Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,667,164 C→T 7.5% L310L (CTC→CTT)  nuoL → NADH‑quinone oxidoreductase subunit L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,667,1640CT 7.5% 154.4 / 2.1 53L310L (CTC→CTT) nuoLNADH‑quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand):  ref base C (24/25);  new base T (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.92e-01

TGTACCTGATCGCCCGTACCAACGGCCTGTTCCTGCTGGCGCCGGACATC‑‑CTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCTTGCCTCCGGTGCGGTGATCGTTGCCTGCCAC  >  NC_002947/4667028‑4667311
                                                                                                                                          |                                                                                                                                                   
tgtACCTGATCGCCCGTACCAACGGCCTGTTCCTGCTGGCGCCGGACATC‑‑CTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGAcc                                                                                                                                         <  2:179115/149‑1 (MQ=255)
  tACCTGTTCGCCCGTACCAACGGCCTGTTCCTGCTGGGGCCGTTCCCCCGCTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGAcc                                                                                                                                         <  1:105296/149‑1 (MQ=255)
  tACCTGATCGCCCGTACCAACGGCCTGTTCCTGCTGGCGCCGGACATC‑‑CTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCa                                                                                                                                        <  1:250501/148‑1 (MQ=255)
  tACCTGATCGCCCGTACCAACGGCCTGTTCCTGCTGGCGCCGGACATC‑‑CTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCAt                                                                                                                                       >  4:280597/1‑149 (MQ=255)
             ccGTACCAACGGCCTGTTCCTGCTGGCGCCGGACATC‑‑CTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGc                                                                                                                                   >  4:302837/1‑142 (MQ=255)
                    aaCGGCCTGTTCCTGCTGGCGCCGGACATC‑‑CTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACAt                                                                                                                     >  3:257211/1‑149 (MQ=255)
                             ttCCTGCTGGCGCCGGACATC‑‑CTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGc                                                                                                            >  8:14981/1‑149 (MQ=255)
                                   ctgGCGCCGGACATC‑‑CTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCACGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTggg                                                                                                      >  6:192319/1‑149 (MQ=255)
                                   ctgGCGCCGGACATC‑‑CTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTgg                                                                                                       <  7:14981/148‑1 (MQ=255)
                                   ctgGCGCCGGACATC‑‑CTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTgg                                                                                                       <  2:291291/148‑1 (MQ=255)
                                    tgGCGCCGGACATC‑‑CTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGc                                                                                                     >  1:294359/1‑149 (MQ=255)
                                        gcCGGACATC‑‑CTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTgg                                                                                                 <  1:156452/149‑1 (MQ=255)
                                        gcCGGACATC‑‑CTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTgg                                                                                                 <  3:302837/149‑1 (MQ=255)
                                            gACATC‑‑CTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGc                                                                                             >  3:243847/1‑149 (MQ=255)
                                                    cTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTTGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGa                                                                                       >  2:222759/1‑149 (MQ=255)
                                                    cTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTTGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGa                                                                                       >  2:218342/1‑149 (MQ=255)
                                                    cTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTg                                                                                                  >  6:321920/1‑138 (MQ=255)
                                                    cTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTg                                                                                                  <  5:321920/138‑1 (MQ=255)
                                                    cTGCACCTTGTAGGTGTGGTCGGTGGATTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATAAAGCGTATCCTCGACTACTCGACCAAGAGCCAGATAGGCTTCATGTTCCTGGCCATGGGCGTGCTTGCCTGCGa                                                                                       >  5:261366/1‑149 (MQ=255)
                                                         ccTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTg                                                                                                              <  7:112200/121‑1 (MQ=255)
                                                         ccTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTg                                                                                                              >  8:112200/1‑121 (MQ=255)
                                                          cTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACgcggc                                                                                 >  3:181276/1‑149 (MQ=255)
                                                                  tgtgGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTgg                                                                                                 <  2:279562/125‑1 (MQ=255)
                                                                  tgtgGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTgg                                                                                                 >  1:279562/1‑125 (MQ=255)
                                                                        cGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCtga                                                                   >  7:246326/1‑149 (MQ=255)
                                                                          gTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCtgatg                                                                 <  3:280597/149‑1 (MQ=255)
                                                                          gTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCtgat                                                                  >  1:43215/1‑148 (MQ=255)
                                                                               ttGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCa                                                            >  7:52654/1‑149 (MQ=255)
                                                                               ttGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCa                                                            >  3:153823/1‑149 (MQ=255)
                                                                                     cTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTgg                                                                                                 >  2:255152/1‑106 (MQ=255)
                                                                                     cTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGAACATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCAGGGACGCGGCGATCTTCCACCTGATGACCCACGcc                                                        >  4:54856/1‑147 (MQ=255)
                                                                                        gTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCACGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCcttctt                                                   <  5:192319/149‑1 (MQ=255)
                                                                                           cTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCTATCTTCCACCTGATGACCCACGCCTTCTTCaa                                                <  3:145468/149‑1 (MQ=255)
                                                                                              gCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATGCTCGCGTACTCGACCATGAGCCAGATCGGCTACATGTTCGTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGc                                             <  5:154191/149‑1 (MQ=255)
                                                                                                  gCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCctg                                         >  3:101917/1‑149 (MQ=255)
                                                                                                           cgctgGTACAGACCGATATCAAGCGTATCCTTGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCtt                                <  1:222759/149‑1 (MQ=255)
                                                                                                           cgctgGTACAGACCGATATCAAGCGTATCCTTGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCtt                                <  1:218342/149‑1 (MQ=255)
                                                                                                           cgctgGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCtt                                <  4:153823/149‑1 (MQ=255)
                                                                                                           cgctgGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCtt                                <  4:101917/149‑1 (MQ=255)
                                                                                                             ctgGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCTTGc                              <  4:181276/149‑1 (MQ=255)
                                                                                                              tgGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCTTGcc                             <  5:53663/149‑1 (MQ=255)
                                                                                                                  aCAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCAc                                                                       <  4:222389/103‑1 (MQ=255)
                                                                                                                  aCAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCAc                                                                       >  3:222389/1‑103 (MQ=255)
                                                                                                                  aCAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCTTGCCTCcg                         >  3:167798/1‑149 (MQ=255)
                                                                                                                    aGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCTTGCCTCcggt                       <  4:243847/149‑1 (MQ=255)
                                                                                                                      aCCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGt                                                                                               <  7:29804/75‑1 (MQ=255)
                                                                                                                      aCCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGt                                                                                               >  8:29804/1‑75 (MQ=255)
                                                                                                                       ccGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCTTGCCTCcggtgcg                    <  4:167798/149‑1 (MQ=255)
                                                                                                                             tCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCTTGCCTCCGGTGCGGTGATc              >  5:158588/1‑149 (MQ=255)
                                                                                                                             tCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCTTGCCTCCGGTGCGGTGATc              <  6:158588/149‑1 (MQ=255)
                                                                                                                                aGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCTTGCCTCCGGTGCGGTGATCGtt           <  4:257211/149‑1 (MQ=255)
                                                                                                                                        cTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCTTGCCTCCGGTGCGGTGATCGTtgcctgcc    <  2:294359/148‑1 (MQ=255)
                                                                                                                                         tCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGCTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTCCTGTTCCTTGACTCCGGTGCGGTGATCGTTGCCTGccac  >  8:184525/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
TGTACCTGATCGCCCGTACCAACGGCCTGTTCCTGCTGGCGCCGGACATC‑‑CTGCACCTTGTAGGTGTGGTCGGTGGCTTGACCCTGGTACTGGCAGGCTTCGCTGCGCTGGTACAGACCGATATCAAGCGTATCCTCGCCTACTCGACCATGAGCCAGATCGGCTACATGTTCCTGGCCCTGGGCGTGGGTGCCTGGGACGCGGCGATCTTCCACCTGATGACCCACGCCTTCTTCAAGGCCCTGCTGTTCCTTGCCTCCGGTGCGGTGATCGTTGCCTGCCAC  >  NC_002947/4667028‑4667311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑