| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,687,705 | C→G | 6.4% | V227V (GTC→GTG) | PP_4148 → | ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,687,705 | 0 | C | G | 6.4% | 202.9 / 3.3 | 62 | V227V (GTC→GTG) | PP_4148 | ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base C (31/27); new base G (2/2); total (33/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CTGATCGTGCTGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACGGCCACGTGTTCCGCAACGCCATGCTGCTG > NC_002947/4687562‑4687852 | ctgATCGTGCTGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCgg < 2:179498/149‑1 (MQ=255)ctgATCGTGCTGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCg < 5:15911/148‑1 (MQ=255) gATCGTGCTGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGc > 1:76963/1‑148 (MQ=255) cTGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATGACCAGCGC‑GGTGATCGGCag > 1:269310/1‑142 (MQ=39) cTGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATGACCAGCGC‑GGTGATCGGCag < 2:269310/143‑2 (MQ=39) tGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGc > 7:222854/1‑148 (MQ=255) cGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGa > 8:178423/1‑149 (MQ=255) gTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCGCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGAcc < 8:218974/149‑1 (MQ=255) aGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCctgct > 5:171631/1‑149 (MQ=255) tCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATGACCAGCGC‑GGTGATCGGCag < 7:54069/121‑2 (MQ=38) tCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATGACCAGCGC‑GGTGATCGGCag > 8:54069/1‑120 (MQ=38) tCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGAcc > 4:19350/1‑146 (MQ=255) gACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGaa > 8:72852/1‑149 (MQ=255) cTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACa < 1:80795/149‑1 (MQ=255) gTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGcca < 1:88656/122‑1 (MQ=255) gTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGcca > 2:88656/1‑122 (MQ=255) gTTACCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCT‑CTGACCAAGAACAGCtt > 7:284187/1‑149 (MQ=255) ttCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAAc > 7:55087/1‑144 (MQ=255) ttCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAAc < 8:55087/144‑1 (MQ=255) ttCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCtt > 5:12264/1‑149 (MQ=255) cGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTc < 8:222854/149‑1 (MQ=255) tCACCTCGAACAACTTCGACGAACTGAGCACCACCGTCAAGGTGCTGTACTACTTCTGGCACCTGGTGCTGGCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCT‑CTGACCAAGAACAGCTTCCTCCACGAAATcaaca < 8:284187/149‑1 (MQ=255) tCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACtt < 3:167552/100‑1 (MQ=255) tCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACtt > 4:167552/1‑100 (MQ=255) aCCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATcaac < 7:178423/147‑1 (MQ=255) cTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCaacaaac < 3:19350/149‑1 (MQ=255) caacaaCTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTTGTCATCGGCAACTTCGCCACCATGACCCTGATGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGt < 6:157898/149‑1 (MQ=255) caacaaCTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTc > 6:184498/1‑130 (MQ=255) caacaaCTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTc < 5:184498/130‑1 (MQ=255) caacaaCTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCaacaaaca < 6:12264/147‑1 (MQ=255) aacaacTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGa > 5:177011/1‑112 (MQ=255) aacaacTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGa < 6:177011/112‑1 (MQ=255) cgacgaACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑Ca > 1:221498/1‑149 (MQ=255) tGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCgccaag > 1:273450/1‑149 (MQ=255) caccacCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTcgacg < 8:71597/113‑1 (MQ=255) caccacCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTcgacg > 7:71597/1‑113 (MQ=255) caccGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCt < 5:246361/101‑1 (MQ=255) caccGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCt > 6:246361/1‑101 (MQ=255) caccGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCaacaaa < 4:194338/121‑1 (MQ=255) caccGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGccaaggccaagg > 4:160974/1‑149 (MQ=255) ccGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGc > 8:90091/1‑149 (MQ=255) gCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCt > 1:14871/1‑148 (MQ=255) aaGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTAc > 2:93046/1‑120 (MQ=255) cTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGAt < 2:76963/148‑1 (MQ=255) tGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCAGACCAAGAACAGCTTCGAAGACGAAATAAACAAAAAGTAAGTGGTACACC‑CCAAAGCCAAGGGCCTGAGCCGAta > 1:257326/1‑147 (MQ=37) gACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCAc < 6:171631/148‑1 (MQ=255) gACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCAc < 2:57668/148‑1 (MQ=255) tactTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCgcc < 6:178431/120‑1 (MQ=255) tactTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCgcc > 5:178431/1‑120 (MQ=255) ctTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTg < 3:160974/148‑1 (MQ=255) ctTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTg < 7:72852/148‑1 (MQ=255) ttCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCt > 8:762/1‑149 (MQ=255) gCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACg > 6:271400/1‑149 (MQ=255) aCCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACGGc > 1:122471/1‑149 (MQ=255) cTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACGGCCa > 1:281446/1‑149 (MQ=255) cTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACGGCCa > 8:84379/1‑149 (MQ=255) tgcCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACGGCCACGTGTTc > 2:100614/1‑149 (MQ=255) gATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGt < 3:121004/83‑1 (MQ=255) gATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGt > 4:121004/1‑83 (MQ=255) aCC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACGGCCACGTGTTCCGCAACGc < 2:273450/149‑1 (MQ=255) gTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACGGCCACGTGTTCCGCAACGCCATgctgct > 4:303338/1‑149 (MQ=255) tCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACGGCCACGTGTTCCGCAACGCCATGctgctg < 7:762/149‑1 (MQ=255) | CTGATCGTGCTGTTCGCCGGTGGCAGCTACTTCGACTGGTTCCCGCTGCGTGGCCTCACCTCCAACAACTTCGACGAACTGAGCACCACCGGCAAGGTGCTGGACTACTTCTGGCACCTGGTGCTGCCGATCACC‑GCGCTGGTCATCGGCAACTTCGCCACCATGACCCTGCTGACCAAGAACAGCTTCCTCGACGAAATCAACAAACAGTACGTGGT‑CACCGCCAAGGCCAAGGGCCTGAGCCGATCACGGGTGCTGTACGGCCACGTGTTCCGCAACGCCATGCTGCTG > NC_002947/4687562‑4687852 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |