| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,845,703:1 | +G | 100% | intergenic (‑70/+28) | PP_3394 ← / ← PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,845,703 | 1 | . | G | 97.3% | 121.4 / ‑3.1 | 37 | intergenic (‑70/+28) | PP_3394/PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (24/12); total (25/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑C‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGC > NC_002947/3845556‑3845842 | ggCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTcg‑c < 6:38112/148‑1 (MQ=255) tCGGCATGATCAGTTGCAGGCTCTGGATGCCATCGAGCAGGACGACTTGATTGATTGTTATTGTTGTGATGGCGGTCTCTCTGTCTGAAGCGTTGAGCCAGACTAAGAGCTGGATGACGGGCAGATAAGACGTGTTTCG‑CGGGCTGAt < 4:284251/148‑1 (MQ=255) tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGAt < 6:33446/148‑1 (MQ=255) tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑C‑gggctga < 1:36295/147‑6 (MQ=255) tCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAg > 4:152091/1‑149 (MQ=255) gaaGGCTATTCAAGCCATAGCTCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGAGGTCTCTATGTCTGAAGCTTTGAGCCAGAATAAGATCTGGATGAAGGGCAGAAAAGACGTGTTTCGAG‑GGCTGATAAATCGTCAGACACGa > 5:106282/3‑149 (MQ=255) cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGAt < 2:45666/149‑1 (MQ=255) tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGAt > 1:101194/1‑148 (MQ=255) tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGAt > 5:293394/1‑148 (MQ=255) cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGcc > 1:81756/1‑149 (MQ=255) cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGc < 3:152091/148‑1 (MQ=255) aTCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGcc > 1:274979/1‑149 (MQ=255) gCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATACATCGTCAGCCACGATTGATAAGCCC‑GCGCCCTGCCAGGc > 1:155104/1‑149 (MQ=255) cTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGATGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTg > 5:319340/1‑149 (MQ=255) attgattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACg > 6:128350/1‑149 (MQ=255) attgattgTTATTGTTGTCATCGCGGTATATATGTCTGAAGCGTTGCGCCAGACTAAGAGATGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGATGATACATAGTCAGCCACGATGGATCAGCCC‑GCCCCCTGCCAGGCCGGTGAACg > 7:197572/1‑149 (MQ=255) ttgattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACg > 5:5427/1‑148 (MQ=255) attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGcc > 3:283074/1‑137 (MQ=255) tCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGGCGCCCTGCCAGGCCGGTGCACGCAGCAACAgg < 1:43322/143‑1 (MQ=255) tCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAgg > 2:43322/1‑142 (MQ=255) tCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAg > 3:275364/1‑147 (MQ=255) gcgGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATgg < 6:1663/96‑1 (MQ=255) gcgGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATgg > 5:1663/1‑96 (MQ=255) cgGGCTCTCTGTCTTAAGCGTTGCGCCACATTAAGAGCTGTTTCACGGGCATAAACGACGGGTTTCG‑CGGGCTCAGCCAGCGCCAGCAACGATGTTTCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGgc < 4:275364/149‑1 (MQ=255) tctgtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagc > 2:68015/1‑149 (MQ=255) gtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagccga > 3:100386/1‑149 (MQ=255) tctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGTCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagccga > 3:29105/1‑148 (MQ=255) gAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGc > 7:252437/1‑149 (MQ=255) cGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCgaga < 5:128350/149‑1 (MQ=255) gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATc < 5:262634/74‑1 (MQ=255) gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATc > 6:262634/1‑74 (MQ=255) aCTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCgg > 1:119462/1‑149 (MQ=255) tAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTc > 4:199483/1‑149 (MQ=255) tAAGAGCTGGATGACGGGCAGAAAAGACGTGTTACG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTc > 3:274275/1‑149 (MQ=255) gagCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTcgcg > 5:69695/1‑149 (MQ=255) cTGTATCACGGGCACAAACGAGGTGTTTCG‑CGGGCTCATCCATCGTCAGCCACTATGTATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCAt < 4:100386/149‑1 (MQ=255) gtttGACGGGCATAAAAGACGGGTTACG‑CGGGGTGATCCATCGTCAGCCACTATGTATCTGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGc < 4:274275/146‑1 (MQ=255) aaaGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCa < 1:68015/149‑1 (MQ=255) gtgtTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAgc > 7:222743/1‑149 (MQ=255) | GGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑C‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCC‑GCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGC > NC_002947/3845556‑3845842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |