| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,796,627 | C→T | 17.9% | intergenic (‑185/+33) | pvdH ← / ← PP_4224 | diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,796,627 | 0 | C | T | 17.9% | 80.7 / 11.8 | 39 | intergenic (‑185/+33) | pvdH/PP_4224 | diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base C (14/18); new base T (3/4); total (17/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.26e-01 | |||||||||||
GGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCC > NC_002947/4796479‑4796772 | ggACAGGGGTAAATCCTGGCTTGCAAGCGGATGGGCGGGTATCGCTTTGGAAACGCTTGAGGGGGGGGAGGATTTAGCGGTTGTTACGCGA‑TTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCa < 8:133229/149‑1 (MQ=255) gggTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgc > 3:139703/1‑149 (MQ=255) ggTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCcgc < 3:128657/148‑1 (MQ=37) gTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGACCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgcg > 3:95806/1‑148 (MQ=255) tAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGaa < 2:207221/149‑1 (MQ=255) aaTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAaga < 7:172716/149‑1 (MQ=255) aaTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAaga < 3:293129/149‑1 (MQ=255) gcgAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCTCCTACGGGCGGGTTTACCcgc < 3:189853/137‑1 (MQ=38) gcgAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCTCCTACGGGCGGGTTTACCcgc > 4:189853/1‑137 (MQ=38) cgAGCAAGCGGATGGGCGTTTATCGCTTTGGAAACGGTTGTGTGGGGGGAGGATTTAGCGGTTGTTACGCTATTTTATTTTTGTCTGTGCTGACCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCTAAGAGGCCAGca < 4:95806/149‑1 (MQ=255) aaGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgc > 4:295036/1‑131 (MQ=255) aaGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgc < 3:295036/131‑1 (MQ=255) aaGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGGTCACCCGGGAAGAGGGCCGCACAGGc > 6:10964/1‑149 (MQ=255) aaGCGGATGGGCGGGTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGc < 5:10964/149‑1 (MQ=255) ggCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCTCCTACGGGCGGGTTTACCCGCGaa > 6:225474/1‑125 (MQ=37) ggCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACa < 1:246868/148‑1 (MQ=255) ggCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGAGTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCTCCTACGGGCGGGTTTACCCGCGaa < 5:225474/125‑1 (MQ=25) ggTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAaga < 6:135298/124‑1 (MQ=255) ggTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAaga > 5:135298/1‑124 (MQ=255) aTCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGAAGGAGCCGGTTCACCCGCGAAGAGGCCAGCAAAGGCGATAAACATCAATTcc > 6:291314/1‑149 (MQ=255) cTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAg > 2:195641/1‑148 (MQ=255) gAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGaa < 2:212347/109‑1 (MQ=21) aaCGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATc > 8:38810/1‑132 (MQ=255) aaCGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATc < 7:38810/132‑1 (MQ=255) ggggAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGc > 2:272963/1‑99 (MQ=255) ggggAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGc < 1:272963/99‑1 (MQ=255) gggAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCTGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGc > 7:128501/1‑108 (MQ=255) gggAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCTGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGc < 8:128501/108‑1 (MQ=255) gaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGccc < 7:216540/120‑1 (MQ=255) gaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGccc > 8:216540/1‑120 (MQ=255) tttAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGc < 1:195641/149‑1 (MQ=255) aGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCaa < 4:139703/148‑1 (MQ=255) tttttGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCg < 5:93071/105‑1 (MQ=255) tttttGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCg > 6:93071/1‑105 (MQ=255) ttGTCTGTGCTGGCCTCTTGCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCCGCACAGGCGATAAACATCAATGCCCAGGCCGCGCCGGTACCGGCAACAAAACCCGCAGGCCGGCCCTCGGGCGGGCAAAgcg > 4:38879/1‑149 (MQ=37) tcgccTGTGCTGGCCTCTT‑CGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAgcgc > 3:187939/5‑149 (MQ=21) gTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc > 4:162229/1‑148 (MQ=255) gcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTg < 3:38879/149‑1 (MQ=255) gTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGcc < 3:162229/149‑1 (MQ=255) | GGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTT‑CGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCC > NC_002947/4796479‑4796772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |