Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 14,234 A→G 7.5% E302G (GAA→GGA)  gyrB → DNA gyrase subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294714,2340AG 7.5% 150.8 / 2.6 53E302G (GAA→GGA) gyrBDNA gyrase subunit B
Reads supporting (aligned to +/- strand):  ref base A (26/23);  new base G (2/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TGAAGTCGCCCTGCAATGGAACGACAGCTTCAACGAAAACCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCAAGGACAAGCTGGTCTCCTCGGAGGTGAAAACCGCCGTG  >  NC_002947/14091‑14382
                                                                                                                                               |                                                                                                                                                    
tGAAGTCGCCCTGCAATGGAACGACAGCTTCAACGAAAACCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGcc                                                                                                                                                 >  8:95998/1‑149 (MQ=255)
 gAAGTCGCCCTGCAATGGAACGACAGCTTCAACGAAAACCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCt                                                                                                                                                >  6:292495/1‑149 (MQ=255)
   aGTCGCCCTGTAATGGAACGACAGCTTCAACGAAAACCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTgg                                                                                                                                              >  1:48034/1‑149 (MQ=255)
       gCCCTGCAATGGAACGACAGCTTCAATGAAAACCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGcc                                                                                                                                            >  1:149905/1‑147 (MQ=255)
        cccTGCAATGGAACGACAGCTTCAACGAAAACCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCaag                                                                                                                                         <  7:101857/149‑1 (MQ=255)
          cTGTAATGGAACGACAGCTTCAACGAAAACCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCaagaa                                                                                                                                       <  2:48034/149‑1 (MQ=255)
                  gAACGACAGCTTCAACGAAAACCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAgg                                                                                                                               <  4:73644/149‑1 (MQ=255)
                  gAACGACAGCTTCAACGAAAACCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAg                                                                                                                                <  8:239016/148‑1 (MQ=255)
                    aCGACAGCTTCAATGAAAACCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTg                                                                                                                             <  2:149905/149‑1 (MQ=255)
                    aCGACAGCTTCAACGAAAACCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTg                                                                                                                             <  1:96802/149‑1 (MQ=255)
                            ttCAACGAAAACCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTg                                                                                                                             >  1:251648/1‑141 (MQ=255)
                            ttCAACGAAAACCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTg                                                                                                                             <  2:251648/141‑1 (MQ=255)
                            ttCAACGAAAACCTACTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCAc                                                                                                                     >  6:320984/1‑149 (MQ=255)
                               aaCGAAAACCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTgg                                                                                                                  >  8:187116/1‑149 (MQ=255)
                                   aaaaCCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGcgac                                                                                                              <  7:95998/149‑1 (MQ=255)
                                        ctgctgTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGc                                                                                                         >  7:289363/1‑149 (MQ=255)
                                           ctgTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCg                                                                                                      <  6:104332/149‑1 (MQ=255)
                                            tgtgCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGt                                                                                                     >  4:49708/1‑149 (MQ=255)
                                             gtgCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGt                                                                                                     >  2:232602/1‑148 (MQ=255)
                                              tgCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGt                                                                                                     <  8:289363/147‑1 (MQ=255)
                                                cTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACGAGGTGGCAACCACTGGCGACGACGCCCGTGaa                                                                                                  <  5:40797/148‑1 (MQ=255)
                                                      caacaaTATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGa                                                                                           >  4:107403/1‑149 (MQ=255)
                                                        acaaTATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGaa                                                                                                                                                     <  4:180085/89‑1 (MQ=255)
                                                        acaaTATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGaa                                                                                                                                                     >  3:180085/1‑89 (MQ=255)
                                                                 cGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAg                                                                                                 >  8:14392/1‑132 (MQ=255)
                                                                 cGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAg                                                                                                 <  7:14392/132‑1 (MQ=255)
                                                                                gTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCg                                                                 <  7:187116/149‑1 (MQ=255)
                                                                                    ccACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGAcc                                                             <  3:49708/149‑1 (MQ=255)
                                                                                    ccACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGAcc                                                             <  3:137485/149‑1 (MQ=255)
                                                                                        cTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGaa                                                         <  4:302710/149‑1 (MQ=255)
                                                                                           gtggGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGtt                                                      <  5:320984/149‑1 (MQ=255)
                                                                                                  tCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGcc                                                                                                        <  4:17630/92‑1 (MQ=255)
                                                                                                  tCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGcc                                                                                                        >  3:17630/1‑92 (MQ=255)
                                                                                                     gTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCATGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCg                                               >  6:16586/1‑146 (MQ=255)
                                                                                                          tCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGGAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGccc                                         >  7:211439/1‑147 (MQ=255)
                                                                                                          tCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGGAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGAcc                                         >  1:4580/1‑147 (MQ=255)
                                                                                                          tCGATGACCCTTATCCTTAACAGCTACATCGAGCAGGGAGGCCTGGCCAATAAGAAGAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCaa                                       <  8:211439/149‑1 (MQ=255)
                                                                                                            gCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGGAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCaa                                       <  2:4580/147‑1 (MQ=255)
                                                                                                            gCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGTCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCAAg                                      <  5:16586/148‑1 (MQ=255)
                                                                                                                  ccGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGaa                                                         <  1:207269/123‑1 (MQ=255)
                                                                                                                  ccGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGaa                                                         >  2:207269/1‑123 (MQ=255)
                                                                                                                          ttAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGa                                                                                     >  6:156449/1‑87 (MQ=255)
                                                                                                                          ttAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGa                                                                                     <  5:156449/87‑1 (MQ=255)
                                                                                                                          ttAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAg                                                        <  4:281299/116‑1 (MQ=255)
                                                                                                                          ttAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAg                                                        >  3:281299/1‑116 (MQ=255)
                                                                                                                          ttAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCAAGGACAAGCTGGtctc                        >  8:242434/1‑148 (MQ=255)
                                                                                                                           tAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCAAGGACAAGCTGGTctcct                      >  3:254553/1‑149 (MQ=255)
                                                                                                                            aaCAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCAAGGACAAGCTGGTctcctc                     >  8:42958/1‑149 (MQ=255)
                                                                                                                                 cTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGTCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCAAGGACAAGCTGGTCTCCTCGGAgg                <  5:292495/149‑1 (MQ=255)
                                                                                                                                    cATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCAAGGACAAGCTGGTCTCCTCGGAGGTg              >  5:53235/1‑148 (MQ=255)
                                                                                                                                     aTCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCAAGGACAAGCTGGTCTCCTCGGAGGTGa             >  6:261010/1‑148 (MQ=255)
                                                                                                                                             ggAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCAAGGACAAGCTGGTCTCCTCGGAGGTGAAAAccgccg    >  2:204896/1‑149 (MQ=255)
                                                                                                                                              gAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGTGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCAAGGACAAGCTGGTCTCCTCGGAGGTGAAAACCGCCGt   >  4:9768/1‑149 (MQ=255)
                                                                                                                                              gAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCAAGGACAAGCTGGTCTCCTCGGAGGTGAAAACCGCCGt   >  8:262513/1‑149 (MQ=255)
                                                                                                                                              gAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCAAGGACAAGCTGGTCTCCTCGGAGGTGAAAACCGCCGt   >  1:13934/1‑149 (MQ=255)
                                                                                                                                               aaGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCAAGGACAAGCTGGTCTCCTCGGAGGTGAAAACCGCCGTg  <  7:242434/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TGAAGTCGCCCTGCAATGGAACGACAGCTTCAACGAAAACCTGCTGTGCTTTACCAACAATATTCCGCAGCGTGATGGCGGTACCCACCTGGTGGGTTTCCGTTCCTCGCTGACCCGTAGCCTTAACAGCTACATCGAGCAGGAAGGCCTGGCCAAGAAGAACAAGGTGGCAACCACTGGCGACGACGCCCGTGAAGGCCTGACCGCGATCATCTCGGTGAAGGTACCGGACCCGAAGTTCAGCTCGCAGACCAAGGACAAGCTGGTCTCCTCGGAGGTGAAAACCGCCGTG  >  NC_002947/14091‑14382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑