| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,858,519 | A→C | 8.6% | intergenic (‑25/+200) | PP_mr50 ← / ← ligA | Bacteria_large_SRP ncRNA/DNA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,858,519 | 0 | A | C | 8.6% | 168.4 / 3.9 | 56 | intergenic (‑25/+200) | PP_mr50/ligA | Bacteria_large_SRP ncRNA/DNA ligase |
| Reads supporting (aligned to +/- strand): ref base A (26/25); new base C (3/2); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTTTCCGACCCCAGAAATGGAGGCGGCCCCACCAGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAG > NC_002947/4858371‑4858667 | gTTTTCCGACCCCAGAAATGGAGGCGGCCCCACCAGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGa < 1:55218/149‑1 (MQ=255) cccAGAAATGGAGGCGGCCCCACCAGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTg > 2:176409/1‑149 (MQ=255) cccAGAAATGGAGGCGGCCCCACCAGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTg > 2:166201/1‑149 (MQ=255) cccAGAAATGGAGGCGGCCCCACCAGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGACTg > 8:299416/1‑149 (MQ=255) ccAGAAATGGAGGCGGCCCCACCAGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGCTCAGCGAgaca < 2:18418/149‑4 (MQ=255) ccAGAAATGGAGGCGGCCCCACCAGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTgg > 8:169547/1‑149 (MQ=255) ccAGAAATGGAGGCGGCCCCACCAGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCAATACATGTCTCGCTGAGCAGCGAGTgg < 1:169658/149‑1 (MQ=255) cggcggcCCCACCAGCCACACCCCGGCACTCGATGTTCCCGCTAAGGCTGCTCCCTTCCGGGCCTGACCATGTTAACGGGTAATCAATGCCGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGt < 1:87432/148‑1 (MQ=255) ggcggcCCCACCAGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTAc < 8:108085/149‑1 (MQ=255) cccACCAGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCt > 1:11303/1‑149 (MQ=255) accaGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGTGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTg < 1:39937/149‑1 (MQ=255) accaGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGTGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCATCGAGTGGCGCCATTGTACCGGTCTTTg < 7:299416/149‑1 (MQ=255) accaGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTg < 8:182967/149‑1 (MQ=255) accaGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTg < 2:11303/149‑1 (MQ=255) aGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAg < 4:125433/149‑1 (MQ=255) gCCACACCCCGTCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGc < 6:306365/149‑1 (MQ=255) acacCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAg > 2:142265/1‑145 (MQ=255) acCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATa > 1:142919/1‑149 (MQ=255) cGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAAc < 2:142919/149‑1 (MQ=255) gATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCGGACCAGGTGAACGGGTAATCCATG‑CGGCGGGACCGATGGGGCCACCACTACATGTCTCGCTGCGCAGCGCGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCgg > 5:232738/1‑149 (MQ=255) ccGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGaaa > 7:294232/1‑148 (MQ=255) cGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGaaa > 7:76138/1‑147 (MQ=255) cTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGCTCAGCGAga > 2:97120/1‑95 (MQ=255) cTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGCTCAGCGAga < 1:97120/96‑2 (MQ=255) tGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGtcat < 1:142265/149‑1 (MQ=255) gctCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCAttt > 4:283810/1‑149 (MQ=255) ctCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTtc > 1:45507/1‑149 (MQ=255) ccTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAAc < 4:111786/119‑1 (MQ=255) ccTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAAc > 3:111786/1‑119 (MQ=255) ccGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAAt < 3:283810/147‑1 (MQ=255) ccGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAAt < 8:76138/147‑1 (MQ=255) ccGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAAt < 1:166201/147‑1 (MQ=255) ggCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACtt < 7:169547/149‑1 (MQ=255) cTGACCAGGTTAACGGGTAATCAATG‑C‑GGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGt > 7:87781/1‑147 (MQ=255) ggTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTc > 1:155702/1‑148 (MQ=255) cgatAACCGGAAATCAATG‑CCGTGCCACCGCAGGCGCCACCACAACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTCCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCg < 8:280221/146‑1 (MQ=255) gTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCt > 8:80990/1‑149 (MQ=255) tAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTg > 7:1779/1‑149 (MQ=255) tCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCa > 1:165334/1‑130 (MQ=255) tCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCa < 2:165334/130‑1 (MQ=255) tCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGAATTTGGAGCAGATACAACCACTGCGGAAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGAc > 7:298304/1‑149 (MQ=255) tG‑CGGGGGGACCTATGGGGGCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGc < 7:285275/149‑1 (MQ=255) ggggggACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCctc < 8:294232/149‑1 (MQ=255) gggACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTc < 1:176409/149‑1 (MQ=255) aCCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTcgcg > 8:186830/1‑149 (MQ=255) aCCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTcgcg > 4:301413/1‑149 (MQ=255) aTGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGt > 5:134142/1‑148 (MQ=255) caccacTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGc < 7:186830/149‑1 (MQ=255) accacTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTc < 5:266342/110‑1 (MQ=255) accacTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCt > 4:275173/1‑149 (MQ=255) accacTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCt < 3:301413/149‑1 (MQ=255) cATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGt > 7:240960/1‑96 (MQ=255) cATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGt < 8:240960/96‑1 (MQ=255) aTGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACggg > 4:119856/1‑149 (MQ=255) cactcGCTGCTCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGc > 3:261545/3‑148 (MQ=255) tctcGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGa > 6:164520/1‑149 (MQ=255) tcGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTAGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGAcc > 1:55279/1‑149 (MQ=255) agcagcGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAg < 8:1779/149‑1 (MQ=255) agcagcGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAg < 8:87781/149‑1 (MQ=255) | GTTTTCCGACCCCAGAAATGGAGGCGGCCCCACCAGCCACACCCCGGCACTCGATGTTCCCGCTATGGCTGCTCCCTTCCGGGCCTGACCAGGTTAACGGGTAATCAATG‑CGGGGGGACCGATGGGGCCACCACTACATGTCTCGCTGAGCAGCGAGTGGCGCCATTGTACCGGTCTTTGGAGCAGATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAG > NC_002947/4858371‑4858667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |