Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 432,995 A→T 11.5% intergenic (+20/+27) PP_0355 → / ← glcB two‑component system response regulator/malate synthase G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947432,9950AT11.5% 138.7 / 7.8 52intergenic (+20/+27)PP_0355/glcBtwo‑component system response regulator/malate synthase G
Reads supporting (aligned to +/- strand):  ref base A (26/20);  new base T (3/3);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

GGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCCACGTTG  >  NC_002947/432852‑433142
                                                                                                                                               |                                                                                                                                                   
gggCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGCCTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGt                                                                                                                                                >  4:97039/1‑149 (MQ=255)
       acCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGAGGATATGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTg                                                                                                                                         >  1:93833/1‑149 (MQ=255)
        ccGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTgg                                                                                                                                        >  1:112225/1‑149 (MQ=255)
         cGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTTTTACGGTCCCCCTGgc                                                                                                                                       <  1:271110/149‑1 (MQ=255)
          gATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGgca                                                                                                                                      <  8:81146/149‑1 (MQ=255)
            tgtgGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGt                                                                                                                                                <  7:23979/137‑1 (MQ=255)
            tgtgGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGt                                                                                                                                                >  8:23979/1‑137 (MQ=255)
                gAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGAt                                                                                                                                >  4:14355/1‑149 (MQ=255)
                         gTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTAc                                                                                                                       <  3:14355/149‑1 (MQ=255)
                          tGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGTATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTAc                                                                                                                       <  4:222011/148‑1 (MQ=255)
                          tGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGAACTGGGCAAGCTGTTGGAGCTCGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACg                                                                                                                                                  >  3:20574/1‑121 (MQ=255)
                               gggATGCACCTGGGGGGGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAAccc                                                                                                                  <  3:167444/146‑1 (MQ=255)
                                 gATGCACCGGGGGGTGGGGGACTTCCTCCCCAACCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGCATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGtt                                                                                                               <  2:93833/149‑1 (MQ=255)
                                    gCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTAc                                                                                                             <  2:112225/148‑1 (MQ=255)
                                     cACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTAcgc                                                                                                           <  5:236464/149‑1 (MQ=255)
                                          gggggTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTTTTACGGTc                                                                                                                                               >  1:20537/1‑108 (MQ=255)
                                          gggggTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTTTTACGGTc                                                                                                                                               >  1:15590/1‑108 (MQ=255)
                                          gggggTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTTTTACGGTc                                                                                                                                               <  2:15590/108‑1 (MQ=255)
                                          gggggTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTTTTACGGTc                                                                                                                                               <  2:20537/108‑1 (MQ=255)
                                           ggggTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATCATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCtt                                                                                                      <  7:10711/148‑1 (MQ=255)
                                                ggtggACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTc                                                                                                >  1:306574/1‑149 (MQ=255)
                                                  tggACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTcgc                                                                                              >  4:77672/1‑149 (MQ=255)
                                                   ggACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAAc                                                                                                                    <  6:160141/126‑1 (MQ=255)
                                                   ggACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAAc                                                                                                                    >  5:160141/1‑126 (MQ=255)
                                                    gACTTCGTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGTGGTGGTTAACAAATAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTcgcg                                                                                             <  2:306574/148‑1 (MQ=255)
                                                       ttCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGAtgctgc                                                                                                                           >  8:206622/1‑115 (MQ=255)
                                                       ttCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGAtgctgc                                                                                                                           <  7:206622/115‑1 (MQ=255)
                                                           tGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGAcggc                                                                                     >  8:10614/1‑149 (MQ=255)
                                                               cAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTg                                                                                 >  1:18191/1‑149 (MQ=255)
                                                                      gTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTaac                                                                          >  1:271073/1‑147 (MQ=255)
                                                                       tGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCg                                                                         <  2:271073/149‑1 (MQ=255)
                                                                                  aaGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATaa                                                              >  2:61032/1‑149 (MQ=255)
                                                                                                  tGAACAAAGAACTGAAAATTGGATGAGGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGttgcttgc                                              <  2:223766/149‑1 (MQ=255)
                                                                                                     aCAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGttgctt                                                >  7:289581/1‑144 (MQ=255)
                                                                                                     aCAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGttgctt                                                <  8:289581/144‑1 (MQ=255)
                                                                                                     aCAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCCTAAAACGGTCCCCCTGGCAGCAGATGCTGCATACAAACCGTTACGCGCCTTGAACTAGCCTCGACGCCGGACAAGTACCGGCTCGGTATAACCGTTCGGTTTCTTGCCAc                                           >  8:83338/1‑149 (MQ=255)
                                                                                                      cAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCAcc                                          >  1:308760/1‑149 (MQ=255)
                                                                                                       aaaGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCt                                         >  7:161695/1‑149 (MQ=255)
                                                                                                       aaaGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCt                                         >  1:149333/1‑149 (MQ=255)
                                                                                                       aaaGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCt                                         <  3:97039/149‑1 (MQ=255)
                                                                                                       aaaGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCt                                         >  1:57204/1‑149 (MQ=255)
                                                                                                            aCTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCAc                                    <  3:77672/149‑1 (MQ=255)
                                                                                                             cTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACt                                   <  7:10614/149‑1 (MQ=255)
                                                                                                               gAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTCCCACCTTCCACTAc                                 >  8:261868/1‑149 (MQ=255)
                                                                                                                aaaaTTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTAc                                 >  5:186661/1‑148 (MQ=255)
                                                                                                                         tgatgCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCcaca                       <  2:308760/149‑1 (MQ=255)
                                                                                                                         tgatgCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCca                         >  5:34615/1‑147 (MQ=255)
                                                                                                                           atgCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAg                      <  7:83338/148‑1 (MQ=255)
                                                                                                                               gcCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAgccgcc                 >  7:80179/1‑149 (MQ=255)
                                                                                                                                    ggggACCGTTTTACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGTCACCTTCCACTACCAGTTCCACAGCCGCCTGGaa            >  6:207734/1‑149 (MQ=255)
                                                                                                                                          cGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCCAc      >  8:98019/1‑149 (MQ=255)
                                                                                                                                            tAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCCACGt    >  1:68315/1‑149 (MQ=255)
                                                                                                                                             aaaaCGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCCACGtt   >  8:204174/1‑149 (MQ=255)
                                                                                                                                              aaaCGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCCACGtt   <  2:267364/148‑1 (MQ=255)
                                                                                                                                               aaCGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGCCGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTACACAGCCGACGGGAACGCCACGtgg  >  1:246850/1‑146 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCCACGTTG  >  NC_002947/432852‑433142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑