Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,227,283:1 +T 9.5% coding (1776/2391 nt) PP_4606 ← ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,227,2831.T 9.5% 152.3 / 11.1 42coding (1776/2391 nt)PP_4606ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base . (20/18);  new base T (2/2);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

GACCGTCTCGCTTGTGACGGCGTCAAAGTTCGAGACCAGTACATTGCGCTTGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCGGCGTGAGGTACCCAGGCCTGGCCACTGATGTC  >  NC_002947/5227139‑5227429
                                                                                                                                                 |                                                                                                                                                  
gACCGTCTCGCTTGTGCCGGCGTCAAAGTTCGAGACCAGTACATTGCGCTTGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑gag                                                                                                                                                 <  2:290317/148‑1 (MQ=255)
gACCGTCTCGCTTGTGACGGCGTCAAAGTTCGAGACCAGTACATTGCGCTTGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑gag                                                                                                                                                 <  1:59670/148‑1 (MQ=255)
gACCGTCTCGCTTGTGACGGCGTCAAAGTTCGAGACCAGTACATTGCGCTTGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑gag                                                                                                                                                 <  5:102464/148‑1 (MQ=255)
gACCGTCTCGCTTGTGACGGCGTCAAAGTTCGAGACCAGTACATTGCGCTTGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑gag                                                                                                                                                 >  3:7596/1‑148 (MQ=255)
 aCCGTCTCGCTTGTGACGGCGTCAAAGTTCGAGACCAGTACATTGCGCTTGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑gagga                                                                                                                                               <  8:175429/149‑1 (MQ=255)
   cGTCTCGCTTGTGACGGCGTCAAAGTTCGAGACCAGTACATTGCGCTTGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGt                                                                                                                                             >  6:269390/1‑149 (MQ=255)
                aCGGCGTCAAAGTTCGAGACCAGTACATTGCGCTTGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACAtt                                                                                                                                >  1:84915/1‑149 (MQ=255)
                        aaaGTTCGAGACCAGTACATTGCGCTTGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCCCC‑GAGGAGTCGAGCACGACATTTCCGGTCa                                                                                                                        >  8:101246/1‑149 (MQ=255)
                                   ccAGTACATTGCGCTTGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATg                                                                                                             <  2:3035/149‑1 (MQ=255)
                                     aGTACATTGCGCTTGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGt                                                                                                           >  4:174071/1‑149 (MQ=255)
                                          aTTGCGCTTGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTTGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGt                                                                                                      >  5:94224/1‑149 (MQ=255)
                                           ttGCGCTTGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTC‑GGTGGCACCTgaggaggg                                                                                                                                            >  1:199865/1‑108 (MQ=255)
                                           ttGCGCTTGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTC‑GGTGGCACCTgaggaggg                                                                                                                                            <  2:199865/110‑3 (MQ=255)
                                                 ttGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCtt                                                                                               >  5:285234/1‑149 (MQ=255)
                                                    gTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGaa                                                                                            <  6:285234/149‑1 (MQ=255)
                                                            gAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCg                                                                                     <  4:9282/148‑1 (MQ=255)
                                                                aaTGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTa                                                                                <  5:269390/149‑1 (MQ=255)
                                                                     cAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGGTTGAACGACTCGCTGTAGCTGc                                                                           >  6:274607/1‑149 (MQ=255)
                                                                             cTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAAc                                                                   >  5:268774/1‑149 (MQ=255)
                                                                              tgtgATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACACGCTGTAGCTGCCATAGAAc                                                                   >  6:23453/1‑148 (MQ=255)
                                                                                tgATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGATCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACga                                                                 <  4:7596/148‑1 (MQ=255)
                                                                                  aTGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GATGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACgaga                                                               <  7:101246/148‑1 (MQ=255)
                                                                                            gCATGTTGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGgtcgtc                                                     <  6:94224/148‑1 (MQ=255)
                                                                                            gCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGgtcgtc                                                     >  5:210521/1‑148 (MQ=255)
                                                                                                 tCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACttt                                               <  6:210521/149‑1 (MQ=255)
                                                                                                         ggCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAAc                                         <  1:129941/147‑1 (MQ=255)
                                                                                                          gcgcCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGa                                       <  3:174071/148‑1 (MQ=255)
                                                                                                           cgcCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATa                                     >  4:107700/1‑149 (MQ=255)
                                                                                                              ccAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCg                                  <  6:268774/149‑1 (MQ=255)
                                                                                                                   tCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCGGCGt                              >  3:308309/1‑148 (MQ=255)
                                                                                                                     ccACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCg                                  >  7:80652/1‑142 (MQ=255)
                                                                                                                     ccACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCg                                  <  8:80652/142‑1 (MQ=255)
                                                                                                                     ccACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCGGCGTGAg                           >  7:185642/1‑149 (MQ=255)
                                                                                                                           cTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTg                                                                                  >  5:69597/1‑88 (MQ=255)
                                                                                                                           cTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTg                                                                                  <  6:69597/88‑1 (MQ=255)
                                                                                                                                cctcctCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCGGCGTGAGGTACCCAGGcc                >  6:299052/1‑149 (MQ=255)
                                                                                                                                    ctcAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGAc                                                  >  1:54365/1‑111 (MQ=255)
                                                                                                                                    ctcAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGAc                                                  <  2:54365/111‑1 (MQ=255)
                                                                                                                                    ctcAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCGGCGTGAGGTACCCAGGCCTgg             <  3:107700/148‑1 (MQ=255)
                                                                                                                                     ctcggTGGCACCTGAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCGGCGTGAg                           <  4:164772/131‑1 (MQ=255)
                                                                                                                                     ctcggTGGCACCTGAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCGGCGTGAg                           >  3:164772/4‑134 (MQ=255)
                                                                                                                                      cAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTg                                                           <  6:316197/100‑1 (MQ=255)
                                                                                                                                      cAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTg                                                           >  5:316197/1‑100 (MQ=255)
                                                                                                                                        ggTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCGGCGTGAGGTACCCAGGCCTGGCCACt        <  2:84915/149‑1 (MQ=255)
                                                                                                                                          tGCAACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGCGAATGAACGACACGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCGGCGAGAGGTACAAAGGCCTGGGCACTg       >  2:283258/1‑148 (MQ=255)
                                                                                                                                             cACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCGGCGTGAGGTACCCAGGCCTGGCCACTGATGt   <  5:23453/149‑1 (MQ=255)
                                                                                                                                              aCC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCGGCGTGAGGTACCCAGGCCTGGCCACTGATGTc  >  7:67500/1‑149 (MQ=255)
                                                                                                                                               cc‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCGGCGTGAg                           >  7:181351/1‑123 (MQ=255)
                                                                                                                                               cc‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCGGCGTGAg                           <  8:181351/123‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GACCGTCTCGCTTGTGACGGCGTCAAAGTTCGAGACCAGTACATTGCGCTTGGTGATATCGAACAATGCCAGGGTGCCTGTGATGCGGCCTGGCATGTCGAGCTTGGCGCCCAGCTCCCACGCCTTGCCCTCCTCAGGTGCCACC‑GAGGAGTCGAGCACGACATTGCCGGTCAGCGGCGCGATGGTCGAGTTGGGCTTGAACGACTCGCTGTAGCTGCCATAGAACGAGAGCTGGTCGTCGACTTTGTAAACGATACCGGCGTGAGGTACCCAGGCCTGGCCACTGATGTC  >  NC_002947/5227139‑5227429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑