| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,664,878 | T→A | 9.5% | Y4* (TAT→TAA) | nuoI → | NADH‑quinone oxidoreductase subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,664,878 | 0 | T | A | 9.5% | 115.4 / 3.8 | 42 | Y4* (TAT→TAA) | nuoI | NADH‑quinone oxidoreductase subunit I |
| Reads supporting (aligned to +/- strand): ref base T (21/17); new base A (2/2); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01 | |||||||||||
CCCACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCC > NC_002947/4664735‑4665026 | cacACGCTATGACCATGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACGATGATCTATTTTCTGGTGACCTCTGCGATCGTGCTCTACATCACTCCAGCCGTCGCTGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCgg < 3:179589/147‑1 (MQ=255) ccACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGc > 6:255715/1‑149 (MQ=255) tATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑Tc > 4:112893/1‑149 (MQ=255) cAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAg < 3:112893/149‑1 (MQ=255) gATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGggcac < 7:132806/149‑1 (MQ=255) ttCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑Tc < 8:71992/131‑1 (MQ=255) ttCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑Tc > 7:71992/1‑131 (MQ=255) aGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑a < 5:123490/149‑1 (MQ=255) cTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATg > 6:269779/1‑146 (MQ=255) cTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATg < 5:269779/146‑1 (MQ=255) gACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCCCCCAGCTGCGC‑AGCCTGGCAATGgtggt > 8:8030/1‑147 (MQ=255) ccTGATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCCCACAGTGAGGATTTGACCCATGTACAAGTATATCGGCGACA‑TCGTAAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGtt > 1:249548/1‑148 (MQ=255) gATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCa > 1:64166/1‑149 (MQ=255) tCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCAc < 5:255715/148‑1 (MQ=255) tGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATg < 6:39167/127‑1 (MQ=255) tGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATg > 5:39167/1‑127 (MQ=255) gTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCaa < 1:12423/148‑1 (MQ=255) gACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAgcg > 1:247813/1‑149 (MQ=255) cGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGa > 3:247033/1‑149 (MQ=255) cGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGa > 4:277510/1‑149 (MQ=255) cTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTAAATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTATCCAACGGGTTCAGCCAGCGCGaca > 6:158430/1‑149 (MQ=255) cTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTAAATCGGCGAAA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGCGTTCCGCAAgcgctcca > 6:162934/1‑145 (MQ=255) gATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGCCA‑TCGTTTAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGCGTTCCGCACGCGCGACCCCCt > 4:171401/1‑149 (MQ=255) gATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATgg < 3:221104/115‑1 (MQ=255) gATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATgg > 4:221104/1‑115 (MQ=255) aTCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTAAATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTg < 5:158430/149‑1 (MQ=255) aTCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTAAATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTg < 5:162934/149‑1 (MQ=255) aacaCGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCgaa < 6:163748/149‑1 (MQ=255) cGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAAc < 3:277510/149‑1 (MQ=255) cAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACAGACGTTAAGGGCACCGGCCCCCAGCTCCGC‑AGCATGGCAATGGTGTTGTCCCACGGGTTCCGCAAGCCCGACACCATGAAATACCCCGAACAAccc > 1:241882/1‑149 (MQ=255) aGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCgt < 2:249548/149‑1 (MQ=255) aGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCgt < 2:64166/149‑1 (MQ=255) cGTCGAGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACACAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACACGTGTa > 7:287220/1‑149 (MQ=255) tGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCgcgc < 4:247033/149‑1 (MQ=255) gATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTAcc > 7:260778/1‑149 (MQ=255) tttGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACcgc > 6:148229/1‑149 (MQ=255) gACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATAccc > 4:1709/1‑112 (MQ=255) gACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATAccc < 3:1709/112‑1 (MQ=255) cccATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCg > 6:200729/1‑149 (MQ=255) aaGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGCGAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTcc > 1:64980/1‑149 (MQ=255) gTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTgc < 6:122503/119‑1 (MQ=255) gTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTgc > 5:122503/1‑119 (MQ=255) tatCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCAccc > 7:2498/1‑149 (MQ=255) | CCCACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACACGCCAGCCGTCGCGGCC‑‑CAGTGAGGATTTGACCCATGTTCAAGTATATCGGCGACA‑TCGTTAAGGGCACCGGCACCCAGCTGCGC‑AGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCC > NC_002947/4664735‑4665026 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |