Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,666,508 G→A 15.0% G92S (GGC→AGC)  nuoL → NADH‑quinone oxidoreductase subunit L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,666,5080GA15.0% 125.8 / 11.3 53G92S (GGC→AGC) nuoLNADH‑quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand):  ref base G (23/22);  new base A (4/4);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGATAAC  >  NC_002947/4666365‑4666645
                                                                                                                                                |                                                                                                                                         
cGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGCGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCgtg                                                                                                                                      <  2:231925/149‑1 (MQ=255)
    cgccTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGa                                                                                                                                  <  4:128470/149‑1 (MQ=255)
     gccTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGCCGGTCACCACGCCGAGCATGGTGAc                                                                                                                                 <  8:286520/149‑1 (MQ=255)
         aCGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGgt                                                                                                                             >  3:225327/1‑149 (MQ=255)
           gTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCACGCCGAGTATGGTGACCgacag                                                                                                                           <  4:33211/149‑5 (MQ=255)
           gTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCACGCCGAGTATGGTGACCgacag                                                                                                                           >  3:33211/1‑145 (MQ=255)
                  ggCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTc                                                                                                                    >  5:142983/1‑149 (MQ=255)
                       ttCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCTGTGTc                                                                                                                          <  7:200586/138‑1 (MQ=255)
                       ttCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTc                                                                                                                          >  8:200586/1‑138 (MQ=255)
                                gCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGtt                                                                                                      <  6:142983/149‑1 (MQ=255)
                                 ccccGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGtt                                                                                                      >  1:73929/1‑149 (MQ=255)
                                 ccccGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGCCGGTCACCACGCCGAGCATGGTGACCgaca                                                                                                                            >  8:291595/1‑123 (MQ=38)
                                 ccccGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGCCGGTCACCACGCCGAGCATGGTGACCgaca                                                                                                                            <  7:291595/126‑4 (MQ=38)
                                  cccGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTc                                                                                                     >  1:118452/1‑148 (MQ=255)
                                  cccGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTc                                                                                                     <  2:118452/148‑1 (MQ=255)
                                               ggcgCGTACAGCCAGCTGCTGCGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCgt                                                                                                                                       <  3:259374/101‑1 (MQ=255)
                                               ggcgCGTACAGCCAGCTGCTGCGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCgt                                                                                                                                       >  4:259374/1‑101 (MQ=255)
                                                  gcgTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCg                                                                                    >  3:69199/1‑149 (MQ=255)
                                                  gcgTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCg                                                                                    >  5:68043/1‑149 (MQ=255)
                                                    gTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTg                                                                                  <  3:2775/149‑1 (MQ=255)
                                                          ccagcTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAg                                                                            >  5:313025/1‑149 (MQ=255)
                                                               tgctgTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCgg                                                                        >  4:55031/1‑148 (MQ=255)
                                                                gctgTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGc                                                                       >  3:156426/1‑148 (MQ=255)
                                                                          tGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGctt                                                            <  6:313025/149‑1 (MQ=255)
                                                                          tGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGctt                                                            >  2:135784/1‑149 (MQ=255)
                                                                                       gACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTtctc                                                      <  6:135604/143‑1 (MQ=255)
                                                                                       gACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTtctc                                                      >  5:135604/1‑143 (MQ=255)
                                                                                       gACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTaca                                                 <  7:168774/148‑1 (MQ=255)
                                                                                          ggCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCaa                                             >  5:67028/1‑149 (MQ=255)
                                                                                             ttCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCt                                          >  4:21553/1‑149 (MQ=255)
                                                                                                gcgcCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGtt                                       <  1:135784/149‑1 (MQ=255)
                                                                                                  gcCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGcttctt                                                         <  8:263853/129‑1 (MQ=255)
                                                                                                  gcCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGcttctt                                                         >  7:263853/1‑129 (MQ=255)
                                                                                                           cACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGc                             <  3:21553/148‑1 (MQ=255)
                                                                                                            aCCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCAt                           <  2:73929/149‑1 (MQ=255)
                                                                                                              ccTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGc                         >  8:198321/1‑149 (MQ=255)
                                                                                                                    ccTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTcc                   >  7:66752/1‑149 (MQ=255)
                                                                                                                             ccTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTg          <  4:277513/149‑1 (MQ=255)
                                                                                                                             ccTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTg          <  3:55031/149‑1 (MQ=255)
                                                                                                                             ccTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTg          <  6:67028/149‑1 (MQ=255)
                                                                                                                                 tCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGc        <  4:156426/147‑1 (MQ=255)
                                                                                                                                  cGGTCACCACGCCGAGCATGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTgg         >  8:295000/1‑145 (MQ=255)
                                                                                                                                  cGGTCACCACGCCGAGCATGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTgg         <  7:295000/145‑1 (MQ=255)
                                                                                                                                   ggTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGAt     <  6:68043/148‑1 (MQ=255)
                                                                                                                                   ggTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGAt     >  2:138489/1‑148 (MQ=255)
                                                                                                                                   ggTCACCACGCCGAGCATGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGAt     >  5:229917/1‑148 (MQ=255)
                                                                                                                                     tccccATGGGCGGCGTGGTGCCCGGTGTCGGCTTCCTGATGCACCTGTTCGGATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGATAAc  >  4:48267/4‑149 (MQ=255)
                                                                                                                                         cATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTg                                         <  2:10261/106‑1 (MQ=255)
                                                                                                                                         cATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTg                                         >  1:10261/1‑106 (MQ=255)
                                                                                                                                         cATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCAt                                    >  7:13762/1‑111 (MQ=255)
                                                                                                                                         cATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCAt                                    <  8:13762/111‑1 (MQ=255)
                                                                                                                                              tCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATcc             <  6:272161/129‑1 (MQ=255)
                                                                                                                                              tCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATcc             >  5:272161/1‑129 (MQ=255)
                                                                                                                                                |                                                                                                                                         
CGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTC‑GGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGATAAC  >  NC_002947/4666365‑4666645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑