Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,900,069	G→A	7.9%	intergenic (+30/‑468)	PP_4308 → / → PP_4309	AsnC family transcriptional regulator/NCS1 family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,900,069	0	G	A	7.9%	178.7 / 4.5	63	intergenic (+30/‑468)	PP_4308/PP_4309	AsnC family transcriptional regulator/NCS1 family transporter
Reads supporting (aligned to +/- strand):  ref base G (29/29);  new base A (3/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.81e-01

TGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGACCAAATGATCAAC  >  NC_002947/4899924‑4900216                                                                                                                                                  |                                                                                                                                                     tgCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGc                                                                                                                                                   >  5:325757/1‑149 (MQ=255)        gATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGACTCCTGTGCTGGCCTCTTCGCAGGCGTGCCcgcg                                                                                                                                              >  4:65448/1‑147 (MQ=255)          tGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAaga                                                                                                                                          <  5:298150/149‑1 (MQ=255)           ggAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAagag                                                                                                                                         <  6:309252/149‑1 (MQ=255)             agcagcTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGc                                                                                                                                       >  6:197249/1‑149 (MQ=255)                          ccggcgcGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGaa                                                                                                                                            <  2:82383/125‑1 (MQ=255)                          ccTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGaa                                                                                                                            >  1:189560/1‑147 (MQ=255)                          ccTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGaa                                                                                                                            >  6:165054/1‑147 (MQ=255)                                 gACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGACTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGa                                                                                                                     <  3:65448/147‑1 (MQ=255)                                  aCCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTAGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTa                                                                                                                 >  5:75598/1‑149 (MQ=255)                                    cTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACGCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCAAGTAAAGGAAAAGATGA‑GTagt                                                                                                               >  7:264302/1‑147 (MQ=255)                                     tCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGaa                                                                                                                                            <  4:94360/120‑1 (MQ=255)                                     tCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGaa                                                                                                                                            >  3:94360/1‑120 (MQ=255)                                                 tGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTa                                                                                                        >  7:218368/1‑143 (MQ=255)                                                 tGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTa                                                                                                        <  8:218368/143‑1 (MQ=255)                                                   gCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAgg                                                                                                                                        >  1:46663/1‑110 (MQ=255)                                                   gCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAgg                                                                                                                                        <  2:46663/110‑1 (MQ=255)                                                     cGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCa                                                                                                                                     <  7:244640/111‑1 (MQ=255)                                                     cGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCa                                                                                                                                     >  8:244640/1‑111 (MQ=255)                                                      gagaCCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACg                                                                                              >  2:170767/1‑148 (MQ=255)                                                       agaCCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGt                                                                                             >  4:107380/1‑148 (MQ=255)                                                                  gcgATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTAAACAAAAACGTAACCATAgtggc                                                                                 >  8:288990/1‑145 (MQ=255)                                                                    gATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAaga                                                                                                                                          >  6:314115/1‑91 (MQ=255)                                                                    gATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAaga                                                                                                                                          <  5:314115/91‑1 (MQ=255)                                                                        atcaAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCAtttt                                                                           >  2:83602/1‑149 (MQ=255)                                                                           aagaagACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTtgt                                                                        <  6:325757/149‑1 (MQ=255)                                                                           aagaagACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTtgt                                                                        <  2:189560/149‑1 (MQ=255)                                                                           aagaagACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTtgt                                                                        <  5:197249/149‑1 (MQ=255)                                                                           aagaagACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCAGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTtgt                                                                        <  6:75598/149‑1 (MQ=255)                                                                             gaagaCCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTtgtgt                                                                      >  3:141087/1‑149 (MQ=255)                                                                                gaCCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAACAGGCCAGTACAGGAAAAACTGAGGTATTTTTTTTACACAAAAACGTAACCATAGGTGCCATTTTtgtgtg                                                                     >  1:102252/1‑148 (MQ=39)                                                                                   cccGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTCA‑GAATTTTTTTTAAACAAAAAAGTAACCCTAGGTTCCATTTTTTTTTTCACtt                                                                >  1:260775/1‑149 (MQ=38)                                                                                     cGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTttc                                                              <  7:103103/149‑1 (MQ=255)                                                                                          aaGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAg                                                         <  1:170767/149‑1 (MQ=255)                                                                                          aaGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAg                                                         >  2:96361/1‑149 (MQ=255)                                                                                             cggcggTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTgg                                                      <  1:83602/149‑1 (MQ=255)                                                                                               gcggTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGgcg                                                    <  4:141087/149‑1 (MQ=255)                                                                                                       cgccAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAAtt                                            <  5:122042/149‑1 (MQ=255)                                                                                                       caccAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAAtt                                            >  8:20510/3‑149 (MQ=255)                                                                                                           aaTGGATTGATTGGCTCCTGTGCTTGCCTCTTCGCAGGCGTGCCCGCGAAGATGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCttt                                        <  7:7860/149‑1 (MQ=255)                                                                                                            aTGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCTCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTa                                       <  6:201802/149‑1 (MQ=255)                                                                                                                attgattgGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCAtttt                                                                           <  1:183140/109‑1 (MQ=255)                                                                                                                attgattgGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCAtttt                                                                           >  2:183140/1‑109 (MQ=255)                                                                                                                     ttgGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTc                              >  4:246396/1‑149 (MQ=255)                                                                                                                       ggCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTcc                             <  5:165054/148‑1 (MQ=255)                                                                                                                        gCTCCTGTGCTGGCCTGTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACATGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCa                            <  7:288990/148‑1 (MQ=255)                                                                                                                              gtgCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCTTAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTa                     >  4:300602/1‑149 (MQ=255)                                                                                                                                gCTGGCCTCTTCGCAGGCGTGCCCTCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGtt                                        <  1:232221/128‑1 (MQ=255)                                                                                                                                gCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGtt                                        >  2:232221/1‑128 (MQ=255)                                                                                                                                    gCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTAtttt                 <  7:20510/147‑1 (MQ=255)                                                                                                                                     ccTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCTTAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGAc              <  3:300602/149‑1 (MQ=255)                                                                                                                                      ctctTCGCGGGCACGCCTGCGAAGAGGCCAGCACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTa                                       >  5:247527/1‑123 (MQ=38)                                                                                                                                      ctctTCGCGGGCACGCCTGCGAAGAGGCCAGCACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTa                                       <  6:247527/123‑1 (MQ=38)                                                                                                                                      ctctTCGCAGGCGTGTCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGt                                         >  3:293884/1‑121 (MQ=255)                                                                                                                                      ctctTCGCAGGCGTGTCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGt                                         <  4:293884/121‑1 (MQ=255)                                                                                                                                      ctctTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGAc              >  7:225893/1‑148 (MQ=255)                                                                                                                                       tctTCGCGGGCACGCCTGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTATTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGAcc             >  5:210018/1‑148 (MQ=255)                                                                                                                                       tctTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGAcc             >  7:233890/1‑148 (MQ=255)                                                                                                                                         ttCGCGGGCACGCCTGCGAAGAGGCCAGCACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCaa                                              >  6:22055/1‑113 (MQ=38)                                                                                                                                         ttCGCGGGCACGCCTGCGAAGAGGCCAGCACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCaa                                              <  5:22055/113‑1 (MQ=38)                                                                                                                                            gCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGACCAAATGa       <  3:246396/149‑1 (MQ=255)                                                                                                                                                gCGTGCCCGCGAAGAGGCCAGTGCAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCAATTTTTGTGTGCACTTTCTTCAGTGGCGccc                                                 >  7:199526/1‑103 (MQ=255)                                                                                                                                                 cGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGACCAAATGATCAAc  <  2:102252/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                     TGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGA‑GTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGACCAAATGATCAAC  >  NC_002947/4899924‑4900216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑