Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,687,693 C→G 6.7% I223M (ATC→ATG)  PP_4148 → ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,687,6930CG 6.7% 191.0 / 3.8 60I223M (ATC→ATG) PP_4148ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (31/25);  new base G (2/2);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

TGTTCGCCATCCTGCTGATCGTGCTGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACGGCCACGTGTTC  >  NC_002947/4687548‑4687834
                                                                                                                                                 |                                                                                                                                               
tGTTCGCCATCCTGCTGATCGTGCTGTTCGCCGGTGGCCGCTACTTCGACTGGTTCCAGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCAcc                                                                                                                                              >  5:157898/1‑149 (MQ=255)
              ctgATCGTGCTGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCgg                                                                                                                               <  2:179498/149‑1 (MQ=255)
              ctgATCGTGCTGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCg                                                                                                                                <  5:15911/148‑1 (MQ=255)
                gATCGTGCTGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGc                                                                                                                              >  1:76963/1‑148 (MQ=255)
                       cTGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATGACCAGCGC‑GGTGATCGGCag                                                                                                                            >  1:269310/1‑142 (MQ=39)
                       cTGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATGACCAGCGC‑GGTGATCGGCag                                                                                                                            <  2:269310/143‑2 (MQ=39)
                        tGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGc                                                                                                                      >  7:222854/1‑148 (MQ=255)
                               cGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGa                                                                                                              >  8:178423/1‑149 (MQ=255)
                                 gTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCGCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGAcc                                                                                                            <  8:218974/149‑1 (MQ=255)
                                      aGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCctgct                                                                                                       >  5:171631/1‑149 (MQ=255)
                                             tCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATGACCAGCGC‑GGTGATCGGCag                                                                                                                            <  7:54069/121‑2 (MQ=38)
                                             tCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATGACCAGCGC‑GGTGATCGGCag                                                                                                                            >  8:54069/1‑120 (MQ=38)
                                             tCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGAcc                                                                                                   >  4:19350/1‑146 (MQ=255)
                                               gACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGaa                                                                                              >  8:72852/1‑149 (MQ=255)
                                                 cTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACa                                                                                            <  1:80795/149‑1 (MQ=255)
                                                    gTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGcca                                                                                                                    <  1:88656/122‑1 (MQ=255)
                                                    gTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGcca                                                                                                                    >  2:88656/1‑122 (MQ=255)
                                                    gTTACCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCT‑CTGACCAAGAACAGCtt                                                                                        >  7:284187/1‑149 (MQ=255)
                                                     ttCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAAc                                                                                             >  7:55087/1‑144 (MQ=255)
                                                     ttCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAAc                                                                                             <  8:55087/144‑1 (MQ=255)
                                                     ttCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCtt                                                                                        >  5:12264/1‑149 (MQ=255)
                                                         cGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTc                                                                                    <  8:222854/149‑1 (MQ=255)
                                                                     tCACCTCGAACAACTTCGACGAACTGAGCACCACCGTCAAGGTGCTGTACTACTTCTGGCACCTGGTGCTGGCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCT‑CTGACCAAGAACAGCTTCCTCCACGAAATcaaca                                                                       <  8:284187/149‑1 (MQ=255)
                                                                     tCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACtt                                                                                                                         <  3:167552/100‑1 (MQ=255)
                                                                     tCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACtt                                                                                                                         >  4:167552/1‑100 (MQ=255)
                                                                       aCCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATcaac                                                                        <  7:178423/147‑1 (MQ=255)
                                                                         cTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCaacaaac                                                                    <  3:19350/149‑1 (MQ=255)
                                                                            caacaaCTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTTGTCATCGGCAACTTCGCCACCATGACCCTGATGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGt                                                                 <  6:157898/149‑1 (MQ=255)
                                                                            caacaaCTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTc                                                                                    <  5:184498/130‑1 (MQ=255)
                                                                            caacaaCTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTc                                                                                    >  6:184498/1‑130 (MQ=255)
                                                                            caacaaCTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCaacaaaca                                                                   <  6:12264/147‑1 (MQ=255)
                                                                             aacaacTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGa                                                                                                     >  5:177011/1‑112 (MQ=255)
                                                                             aacaacTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGa                                                                                                     <  6:177011/112‑1 (MQ=255)
                                                                                     cgacgaACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑Ca                                                       >  1:221498/1‑149 (MQ=255)
                                                                                             tGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCgccaag                                               >  1:273450/1‑149 (MQ=255)
                                                                                                 caccacCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTcgacg                                                                                >  7:71597/1‑113 (MQ=255)
                                                                                                 caccacCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTcgacg                                                                                <  8:71597/113‑1 (MQ=255)
                                                                                                    caccGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCt                                                                                         <  5:246361/101‑1 (MQ=255)
                                                                                                    caccGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCt                                                                                         >  6:246361/1‑101 (MQ=255)
                                                                                                    caccGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCaacaaa                                                                     <  4:194338/121‑1 (MQ=255)
                                                                                                    caccGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGccaaggccaagg                                        >  4:160974/1‑149 (MQ=255)
                                                                                                      ccGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGc                                      >  8:90091/1‑149 (MQ=255)
                                                                                                         gCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCt                                    >  1:14871/1‑148 (MQ=255)
                                                                                                           aaGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTAc                                                               >  2:93046/1‑120 (MQ=255)
                                                                                                                 cTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGAt                            <  2:76963/148‑1 (MQ=255)
                                                                                                                  tGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCAGACCAAGAACAGCTTCGAAGACGAAATAAACAAAAAGTAAGTGGTACACC‑CCAAAGCCAAGGGCCTGAGCCGAta                           >  1:257326/1‑147 (MQ=37)
                                                                                                                    gACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCAc                         <  6:171631/148‑1 (MQ=255)
                                                                                                                    gACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCAc                         <  2:57668/148‑1 (MQ=255)
                                                                                                                       tactTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCgcc                                                  <  6:178431/120‑1 (MQ=255)
                                                                                                                       tactTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCgcc                                                  >  5:178431/1‑120 (MQ=255)
                                                                                                                         ctTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTg                    <  7:72852/148‑1 (MQ=255)
                                                                                                                         ctTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTg                    <  3:160974/148‑1 (MQ=255)
                                                                                                                          ttCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCt                  >  8:762/1‑149 (MQ=255)
                                                                                                                               gCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACg             >  6:271400/1‑149 (MQ=255)
                                                                                                                                 aCCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACGGc           >  1:122471/1‑149 (MQ=255)
                                                                                                                                   cTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACGGCCa         >  1:281446/1‑149 (MQ=255)
                                                                                                                                   cTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACGGCCa         >  8:84379/1‑149 (MQ=255)
                                                                                                                                          tgcCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACGGCCACGTGTTc  >  2:100614/1‑149 (MQ=255)
                                                                                                                                              gATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGt                                                                 <  3:121004/83‑1 (MQ=255)
                                                                                                                                              gATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGt                                                                 >  4:121004/1‑83 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TGTTCGCCATCCTGCTGATCGTGCTGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACGGCCACGTGTTC  >  NC_002947/4687548‑4687834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑