| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,909,922 | C→G | 9.8% | intergenic (+315/+164) | PP_4318 → / ← PP_4319 | transposase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,909,922 | 0 | C | G | 9.8% | 128.6 / 6.6 | 51 | intergenic (+315/+164) | PP_4318/PP_4319 | transposase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (18/28); new base G (3/2); total (21/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
GCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAAAGCAGAAGCAGAAGCAGAAGCAGCAGAAGC > NC_002947/4909780‑4910069 | gCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATgggg < 4:168610/149‑1 (MQ=32) cccTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGc < 7:95519/149‑1 (MQ=32) cggcggGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCCGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAgc < 1:20750/149‑1 (MQ=17) gcggGTCACTTTTTGTCAAACGCGACAGAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAgcgc < 7:12536/149‑1 (MQ=17) cACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACg > 5:273935/1‑149 (MQ=32) aCTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACTGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACg < 2:80848/148‑1 (MQ=17) tCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCAt > 2:18658/1‑149 (MQ=32) aaaCGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGc > 7:244726/1‑149 (MQ=32) aaaCGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGc > 6:106993/1‑149 (MQ=32) aCGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGccc > 6:230671/1‑149 (MQ=32) aCGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGATACGGTCTTGCTACCGCGAGTACGCGCTGCAAGCCCCAACCATGGGGCCTCAGCGCTTGACCGGCATCCATGccc > 6:69997/1‑149 (MQ=11) aaaaaGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTACCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCt > 1:91671/1‑149 (MQ=17) aaaGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTcc < 8:244726/149‑1 (MQ=32) aGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCt > 4:238974/1‑149 (MQ=32) tAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTc > 8:98018/1‑149 (MQ=33) aCCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCg < 5:228460/148‑1 (MQ=34) cAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATgggg > 1:246170/1‑104 (MQ=32) cAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATgggg < 2:246170/104‑1 (MQ=32) aaaCGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGcc > 3:112484/1‑148 (MQ=34) tcatcCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTc < 8:214472/148‑1 (MQ=35) tcatcCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCt < 1:18658/149‑1 (MQ=37) tcatcCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCAACCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTc < 3:110728/148‑1 (MQ=25) atcCGGCCCCTGCGCTACGCTCCGGGGTTCCCAAGCAACGGTATTTCAACCCGGAGTAACCCCATCCGGCCCCAACCAAGGGGCCGAAGCGCATTACCGGCCTACAAGCCCCTACCCACCCATCGCAAGCCCAGCCCTCCGccataag > 2:224056/1‑143 (MQ=2) cGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAg < 3:238974/148‑1 (MQ=37) cgcTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCt < 7:98018/148‑1 (MQ=37) gcTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGc < 6:273935/149‑1 (MQ=37) tCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTc > 3:252422/1‑104 (MQ=31) tCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTc < 4:252422/104‑1 (MQ=31) ggggTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGaa < 8:8400/149‑1 (MQ=37) gggTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCCCCTCCCTTCGCACGCCCGGCGCTCg > 6:241383/1‑116 (MQ=255) gggTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTGGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCg < 5:241383/116‑1 (MQ=25) ccTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACcg < 3:157095/148‑1 (MQ=39) gCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCgcgcgc < 5:230671/149‑1 (MQ=40) tACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCCTCCATGCCCGTCACCTCCCTTTGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCt > 3:87557/1‑148 (MQ=255) cGGTGTTGCTCCCGGGAGAACGCGCTGCAGGCCGCATCCATGGTGCCTCAGCGCTTGACTGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGAGCTCGGCCTTCTGAAGTCTCAATCTGCGTCGCCTGAACTACCGCGCGCTTAc < 7:32891/149‑1 (MQ=14) cGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTa < 4:112484/148‑1 (MQ=39) gTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTAc > 3:200096/1‑147 (MQ=39) gTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACaa < 6:47334/149‑1 (MQ=40) tCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCCTCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAg < 4:200096/149‑1 (MQ=255) gCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTTTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGc < 1:224056/146‑1 (MQ=255) gAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaag < 5:106993/149‑1 (MQ=255) gctgaGGCCCCATGGATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaa < 6:171837/135‑1 (MQ=255) gctgaGGCCCCATGGATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaa > 5:171837/5‑139 (MQ=255) tgaGGCCCCATGGATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaagcagaagcag > 3:132701/3‑147 (MQ=255) cAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCGTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaagcagaagcagaag < 5:69997/149‑1 (MQ=255) cAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGTAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaagcagaagcagaa < 6:163242/148‑1 (MQ=255) aGGCCCCATGGATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaagcagaagcagaag < 6:153829/148‑1 (MQ=255) aGGCCCCATGGATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaagcagaagcagaag > 5:153829/1‑148 (MQ=255) gCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCg < 5:158811/86‑1 (MQ=38) gCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCg > 6:158811/1‑86 (MQ=38) gCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaagcagaagcagaagcag > 7:20499/1‑149 (MQ=255) ccatccatGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaagcagaagcagaagcag > 3:60306/1‑146 (MQ=255) catccatGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAAAGCAGAAGCAGAagcagaagcagcaga < 8:20499/149‑1 (MQ=255) catGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAAAGCAGAAGCAGAagcagaagcagcagaagc < 2:91671/148‑1 (MQ=255) | GCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAAAGCAGAAGCAGAAGCAGAAGCAGCAGAAGC > NC_002947/4909780‑4910069 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |