| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,842,904:1 | +G | 17.4% | intergenic (+48/‑249) | ccoP‑II → / → PP_4259 | cbb3‑type cytochrome c oxidase subunit/iron‑sulfur cluster‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,842,904 | 1 | . | G | 17.4% | 128.9 / 30.8 | 46 | intergenic (+48/‑249) | ccoP‑II/PP_4259 | cbb3‑type cytochrome c oxidase subunit/iron‑sulfur cluster‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (18/20); new base G (4/4); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
GCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCCATACTGCC > NC_002947/4842761‑4843051 | gCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACA‑AGGATCCTGTGTAg > 8:221990/1‑149 (MQ=255) cccAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAgg < 7:262711/149‑1 (MQ=255) cAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACA‑AGGATCCTGTGTAGGAg < 7:221990/149‑1 (MQ=255) aGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGc < 8:270113/149‑1 (MQ=255) aGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGc < 8:180430/149‑1 (MQ=255) aGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACA‑AGGATCCTGTGTAGGAGc < 1:83840/149‑1 (MQ=255) aacaacTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGCggg < 7:77100/149‑1 (MQ=255) aacTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGCGGGtt > 5:111387/1‑148 (MQ=255) aaCGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGaa > 3:98332/1‑147 (MQ=255) cAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAg < 8:270438/123‑1 (MQ=255) cAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAg > 7:270438/1‑123 (MQ=255) aaGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGTAGCGGGTTTACCCGCGAAGAGGCCgg < 2:219546/149‑1 (MQ=255) ggTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCt‑t < 6:10152/116‑1 (MQ=255) ggTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCt‑t > 5:10152/1‑116 (MQ=255) gCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTg > 6:146914/1‑149 (MQ=255) cggcTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGCggg < 6:281668/113‑1 (MQ=255) cggcTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGCggg > 5:281668/1‑113 (MQ=255) aCGTCTACAGCCTGTCGCACCAGGCTTAGCCGGCCATGGCTTAGTATGGCTGGCGTGGCCTCTTCGCGGGTAACCCCGC‑TCCTACACAGCATCCT‑TGTAGTTGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGc < 6:111387/148‑1 (MQ=37) aCGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCCC‑TCCAACAAAGGATACA‑TGTAGGACCGGGTTTACCCGCCAAGACGCCCGTAATGGCACAGCAtatatcc > 2:92450/1‑141 (MQ=37) gTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCGGCGTAC‑CAGGGTACG‑TGTAGGAGCGGGGTTTCCCGCGAAGAGGGCGGGTCTGGCACCGCATTtctggacgg > 7:127871/1‑143 (MQ=25) tACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑GCCTACCCAGGATCCA‑TGTAGGGGCGGGTTTACCCGCGCAGCCGCCGGTACGGGCACCGCATTTGTCGCAggg > 3:21287/1‑146 (MQ=37) ccTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TTTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGAc < 5:279516/149‑1 (MQ=255) ccTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAAACCGC‑GCCTACACAGGATCCT‑TGTAAGAGCCGGTTTACACGCGAAGAGGCCGGTAAAGGCACAGCATATCTCGCAGTCAAATgc > 3:51438/1‑147 (MQ=37) tCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCt < 4:98332/147‑1 (MQ=255) cGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACA‑AGGATCCTGTGTAGGAGCggg > 3:3994/1‑92 (MQ=21) cGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACA‑AGGATCCTGTGTAGGAGCggg < 4:3994/92‑1 (MQ=21) ccACCAGGCTGAGCCGGCCAAGGCTGAGTACGGCCGGCCTGGCCTCTTCGCGGGTAAACCCCC‑TCCTACACCGTATCCT‑TGTAGCAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGAt < 6:98696/148‑1 (MQ=37) aCCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATc > 7:212867/1‑148 (MQ=255) ggatctgCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAAtt < 7:241180/142‑1 (MQ=255) ccGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTTAAACCCC‑CCCGACACACGGTGCA‑TGGAAGGCCGGGGTTAACACCGAAGAGGCGGGGGCAGGGACAGGATTTGTGGCCGGCAAGGGACCAGGGTGAAtttttaaag > 8:241180/1‑141 (MQ=255) tGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCCCAGCATTTCTCGCAGGGaa > 7:148563/1‑114 (MQ=255) ggcaggcCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATAc < 8:212867/149‑1 (MQ=255) tctTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGa > 4:193777/1‑42 (MQ=255) ggcgGGTAAACCCGC‑TCATACTCAGTATCCT‑TGTTGTAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAAcgc < 1:92450/145‑1 (MQ=39) cgcgGTAAAACCCGT‑CCATACACAGTATCCT‑TGTTGGAGCGGTTTAACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAAcgc < 7:257731/146‑1 (MQ=255) tgTAAACCCTC‑TCATACACATCATCCT‑TTTAGTAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACATGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTaaa < 3:233436/148‑1 (MQ=39) gTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCg > 2:19625/1‑57 (MQ=255) ccGC‑TCCTACACCGTATCCT‑TGTCGGTGGGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCa < 8:39641/149‑1 (MQ=255) ccGC‑TCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCa < 5:146914/149‑1 (MQ=255) gC‑TCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGt > 5:83869/1‑149 (MQ=255) cTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCg > 2:70159/1‑148 (MQ=255) tACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAAt < 3:141861/87‑1 (MQ=255) tACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAAt > 4:141861/1‑87 (MQ=255) acaaGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAAc > 7:106231/4‑149 (MQ=255) aaGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGa > 7:296256/2‑127 (MQ=255) aaGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGa < 8:296256/126‑1 (MQ=255) gATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCCATAc > 5:62283/1‑149 (MQ=255) cT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATATCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCCATACTGcc < 1:70159/149‑1 (MQ=255) | GCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGC‑TCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCCATACTGCC > NC_002947/4842761‑4843051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |