Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,740,841 T→A 18.3% intergenic (+78/+675) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8410TA18.3% 65.3 / 10.2 38intergenic (+78/+675)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (14/16);  new base A (3/4);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.30e-01

CCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGT  >  NC_002947/4740701‑4740982
                                                                                                                                            |                                                                                                                                              
gctcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcgcg                                                                                                                                        >  1:262817/4‑148 (MQ=255)
 ctcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                                                      <  2:262817/147‑1 (MQ=255)
 cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                                                      <  5:268110/149‑1 (MQ=255)
  gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAag                                                                                                                                     <  1:116456/149‑1 (MQ=255)
   cgcCAGGTGTACACCGCCGAGCAGAAGCGCGACATCGTGGCCCTTAAACACCCCAATTAAGCGGCAGACCCCGAACGAAAGAAGGCTGCCCTCGGGCTG‑CTTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCATGGCCGCTAaga                                                                                                                                    <  4:209163/149‑1 (MQ=255)
   cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                                                                    <  2:76326/149‑1 (MQ=38)
    gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                                                                   <  1:67472/149‑1 (MQ=38)
           gTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTTAGCGGCCTTGGCCGCGAGGcggccggcc                                                                                                                            >  6:23162/1‑148 (MQ=255)
                                       gTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTtctc                                                                                               >  6:234655/1‑149 (MQ=255)
                                          gCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTccgc                                                                                            >  2:34467/1‑149 (MQ=255)
                                            ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTccgccc                                                                                          >  1:88941/1‑149 (MQ=255)
                                              tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGttttt                                                                                                  >  8:10300/1‑139 (MQ=39)
                                              tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGttttt                                                                                                  <  7:10300/139‑1 (MQ=39)
                                              tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCATGCACGCGAAGAGGCCGGCCCAG‑CCACCACAAAACCTCAGTTTTTCTccgccccc                                                                                        >  3:72864/1‑147 (MQ=255)
                                               gAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCt                                                                                       >  1:9706/1‑149 (MQ=255)
                                                     ccGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTTCCCGCGAAGAGGCCGGCACAC‑ACACCACAAAACCTCACTTTTTCTACGCCCGCTCACGCa                                                                                 >  2:4719/1‑149 (MQ=255)
                                                              aGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCACTTTTTCTCCGCCCGCTCACGCAGCCCTTTc                                                                         <  1:26177/148‑1 (MQ=255)
                                                               gCTGCAGTAGCCGAACGCAAGATGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGGGCCGGCCTCTGCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                                                       <  3:287179/149‑1 (MQ=255)
                                                                cTGCATTTGGCGAACCCAAGACGGCGGCCCTCGGGCATCC‑TTTTTTATTTTGCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAATAGGCCGGCACAGTCCACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                                                       <  1:233215/149‑1 (MQ=255)
                                                                cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                                                      <  2:9706/149‑1 (MQ=255)
                                                                             aCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTg                                                                                                                                              <  2:38903/65‑1 (MQ=25)
                                                                                aaaaaagGCTGCCCGAGGGCAGCC‑TTCTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                                                       >  3:225010/5‑132 (MQ=38)
                                                                                aaaaaagGCTGCCCGAGGGCAGCC‑TTCTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                                                       <  4:225010/128‑1 (MQ=38)
                                                                                      ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACATAGCGGCCGTGGCCGCGAAGAGGGCGGCACAG‑ACACCACAAAACCACCATTTTTATACGCCCCCAAACCCACACCGTTAAGGGTTTTTAACGGCGCATacacca                                                >  4:12613/1‑149 (MQ=255)
                                                                                         tGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACg                                              >  2:208593/1‑148 (MQ=255)
                                                                                                gggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt                                      <  1:4719/149‑1 (MQ=255)
                                                                                                     agccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGcc                                  <  1:34467/145‑1 (MQ=255)
                                                                                                      gccttatttATTTGTCGTGGGCCGGCCTCTTCGCGGCCTTGCCCGCTATGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGcca                                 <  1:161412/143‑1 (MQ=255)
                                                                                                       ccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                                                   >  5:222992/3‑98 (MQ=38)
                                                                                                       ccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                                                   <  6:222992/96‑1 (MQ=38)
                                                                                                                         tGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTacc              <  4:72864/149‑1 (MQ=255)
                                                                                                                              gCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTg         >  2:141385/1‑149 (MQ=255)
                                                                                                                                ctctTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGc       <  5:234655/149‑1 (MQ=255)
                                                                                                                                 tctTCGCGGGCAAGGCCGCGAAGAGGCCGGCACAG‑GCACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCt      >  6:255515/1‑149 (MQ=255)
                                                                                                                                 tctTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCt      >  2:247999/1‑149 (MQ=255)
                                                                                                                                   ttCGCGGGCAAGGCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCg    >  5:136523/1‑149 (MQ=255)
                                                                                                                                   ttCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGAGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCAACTGGCTCg    >  3:234850/1‑149 (MQ=255)
                                                                                                                                     cgcgGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGgt  <  1:141385/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                              
CCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAG‑ACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGT  >  NC_002947/4740701‑4740982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑