Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,666,493 T→C 8.7% S87P (TCG→CCG)  nuoL → NADH‑quinone oxidoreductase subunit L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,666,4930TC 8.7% 126.5 / 2.4 46S87P (TCG→CCG) nuoLNADH‑quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand):  ref base T (22/20);  new base C (2/2);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

GCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGC  >  NC_002947/4666350‑4666639
                                                                                                                                                |                                                                                                                                                  
gCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGCGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGt                                                                                                                                                >  1:231925/1‑148 (MQ=255)
   tGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCAc                                                                                                                                             >  3:277513/1‑148 (MQ=255)
        gccgccACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCt                                                                                                                                       <  2:69815/149‑1 (MQ=255)
          cgccAGGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGCCGGTCACCACGcc                                                                                                                                       >  7:286520/1‑146 (MQ=255)
               cGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGCGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCgtg                                                                                                                                <  2:231925/149‑1 (MQ=255)
                   cgccTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGa                                                                                                                            <  4:128470/149‑1 (MQ=255)
                    gccTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGCCGGTCACCACGCCGAGCATGGTGAc                                                                                                                           <  8:286520/149‑1 (MQ=255)
                        aCGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGgt                                                                                                                       >  3:225327/1‑149 (MQ=255)
                          gTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCACGCCGAGTATGGTGACCgacag                                                                                                                     >  3:33211/1‑145 (MQ=255)
                          gTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCACGCCGAGTATGGTGACCgacag                                                                                                                     <  4:33211/149‑5 (MQ=255)
                                 ggCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTc                                                                                                              >  5:142983/1‑149 (MQ=255)
                                      ttCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCTGTGTc                                                                                                                    <  7:200586/138‑1 (MQ=255)
                                      ttCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTc                                                                                                                    >  8:200586/1‑138 (MQ=255)
                                         aaCGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCAt                                                                                                                                          <  4:40969/113‑1 (MQ=255)
                                         aaCGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCAt                                                                                                                                          >  3:40969/1‑113 (MQ=255)
                                               gCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGtt                                                                                                <  6:142983/149‑1 (MQ=255)
                                                ccccGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGtt                                                                                                >  1:73929/1‑149 (MQ=255)
                                                ccccGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGCCGGTCACCACGCCGAGCATGGTGACCgaca                                                                                                                      >  8:291595/1‑123 (MQ=38)
                                                ccccGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGCCGGTCACCACGCCGAGCATGGTGACCgaca                                                                                                                      <  7:291595/126‑4 (MQ=38)
                                                 cccGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTc                                                                                               >  1:118452/1‑148 (MQ=255)
                                                 cccGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTc                                                                                               <  2:118452/148‑1 (MQ=255)
                                                              ggcgCGTACAGCCAGCTGCTGCGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCgt                                                                                                                                 <  3:259374/101‑1 (MQ=255)
                                                              ggcgCGTACAGCCAGCTGCTGCGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCgt                                                                                                                                 >  4:259374/1‑101 (MQ=255)
                                                                 gcgTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCg                                                                              >  5:68043/1‑149 (MQ=255)
                                                                 gcgTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCg                                                                              >  3:69199/1‑149 (MQ=255)
                                                                   gTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTg                                                                            <  3:2775/149‑1 (MQ=255)
                                                                         ccagcTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAg                                                                      >  5:313025/1‑149 (MQ=255)
                                                                              tgctgTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCgg                                                                  >  4:55031/1‑148 (MQ=255)
                                                                               gctgTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGc                                                                 >  3:156426/1‑148 (MQ=255)
                                                                                         tGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGctt                                                      <  6:313025/149‑1 (MQ=255)
                                                                                         tGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGctt                                                      >  2:135784/1‑149 (MQ=255)
                                                                                                      gACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTtctc                                                >  5:135604/1‑143 (MQ=255)
                                                                                                      gACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTtctc                                                <  6:135604/143‑1 (MQ=255)
                                                                                                      gACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTaca                                           <  7:168774/148‑1 (MQ=255)
                                                                                                         ggCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCaa                                       >  5:67028/1‑149 (MQ=255)
                                                                                                            ttCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCt                                    >  4:21553/1‑149 (MQ=255)
                                                                                                               gcgcCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGtt                                 <  1:135784/149‑1 (MQ=255)
                                                                                                                 gcCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGcttctt                                                   >  7:263853/1‑129 (MQ=255)
                                                                                                                 gcCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGcttctt                                                   <  8:263853/129‑1 (MQ=255)
                                                                                                                          cACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGc                       <  3:21553/148‑1 (MQ=255)
                                                                                                                           aCCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCAt                     <  2:73929/149‑1 (MQ=255)
                                                                                                                             ccTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGc                   >  8:198321/1‑149 (MQ=255)
                                                                                                                                   ccTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTcc             >  7:66752/1‑149 (MQ=255)
                                                                                                                                            ccTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTg    <  6:67028/149‑1 (MQ=255)
                                                                                                                                            ccTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTg    <  4:277513/149‑1 (MQ=255)
                                                                                                                                            ccTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTg    <  3:55031/149‑1 (MQ=255)
                                                                                                                                                tCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGc  <  4:156426/147‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGC  >  NC_002947/4666350‑4666639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑