 (MQ=255)
                                                                                                     cACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGCGTATTCTGCCGGACGGCGAAGTGCCCAAAGACaa                                        >  2:218900/1‑149 (MQ=255)
                                                                                                                gcgGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGCGTATTCTGCCGGACGGCGAAGTGCCCAAAGACAACCCCTtcgtc                              <  1:109486/148‑1 (MQ=255)
                                                                                                                          ttCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGCGTATTCTGCCGGACGGCGAAGTGCCCAAAGACAACCCCTTCGTCGGCAAGGACaa                   >  5:38320/1‑149 (MQ=255)
                                                                                                                              aCCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGCGTATTCTGCCGGACGGCGAAGTGcc                                                 <  6:45312/115‑1 (MQ=255)
                                                                                                                              aCCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGCGTATTCTGCCGGACGGCGAAGTGcc                                                 >  5:45312/1‑115 (MQ=255)
                                                                                                                                           ccTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGCGTATTCTGCCGGACGGCGAAGTGCCCAAAGACAACCCCTTCGTCGGCAAGGACAACGTGCGCCCGGAGATc   <  6:38320/148‑1 (MQ=255)
                                                                                                                                           ccTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGCGTATTCTGCCGGACGGCGAAGTGCCCAAAGACAACCCCTTCGTCGGCAAGGACAACGTGCGCCCGGAGATCt  <  1:218900/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CCGGTACTGCAGTCGGCCGCGGGCAGCTGTCCGAAGACCGCGCCCGGCTGGAGAATTTCACCGTGATCTTCCGCCAGCAGCCCAAGCTGTCGGTGGGTAACCACTTCGGCTCGCGGCTGGTGTTCGACCGCGACGGCTACCTGTTCATCGCCCTGGGCGA‑GAACAACCAACGTCCCACTGCCCAGGACCTGGACAAGTTGCAAGGCAAGGTCGTGCGTATTCTGCCGGACGGCGAAGTGCCCAAAGACAACCCCTTCGTCGGCAAGGACAACGTGCGCCCGGAGATCT  >  NC_002947/2504581‑2504868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑