| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 342,755 | Δ1 bp | 7.7% | coding (1272/1386 nt) | gabP‑I → | gamma‑aminobutyrate permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 342,755 | 0 | G | . | 7.7% | 163.6 / 7.4 | 52 | coding (1272/1386 nt) | gabP‑I | gamma‑aminobutyrate permease |
| Reads supporting (aligned to +/- strand): ref base G (27/21); new base . (2/2); total (29/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01 | |||||||||||
GTGATTGCCGTTTCGCAGCTGCGCATGCGCGCCCAGCGTGAAGCCCGCGGCGAGAAGATCACCTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGACAGCTGATCGGCGCTGGCTGAAATGAGAAGGCCGCGCCCTG > NC_002947/342609‑342903 | gTGATTGCCGTTTCGCAGCTGCGCATGCGCGCCCAGCGTGAAGCCCGCGGCGAGAAGATCACCTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAgg < 4:172853/148‑1 (MQ=255) tGATTGCCGTTTCGCAGCTGCGCATGCGCGCCCAGCGTGAAGCCCGCGGCGAGAAGATCACCTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGAt > 3:124976/1‑149 (MQ=255) tttCGCAGCTGCGCATGCGCGCCCAGCGTGAAGCCCGCGGCGAGAAGATCACCTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCg > 6:46832/1‑149 (MQ=255) gcgcATGCGCGCCCAGCGTGAAGCCCGCGGCGAGAAGATCACCTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGA‑GATCACCGTGCGGAGATCATTGc > 7:248612/1‑149 (MQ=255) gcgcATGCGCGCCCAGCGTGAAGCCCGCGGCGAGAAGATCACCTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGA‑GATCACCGTGCGGAGATCATTGc > 7:244304/1‑149 (MQ=255) cgcATGCGCGCCCAGCGTGAAGCCCGCGGCGAGAAGATCACCTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTg < 5:46832/148‑1 (MQ=255) cATGCGCGCCCAGCGTGAAGCCCGCGGCGAGAAGATCACCTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAgg > 7:69145/1‑125 (MQ=255) cATGCGCGCCCAGCGTGAAGCCCGCGGCGAGAAGATCACCTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAgg < 8:69145/125‑1 (MQ=255) cgcgcCCAGCGTGAAGCCCGCGGCGAGAAGATCACCTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTgc > 3:32542/1‑149 (MQ=255) gcCCAGCGTGAAGCCCGCGGCGAGAAGATCACCTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCgct > 6:20035/1‑149 (MQ=255) gCGTGAAGCCCGCGGCGAGAAGCTCACCTTCAAGATGTGGCTGTCCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGa < 4:97571/149‑1 (MQ=255) gCGTGAAGCCCGCGGCGAGAAGATCACCTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGa < 2:101164/149‑1 (MQ=255) gCGTGAAGCCCGCGGCGAGAAGATCACCTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGa < 7:215570/149‑1 (MQ=255) gCGTGAAGCCCGCGGCGAGAAGATCACCTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGa < 3:126505/149‑1 (MQ=255) cTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGGGGCTGGCTTGc > 8:282873/1‑149 (MQ=255) ttCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCt > 4:141784/1‑149 (MQ=255) cAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCt < 4:124976/147‑1 (MQ=255) aaGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCAGTCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATtg > 1:70474/1‑125 (MQ=255) aaGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATtg < 2:70474/124‑1 (MQ=255) aaGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTgg < 5:20035/148‑1 (MQ=255) aGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTg < 2:91080/149‑1 (MQ=255) gATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTg > 1:45946/1‑148 (MQ=255) gATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTg > 3:308104/1‑148 (MQ=255) cTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGA‑GATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGC‑GGTGCACCAGCAAg < 8:244304/149‑1 (MQ=255) cTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGA‑GATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGC‑GGTGCACCAGCAAg < 8:248612/149‑1 (MQ=255) ccGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTAtt < 4:134527/108‑1 (MQ=255) ccGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTAtt > 3:134527/1‑108 (MQ=255) cGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGAGTGAGGATCACCGTGGGGAGATCATTGCGACTGCGCTGCTGAGAATTGGTGTGGTGGCGGCTGGCTATCTGGTGCACC‑GCAAGCGCGAg > 2:236810/1‑149 (MQ=255) cTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGc > 1:50473/1‑149 (MQ=255) cTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGc > 1:45922/1‑149 (MQ=255) cTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGCGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCcgggc > 8:175633/1‑149 (MQ=255) tGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCcgggcg > 1:80372/1‑149 (MQ=255) tgCGACCATTTCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCcgggcggg < 1:236810/148‑1 (MQ=255) gACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGgcgggcgg > 7:73786/1‑149 (MQ=255) ccTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCggctggc > 2:185942/1‑101 (MQ=255) ccTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGa < 4:308104/148‑1 (MQ=255) cTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGa > 7:158057/1‑147 (MQ=255) cTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGACa < 2:45946/149‑1 (MQ=255) cA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGACAGCt < 8:73786/149‑1 (MQ=255) a‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGACAGCt > 4:239094/1‑148 (MQ=255) tCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTg < 6:70410/140‑1 (MQ=255) tCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTg > 5:70410/1‑140 (MQ=255) tggtggTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGACAGCTGATCGgc > 6:12268/1‑142 (MQ=255) tggtggTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGACAGCTGATCGgc < 5:12268/142‑1 (MQ=255) gtCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGACAGCTGATCGGCGCTGGCTGAAAt > 3:247107/1‑149 (MQ=255) gtCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGACAGCTGATCGGCGCTGGCTGAAAt < 4:32542/149‑1 (MQ=255) aTGGCGCTGCGTGAGGATCACCGTGCGGTGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGACAGCTGATCGGCGCTGGCTGAAAt > 1:176679/1‑146 (MQ=255) aTGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGACAGCTGATCGGCGCTGGCTGAAATgag < 4:247107/149‑1 (MQ=255) gcgcTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGACAGCTGATCGGCGCTGGCTGAAATGAGAAg < 3:239094/149‑1 (MQ=255) gTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCAcc < 2:287567/78‑1 (MQ=255) gTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCAcc > 1:287567/1‑78 (MQ=255) tGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATtg < 6:88266/47‑1 (MQ=255) tGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATtg > 5:88266/1‑47 (MQ=255) tGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGACAGCTGATCGGCGCTGGCTGAAATGAGAAGGCCGCGcc > 8:245683/1‑149 (MQ=255) tGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGACAGCTGATCGGCGCTGGCTGAAATGAGAAGGCCGCGcc > 5:281195/1‑149 (MQ=255) ggATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTACTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGACAGCTGATCGGCGCTGGCTGAAATGAGAAGGCCGCGCCCtg < 5:272532/149‑1 (MQ=255) | GTGATTGCCGTTTCGCAGCTGCGCATGCGCGCCCAGCGTGAAGCCCGCGGCGAGAAGATCACCTTCAAGATGTGGCTGTTCCCGGGCCTGACCTGGGCGACCATTGCCTTCA‑TCGTTGCCATTCTGGTGGTCATGGCGCTGCGTGAGGATCACCGTGCGGAGATCATTGCGACTGCGCTGCTGAGTATTGGTGTGGTGGCGGCTGGCTTGCTGGTGCACC‑GCAAGCGCGAGGCTGCCGGGCGGGCGGCGCTGGACAGCTGATCGGCGCTGGCTGAAATGAGAAGGCCGCGCCCTG > NC_002947/342609‑342903 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |