Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,726,572 T→C 8.3% V270V (GTA→GTG)  brnQ ← branched‑chain amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,726,5720TC 8.3% 135.8 / 2.7 48V270V (GTA→GTG) brnQbranched‑chain amino acid transporter
Reads supporting (aligned to +/- strand):  ref base T (20/24);  new base C (2/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

GATCACCACCAGCGCGCGGTACGACAGCGGCAGGATCTGGCTGAAGTACTCGGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGCC  >  NC_002947/4726425‑4726720
                                                                                                                                                   |                                                                                                                                                    
gATCACCACCAGCGCGCGGTACGACAGCGGCAGGATCTGGCTGAAGTACTCGGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTa                                                                                                                                                     >  5:88729/1‑149 (MQ=255)
     ccaccaGCGCGCGGTACGACAGCGGCAGGATCTGGCTGAAGTACTCGGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAg                                                                                                                                                >  6:79684/1‑149 (MQ=255)
        ccaGCGCGCGGTACGACAGCGGCAGGATCTGGCTGAAGTACTCGGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGCACGTAGGCa                                                                                                                                             >  5:253403/1‑149 (MQ=255)
                 ggTACGACAGCGGCAGGATCTGGCTGAAGTACTCGGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGt                                                                                                                                                  <  2:93653/135‑1 (MQ=255)
                 ggTACGACAGCGGCAGGATCTGGCTGAAGTACTCGGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGt                                                                                                                                                  >  1:93653/1‑135 (MQ=255)
                               aGGATCTGGCTGAAGTACTCGGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGCACGTAGGCATGCAGCACCGCCGCACCGTtggt                                                                                                                      <  6:253403/149‑1 (MQ=255)
                                 gATCTGGCTGAAGTACTCGGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGGGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTtggtgg                                                                                                                    <  6:234160/149‑1 (MQ=255)
                                       gCTGAAGTACTCGGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTc                                                                                                                <  5:242305/147‑1 (MQ=255)
                                           aaGTACTCGGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCcgc                                                                                                          <  7:205856/149‑1 (MQ=255)
                                            aGTACTCGGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCcgcg                                                                                                         <  6:88729/149‑1 (MQ=255)
                                             gTACTCGGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGa                                                                                                        >  6:16896/1‑149 (MQ=255)
                                               aCTCGGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATg                                                                                                      >  7:147100/1‑149 (MQ=255)
                                                 tCGGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCGAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCACGacatc                                                                                                    >  1:200646/1‑145 (MQ=255)
                                                   ggCGCAGGCACAGGTCAGGCCAACCGCGGTCACCATGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTc                                                                                                    <  7:131790/147‑1 (MQ=255)
                                                   ggCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTtggt                                                                                                                      >  8:189991/1‑129 (MQ=255)
                                                   ggCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTtggt                                                                                                                      <  7:189991/129‑1 (MQ=255)
                                                   ggCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGt                                                                                                  <  4:197491/149‑1 (MQ=255)
                                                          gCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCTAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCACGACATCGCGGCtgac                                                                                           <  2:200646/149‑3 (MQ=255)
                                                           cacaGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCtggtg                                                                                          <  6:4253/149‑1 (MQ=255)
                                                           cacaGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCtggtg                                                                                          <  5:79684/149‑1 (MQ=255)
                                                           cacaGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCtggtg                                                                                          <  5:16896/149‑1 (MQ=255)
                                                                gTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCAcc                                                                                     >  5:304340/1‑149 (MQ=255)
                                                                          ccGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGGACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAAc                                                                           <  8:147100/149‑1 (MQ=255)
                                                                                  aCCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGAt                                                                   >  2:136581/1‑149 (MQ=255)
                                                                                    caggcaggCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATg                                                                  >  1:114714/1‑148 (MQ=255)
                                                                                       gcaggcCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGa                                                              >  8:113927/1‑149 (MQ=255)
                                                                                         aggcCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATgcagca                                                                                                                                      <  5:79091/75‑1 (MQ=255)
                                                                                         aggcCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATgcagca                                                                                                                                      >  6:79091/1‑75 (MQ=255)
                                                                                              aGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGCACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGc                                                       >  1:290811/1‑149 (MQ=255)
                                                                                               gcgcGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGc                                                       >  4:135498/1‑148 (MQ=255)
                                                                                                    aTCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGccc                                                 <  6:304340/149‑1 (MQ=255)
                                                                                                     tCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGccc                                                 >  1:89279/1‑148 (MQ=255)
                                                                                                        gCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGaca                                             <  7:113927/149‑1 (MQ=255)
                                                                                                                aGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGAt                                     >  5:133018/1‑149 (MQ=255)
                                                                                                                  gAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGAt                                     >  2:280288/1‑147 (MQ=255)
                                                                                                                     gCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGc                                <  1:280288/149‑1 (MQ=255)
                                                                                                                         cTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGc                            <  2:114714/149‑1 (MQ=255)
                                                                                                                             ccAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATg                                                                  <  2:164818/107‑1 (MQ=255)
                                                                                                                             ccAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCgat                         >  4:309043/1‑148 (MQ=255)
                                                                                                                                  gAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGc                   <  3:135498/149‑1 (MQ=255)
                                                                                                                                   aGCCGAAGGTATGCTGCACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCg                  <  2:290811/149‑1 (MQ=255)
                                                                                                                                       gAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGc              >  7:114079/1‑149 (MQ=255)
                                                                                                                                         aGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCgg            <  1:136581/149‑1 (MQ=255)
                                                                                                                                              tGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAAt       <  3:309043/149‑1 (MQ=255)
                                                                                                                                                 tGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGac                                              >  7:274068/1‑107 (MQ=255)
                                                                                                                                                 tGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGac                                              >  7:269747/1‑107 (MQ=255)
                                                                                                                                                 tGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGac                                              <  8:269747/107‑1 (MQ=255)
                                                                                                                                                 tGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGac                                              <  8:274068/107‑1 (MQ=255)
                                                                                                                                                   tACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCCGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGcc  >  3:189917/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GATCACCACCAGCGCGCGGTACGACAGCGGCAGGATCTGGCTGAAGTACTCGGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGTATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGCC  >  NC_002947/4726425‑4726720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑