| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 278,742:1 | +A | 100% | intergenic (‑237/+77) | PP_0224 ← / ← sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 278,742 | 1 | . | A | 97.6% | 131.6 / ‑3.5 | 41 | intergenic (‑237/+77) | PP_0224/sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (20/20); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑AA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGC > NC_002947/278597‑278882 | gTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑aa > 6:220375/1‑148 (MQ=255) gCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑aaac > 8:223218/1‑145 (MQ=255) cAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGc < 5:294021/147‑1 (MQ=255) cAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGc < 7:223218/147‑1 (MQ=255) gCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCt < 3:134534/149‑1 (MQ=255) gATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTAGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTaca < 1:66364/149‑1 (MQ=255) aCAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGc < 4:38188/149‑1 (MQ=255) aCGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTc > 7:95055/1‑148 (MQ=255) gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACAAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCtgt < 7:127/147‑1 (MQ=255) cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTAcacaca > 7:67383/1‑129 (MQ=255) cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTAcacaca > 5:134434/1‑129 (MQ=255) cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTAcacaca < 6:134434/129‑1 (MQ=255) cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTAcacaca < 8:67383/129‑1 (MQ=255) tccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTc < 6:316613/114‑1 (MQ=255) tccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTc > 5:316613/1‑114 (MQ=255) cagccaCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCgg < 2:112010/141‑1 (MQ=255) ataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑ACCGCTCCTACACACATGCCACAAGATCTGTGGACTGTAGGAGCGGGTTTAc > 6:199392/1‑149 (MQ=255) taCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGTGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTAc < 2:287068/148‑1 (MQ=255) ccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACAATCCTCAAGAACTGTGCACGGTAGGAGCGGGTTTACCcgc > 4:33127/1‑149 (MQ=255) ataAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTAc > 6:211819/1‑134 (MQ=255) ataAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTAc < 5:211819/134‑1 (MQ=255) taAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCgg < 8:95055/148‑1 (MQ=255) aaaTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGaa > 1:103826/1‑148 (MQ=255) aaTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGaa > 4:92651/1‑147 (MQ=255) ctaactTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCg > 6:121466/1‑147 (MQ=255) taactTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTACTAAAAACATTCCTAAAAATCTGTGGACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGAACAGGTTTAATAGGAg > 5:241794/1‑149 (MQ=255) ttttGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑ACCGCTCCTACACACATGCCTCAAGATGTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGTAAAGGTTTACTCGGAGGCCg > 7:256687/1‑148 (MQ=255) tcctttctataGAAGGGGTTTTTGTTACAGAGGTGCCGGGCTCTTCGCGGTTAAA‑CCCGCTCCTACACACATGCCTCAATATCTGTGCACTTTAGGAGGGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTc < 2:273183/141‑1 (MQ=255) gAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTcc > 5:221718/1‑149 (MQ=255) aaTATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTccc > 5:279143/1‑149 (MQ=255) atatTTACACACGGGGTTTTTGTAACCCCGGCGCCGCGCTCCCCGCGCTT‑AACCCCGCTCCACCACCAACCCCTCATCTTCTGTCCAGTTTAGCCGGGGTTTAACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCg < 4:305929/149‑1 (MQ=255) ctatctaGAAGGGGGATTTGTTACAGCGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAACATCTGTCCACTGTCGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGa < 5:178986/143‑1 (MQ=255) taGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑ACCGCTCCTACACACATGCCTAAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTc > 1:273183/1‑139 (MQ=255) gAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCCCTCCTACACACCAGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTAATAGGCGGCCGCAGTCCCGAgagaaag > 6:178986/1‑142 (MQ=38) gggggATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTgcgc < 2:103826/149‑1 (MQ=255) gggATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCaa < 5:121466/149‑1 (MQ=255) ggATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTgcgc > 1:12163/1‑146 (MQ=255) gATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTATCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAg > 1:316769/1‑148 (MQ=255) agagGTGCCGGGCTCTTCGCGGGTAAA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGTGCAAGAACTGCcgcc > 6:322951/1‑147 (MQ=255) agGTGCCGGGCTCTTCGCGTGTAAA‑CCCGCTCCTACACCCATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTAc < 6:221718/149‑1 (MQ=255) gCTCTTCGCGGTTAAA‑CCCGCTCCTACACACATCCCTCAATATCTGTGCACTGTAGCAGCGGTTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGt < 5:183177/138‑1 (MQ=255) gCTCTTCGCGGGTAAA‑ACCCCTACAAAACACATGCCTCAAGATATTTGCACTGTAGGAGCGGGTTTAACCGCGAAAGGGCCGGAAAAGGTTTACTCGGAGGCCGCCGTCCCGAGGAAATTTCGCCAGAACTGCCGCGt > 6:183177/1‑138 (MQ=255) tcccgGGTAAA‑CCCCCCCCTACACACATGCCACACTCTCTGGCCCCTGTCGGAGCGGGTTAACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGgc < 8:256687/146‑1 (MQ=255) | GTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑AA‑CCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGC > NC_002947/278597‑278882 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |