Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,468,073:1 +A 5.7% coding (819/1047 nt) mltB ← membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,468,0731.A 5.7% 287.9 / 9.5 70coding (819/1047 nt)mltBmembrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base . (30/36);  new base A (2/2);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

GTGATCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGA  >  NC_002947/5467927‑5468218
                                                                                                                                                    |                                                                                                                                                 
gTGATCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑Tc                                                                                                                                                 >  4:27876/1‑149 (MQ=255)
   aTCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGAt                                                                                                                                              >  5:301691/1‑149 (MQ=255)
      gcgTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGcc                                                                                                                                           >  6:96804/1‑149 (MQ=255)
       cgTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCg                                                                                                                                          <  3:168741/149‑1 (MQ=255)
           gAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCggtg                                                                                                                                       <  3:27876/148‑1 (MQ=255)
            aaGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTTACCGGC‑TCGATGCCggtggt                                                                                                                                     <  1:12680/149‑1 (MQ=255)
            aaGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCggtggt                                                                                                                                     >  7:150442/1‑149 (MQ=255)
               ttcttcAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAg                                                                                                                                  <  8:82699/149‑1 (MQ=255)
               ttcttcAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAg                                                                                                                                  <  6:138805/149‑1 (MQ=255)
               ttcttcAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAg                                                                                                                                  >  5:164025/1‑149 (MQ=255)
               ttcttcAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAg                                                                                                                                  <  2:252408/149‑1 (MQ=255)
                 cttcAGGCCCATGCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACGAGCCAAGCGTACGCTACTCCCCT‑ACCGTTTTCACCGGC‑TCGATTCCGGTGGTCAgg                                                                                                                                 <  4:112678/148‑1 (MQ=255)
                     aGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGGCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCtt                                                                                                                             <  2:19818/148‑1 (MQ=255)
                     aGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTtc                                                                                                                            <  8:201150/149‑1 (MQ=255)
                               aGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGgcgcgc                                                                                                                  >  1:316157/1‑149 (MQ=255)
                                 tACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGCATCGA‑GCCggtgaaaac                                                                                                                                  >  1:63540/1‑126 (MQ=255)
                                 tACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGCATCGA‑GCCggtgaaaac                                                                                                                                  <  2:63540/131‑6 (MQ=255)
                                    tCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCAcc                                                                                                             >  7:82281/1‑149 (MQ=255)
                                              tctcGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAg                                                                                                   >  2:266096/1‑149 (MQ=255)
                                                  gCCCTCGAGACGGAATGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGgcgc                                                                                               <  5:96710/149‑1 (MQ=255)
                                                  gCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGgc                                                                                                 <  3:285641/147‑1 (MQ=255)
                                                    ccTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑Tc                                                                                                                                                 <  8:268297/97‑1 (MQ=255)
                                                    ccTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑Tc                                                                                                                                                 >  7:268297/1‑97 (MQ=255)
                                                    ccTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGa                                                                                             <  6:301691/149‑1 (MQ=255)
                                                     cTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGAc                                                                                            >  8:65431/1‑149 (MQ=255)
                                                        gagaCGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGa                                                                                         <  7:65431/149‑1 (MQ=255)
                                                        gagaCGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGa                                                                                         >  8:11685/1‑149 (MQ=255)
                                                        gagaCGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGAGCGCTCACCTTCCACCCAGGCGCGACTGa                                                                                         >  2:201961/1‑149 (MQ=255)
                                                         agaCGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGAc                                                                                        <  6:170064/149‑1 (MQ=255)
                                                          gaCGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCGCACCTTCCACCCAGGCGCGACTGAc                                                                                        >  8:261489/1‑148 (MQ=255)
                                                             ggAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCa                                                                                                    <  8:281403/133‑1 (MQ=255)
                                                             ggAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCa                                                                                                    >  7:281403/1‑133 (MQ=255)
                                                                    ggTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACAccc                                                                                 >  6:251379/1‑145 (MQ=255)
                                                                    ggTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACAccc                                                                                 <  5:251379/145‑1 (MQ=255)
                                                                     gTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCgtc                                                                            >  4:299096/1‑147 (MQ=255)
                                                                         ccGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCg                                                                                                                                          <  1:58862/83‑1 (MQ=255)
                                                                         ccGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCg                                                                                                                                          >  2:58862/1‑83 (MQ=255)
                                                                            gCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCAcc                                                                     <  1:266096/149‑1 (MQ=255)
                                                                              aGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCa                                                                   <  8:82281/149‑1 (MQ=255)
                                                                               gATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGCATCGA‑GCCggtgaaaa                                                                                                                                   <  6:91197/84‑5 (MQ=38)
                                                                               gATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGCATCGA‑GCCggtgaaaa                                                                                                                                   >  5:91197/1‑80 (MQ=38)
                                                                                    tcGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCggg                                                             <  6:124795/149‑3 (MQ=255)
                                                                                      gcgcAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTAACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTgg                                                            >  1:229588/1‑149 (MQ=255)
                                                                                        gcAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGgcgc                                                         <  2:229588/149‑1 (MQ=255)
                                                                                            cGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGa                                                     <  8:152830/149‑1 (MQ=255)
                                                                                                      aCCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGTCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGc                                           <  3:299096/149‑1 (MQ=255)
                                                                                                      aCCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGc                                           <  7:11685/149‑1 (MQ=255)
                                                                                                          aCCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACg                                       >  6:174896/1‑149 (MQ=255)
                                                                                                            cAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCt                                     >  3:99293/1‑149 (MQ=255)
                                                                                                               ccAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGcc                                  >  8:212049/1‑149 (MQ=255)
                                                                                                               ccAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGc                                   >  1:159671/1‑148 (MQ=255)
                                                                                                               ccAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGc                                   <  2:159671/148‑1 (MQ=255)
                                                                                                               ccAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGCTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGAGGCGCTTGAAGTAGCTCGCCACGCTGcc                                  >  5:325413/1‑149 (MQ=255)
                                                                                                                     gTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGc                            <  5:96804/149‑1 (MQ=255)
                                                                                                                      tACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCGGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCa                           >  5:325903/1‑149 (MQ=255)
                                                                                                                      tACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCa                           <  5:174896/149‑1 (MQ=255)
                                                                                                                        cGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATc                         <  7:261489/149‑1 (MQ=255)
                                                                                                                         gCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCg                        >  1:81085/1‑149 (MQ=255)
                                                                                                                          cAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGt                       <  7:212049/149‑1 (MQ=255)
                                                                                                                                cccT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAgg                   <  6:164025/147‑1 (MQ=255)
                                                                                                                                cccT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGgt                 >  3:108464/1‑149 (MQ=255)
                                                                                                                                 ccT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGgtt                >  4:163888/1‑149 (MQ=255)
                                                                                                                                 ccT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGgt                 >  4:223831/1‑148 (MQ=255)
                                                                                                                                 ccT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGgt                 <  3:223831/148‑1 (MQ=255)
                                                                                                                                     aCCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGgttgtt             >  8:16551/1‑149 (MQ=255)
                                                                                                                                       cGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTcc           <  2:81085/149‑1 (MQ=255)
                                                                                                                                              aCCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGatat     <  4:99293/148‑1 (MQ=255)
                                                                                                                                               ccGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTg   <  1:201961/149‑1 (MQ=255)
                                                                                                                                                cGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGa  <  3:163888/149‑1 (MQ=255)
                                                                                                                                                 ggC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGa  >  8:225353/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GTGATCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCT‑ACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGA  >  NC_002947/5467927‑5468218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑