| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,586,056:1 | +C | 100% | intergenic (+139/+76) | PP_4061 → / ← PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,586,056 | 1 | . | C | 93.8% | 46.8 / ‑3.0 | 16 | intergenic (+139/+76) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (6/9); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCC‑TCTCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑CGTGCCGGCCTTGAATGCGG‑CGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACC > NC_002947/4585913‑4586195 | cTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACCAGGG‑aggcc > 3:116000/1‑145 (MQ=39) ttAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACCAGGG‑A‑ccgtg > 4:299088/1‑145 (MQ=39) ggTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGGATTGATCGGGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACCAGGGCC‑CGTGCCGGc > 6:280174/1‑149 (MQ=38) aCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGa < 7:132391/149‑1 (MQ=39) cACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTAGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAAt < 6:273717/149‑1 (MQ=38) aCGGCACCGGCGTGAACTGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATg < 6:326725/149‑1 (MQ=38) aCGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATg < 3:299088/149‑1 (MQ=39) tGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCC‑TCTTCGCGGGTATACCCGCTCCTACCTGGG‑ACCGTGCCGGCCTTGAATGCGG‑CGCTGcccc < 2:50049/149‑1 (MQ=255) tGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGG‑CGCTGcccc < 7:112070/149‑1 (MQ=39) aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGG‑CGCTGCCCCTg < 2:191537/149‑1 (MQ=39) cTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGG‑CGCTGCCCCTGGTAGGAGCgg > 8:90987/1‑149 (MQ=38) gcgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCAGCTACGCGGGTAAACCCCCACCAACCAGGG‑ACCGTGCCGGCCTTGAATGCGG‑CGCGGCCCCGGGTTGGAGCggg > 2:77289/1‑149 (MQ=255) cgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGG‑CGCTGCCCCTGGTAGGAGCgg < 7:90987/146‑1 (MQ=38) gctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGG‑CGCTGCCCCTGGTAGGAGCGGGttt < 5:280174/149‑1 (MQ=38) gcgggTAAACCCGCTCCTACTAGGG‑ACCGTGCCGGCCTTGAATGCGGCCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCACCCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACg > 1:233484/4‑148 (MQ=38) gcgggTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGG‑CGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCa > 1:291946/4‑149 (MQ=255) ccGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGG‑CGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACtt > 3:51696/1‑149 (MQ=255) tACCAGGG‑ACCGTGCCGGCCTTGAATGCGG‑CGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCAcc > 4:254075/1‑149 (MQ=255) | CTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCC‑TCTCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑CGTGCCGGCCTTGAATGCGG‑CGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACC > NC_002947/4585913‑4586195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |