| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,932,638:1 | +C | 100% | intergenic (‑105/+45) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 95.5% | 137.5 / 0.1 | 44 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (23/19); total (25/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCG > NC_002947/1932503‑1932767 | cGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCCAGCCCGCACCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGGTTGCCCGCGAAggg > 5:185536/1‑147 (MQ=255) gATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATTTGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAgg > 3:53433/1‑148 (MQ=255) tCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAACCCCCCGCCTAAAAACGGTTTGAGTCCCCGGTTGGACCC‑GGGGTTG‑CCCCGCAGGGCGGCAAgggcg > 5:214980/1‑146 (MQ=255) aTTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCaaa > 7:28155/1‑149 (MQ=255) aTTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCaaa > 3:111880/1‑149 (MQ=255) cGGCATGGCAGGTCTCACTAAAATCGCAGTAAACGCGGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACt < 2:78055/149‑1 (MQ=37) cATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCCACCCCGCTGCTAAAAAGGATATGGGGGCCCGGTAGGA‑GCGGGGGTTC‑CCGCGAAGGGCGGGAAGGCAGCCCCCAAATGTGGACTAcc > 6:32716/1‑148 (MQ=255) aTTCCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGGGGGCAAGCCCGCTCCTACAAAGCATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcgc < 7:121245/149‑1 (MQ=39) cAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCGAAAAAGGATATGCGTACCCTGTAGGA‑GG‑GGGGTTGCCCGCGCAGGGCTGCCAGGGAGCCCCAAATTGTGGACTACGCGGAt > 1:259523/1‑149 (MQ=37) ctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTATGCGGATCAGc > 1:582/1‑148 (MQ=255) tcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCCAGCCCGCTCCGACAAAGGATATGCGGCCCCTGTAGGA‑GC‑GGGCTTGCCCGCCAAGGGCTGCAAGGCAGCCCCAAATTTTCGGCTAAGCCGATCAGCgc > 3:303021/1‑148 (MQ=25) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc > 7:282213/1‑129 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 8:282213/129‑1 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTATGCGGATCAGCg < 2:582/146‑1 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGGCGACTACGCGGATCAGCGAtg > 4:34692/1‑148 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGAAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAgg > 4:258772/1‑114 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGAAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAgg < 3:258772/114‑1 (MQ=255) tAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg < 4:111880/149‑1 (MQ=255) aCGCTGGGGCCGCTTTGGGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGTATATGCGTCCCCTGTTGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc < 4:303021/149‑1 (MQ=38) ggCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGGGGCCCCTGGAGGC‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTGGACTACGCGGATCAGCGGTGCGGGAGGGGGATGTTCAGCTc > 5:183458/1‑147 (MQ=25) tttGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCg > 6:138687/1‑149 (MQ=255) cGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagc < 8:28155/148‑1 (MQ=255) cccTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCt > 3:207937/1‑149 (MQ=255) ccTTCGCGGGCAAGCCCGCTCCTACAAAGCATATGCGCCCCCTGTCGCC‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc < 7:53758/128‑1 (MQ=38) cTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGc > 5:237337/1‑149 (MQ=255) aaGCCCGCTCCTACAGGGGACGCATATCCTTTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGTCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGAt > 8:120069/1‑113 (MQ=14) aaGCCCGCTCCTACAGGGGACGCATATCCTTTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGTCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGAt < 7:120069/113‑1 (MQ=14) accccGCTCCTACATAGTATATGGGTCCCCTGTTGGC‑GG‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc < 2:259523/147‑1 (MQ=255) tCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGa > 4:297725/1‑149 (MQ=255) ctccAAAGTATTTGCTTCCCGTTTAGGT‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata < 6:185536/146‑1 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata < 4:207937/149‑1 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat < 3:297725/148‑1 (MQ=255) aCAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGa > 5:208513/1‑71 (MQ=255) aCAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGa < 6:208513/71‑1 (MQ=255) aCAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCa < 7:97513/139‑1 (MQ=255) aCAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCa > 8:97513/1‑139 (MQ=255) tatGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatcat > 1:294684/1‑149 (MQ=255) tatGCGTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatca > 5:31240/1‑148 (MQ=255) gTCCCCTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg > 5:75043/1‑149 (MQ=255) cccTGTAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCg < 6:75043/149‑1 (MQ=255) tAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa > 7:146670/1‑91 (MQ=255) tAGGA‑GC‑GGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa < 8:146670/91‑1 (MQ=255) gCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc < 7:138715/128‑1 (MQ=255) gCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGAAGATGCCCTGGTTTTCCAGATAGATATGCACatcatc > 8:138715/1‑128 (MQ=255) | CGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGA‑GC‑GGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCG > NC_002947/1932503‑1932767 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |