Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	336,124:1	+T	100%	coding (209/237 nt)	PP_0278 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	336,124	1	.	T	100.0%	233.2 / NA	70	D70V (GAT→GTT)	PP_0278	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (32/38);  total (32/38)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335982‑336263                                                                                                                                                 |                                                                                                                                            ccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttacct                                                                                                                                       >  7:154501/1‑144 (MQ=255) ccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttacct                                                                                                                                       >  7:242209/1‑144 (MQ=255)       cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGCCCATAAATTCATGGGGGCAGCCGATTACATGTGCGa                                                                                                                                  >  6:164294/1‑149 (MQ=255)       cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGa                                                                                                                                  >  5:155237/1‑149 (MQ=255)           aTCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATg                                                                                                                              <  5:321836/149‑1 (MQ=255)               ccTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGcc                                                                                                                           >  2:298086/1‑148 (MQ=255)                   cgcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAg                                                                                                                        <  8:53553/147‑1 (MQ=255)                        tgagCTCGGCTTCCCCGTCTAGGCGGCTTAAGAGAATGTCTCTATCCTGATGGGCTACAT‑CATGCACCTGGTGCGCGAGGGCTATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCtg                                                                                                                  <  8:267435/147‑1 (MQ=255)                        agagCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCtga                                                                                                                 >  7:104230/1‑149 (MQ=255)                        agagCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCtga                                                                                                                 >  7:108679/1‑149 (MQ=255)                         gagCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCtgat                                                                                                                <  4:224055/149‑1 (MQ=255)                         gagCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCtgat                                                                                                                <  8:104230/149‑1 (MQ=255)                         gagCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCtgat                                                                                                                <  8:108679/149‑1 (MQ=255)                                gCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATAAAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACAATAAATTCGTGGGGGCAGCCGATTACATGTGCGAAATGGCCAAGGCGCTGATGAACGa                                                                                                          >  1:236967/1‑149 (MQ=255)                                  gTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGatat                                                                                                       >  4:189942/1‑149 (MQ=255)                                    ccccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                                      >  2:217763/1‑148 (MQ=255)                                     cccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCga                                                                                                    <  8:229280/149‑1 (MQ=255)                                      ccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                                      >  1:214006/1‑146 (MQ=255)                                                   cTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGTGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGACGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGt                                                                                       >  3:247483/1‑148 (MQ=255)                                                   cTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGt                                                                                       >  4:118802/1‑148 (MQ=255)                                                    tACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAgg                                                                                         <  7:292998/145‑1 (MQ=255)                                                    tACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAgg                                                                                         >  8:292998/1‑145 (MQ=255)                                                     aCGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAg                                                                                    >  3:69555/1‑149 (MQ=255)                                                      cGAGCATGTCTCGTTCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAg                                                                                    <  1:217763/148‑1 (MQ=255)                                                      cGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAgg                                                                                   >  6:158586/1‑149 (MQ=255)                                                      cGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAgg                                                                                   <  6:155237/149‑1 (MQ=255)                                                      cGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAg                                                                                    <  3:118802/148‑1 (MQ=255)                                                      cGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGAGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAgg                                                                                   >  3:186722/1‑149 (MQ=255)                                                         gCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGAt                                                                                                 <  3:183273/132‑1 (MQ=255)                                                         gCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGAt                                                                                                 >  4:183273/1‑132 (MQ=255)                                                           aTGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAt                                                                              <  1:298086/149‑1 (MQ=255)                                                            tGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAt                                                                              >  2:5008/1‑148 (MQ=255)                                                                tcGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCAAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAgg                                                                          >  4:212814/1‑148 (MQ=255)                                                                  gATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCa                                                                        <  3:175257/148‑1 (MQ=255)                                                                  gATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCa                                                                        <  8:242209/148‑1 (MQ=255)                                                                  gATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCAt                                                                       >  7:158121/1‑149 (MQ=255)                                                                  gATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAACGCGCATTAGGc                                                                         <  3:189942/147‑1 (MQ=255)                                                                   aTCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCAtt                                                                      <  8:154501/149‑1 (MQ=255)                                                                       tGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcg                                                                  >  7:185953/1‑149 (MQ=255)                                                                             gCTACAT‑CAAGCACCTGGTTCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCa                                                            <  8:292076/149‑1 (MQ=255)                                                                             gCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCa                                                            >  6:110290/1‑149 (MQ=255)                                                                              cTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAt                                                           >  4:303659/1‑149 (MQ=255)                                                                                 cAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAttt                                                         <  8:185953/148‑1 (MQ=255)                                                                                 cAT‑CAAGCACATGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGGAGCCGATTACCTGTGCGACATAGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAttt                                                         <  2:135197/148‑1 (MQ=255)                                                                                  aT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                                       >  5:108537/1‑149 (MQ=255)                                                                                   t‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                      <  4:69555/149‑1 (MQ=255)                                                                                     cAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCCGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTgg                                                     >  8:191/1‑149 (MQ=255)                                                                                            cTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGc                                              >  2:4960/1‑149 (MQ=255)                                                                                                  cgcgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGc                                         <  1:4960/148‑1 (MQ=255)                                                                                                         gCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGaa                                 <  8:46404/149‑1 (MQ=255)                                                                                                          cGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAAt                                <  5:164294/149‑1 (MQ=255)                                                                                                          cGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCCGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAAt                                <  7:191/149‑1 (MQ=255)                                                                                                           gATATGGATGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATg                               <  2:236967/149‑1 (MQ=255)                                                                                                           gATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATg                               >  3:107272/1‑149 (MQ=255)                                                                                                               tGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGc                            <  6:108537/148‑1 (MQ=255)                                                                                                                       gacCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagca                   <  5:158586/149‑1 (MQ=255)                                                                                                                       gacCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagca                   <  8:158121/149‑1 (MQ=255)                                                                                                                         ccATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagcagc                 <  3:303659/149‑1 (MQ=255)                                                                                                                          cATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGcc                >  1:128084/1‑149 (MQ=255)                                                                                                                             aaaTTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagca                   >  1:117271/1‑143 (MQ=255)                                                                                                                             aaaTTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagca                   <  2:117271/143‑1 (MQ=255)                                                                                                                              aaTTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCgg            >  2:187669/1‑149 (MQ=255)                                                                                                                              aaTTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGAGGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTTGCGAATGGGCTGCAAAGCAGCCCCgg            <  1:5008/149‑1 (MQ=255)                                                                                                                               aTTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCTCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGTAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGc           <  3:212814/149‑1 (MQ=255)                                                                                                                                   cTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGAt        <  1:187669/148‑1 (MQ=255)                                                                                                                                      ggggCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCa    <  5:110290/149‑1 (MQ=255)                                                                                                                                      ggggCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCATGCGGCGCGCATTGGGTGGCAAGCACGCCCGGCgcgttg     >  2:229141/1‑143 (MQ=255)                                                                                                                                        ggCAGCCGATTACCTGGGCGACATGGCCAAGGCGCTGATGAACGATATCGAGAGCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCaga  <  6:311629/149‑1 (MQ=255)                                                                                                                                           aGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCag   <  3:208698/145‑1 (MQ=255)                                                                                                                                           aGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCag   >  4:208698/1‑145 (MQ=255)                                                                                                                                            gCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGc                         >  5:105845/1‑122 (MQ=255)                                                                                                                                            gCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGc                         <  6:105845/122‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                            CCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACAT‑CAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335982‑336263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑