Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,917,689	A→G	8.1%	I30V (ATC→GTC) ‡	PP_4328 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,917,689	0	A	G	8.1%	212.9 / 7.0	74	I30V (ATC→GTC) ‡	PP_4328	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (36/32);  new base G (3/3);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GGCATTATTACACGCGCCGCCGCGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAACGCCA  >  NC_002947/4917541‑4917833                                                                                                                                                     |                                                                                                                                                 ggCATTATTACACGCGCCGCCGCGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGa                                                                                                                                                  >  1:299412/1‑149 (MQ=255)    attattACACGCGCCGCCGCGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATcc                                                                                                                                               <  3:227012/149‑1 (MQ=255)        ttaCACGCGCCGCCGCGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGgc                                                                                                                                           <  2:299412/149‑1 (MQ=255)            acGCGCCGCCGCGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGGATCAGGCGCAg                                                                                                                                       <  6:142966/149‑1 (MQ=255)            acGCGCCGCCGCGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAg                                                                                                                                       >  3:22349/1‑149 (MQ=255)              gcgcCGCTGCGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGcc                                                                                                                                     >  6:144562/1‑149 (MQ=255)              gcgcCGCCGCGCTGCCAAGAGGCCTGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGcc                                                                                                                                     >  7:43397/1‑149 (MQ=255)                   gccgcGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGt                                                                                                                                <  7:33867/149‑1 (MQ=255)                    ccgcGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGtt                                                                                                                               <  4:22349/149‑1 (MQ=255)                      gcgcTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGc                                                                                                                             <  2:151980/149‑1 (MQ=255)                          tGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAGGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAg                                                                                                                          >  6:183750/1‑148 (MQ=255)                           gCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGGATCAGGCGCAg                                                                                                                                       <  1:233867/134‑1 (MQ=255)                           gCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGGATCAGGCGCAg                                                                                                                                       >  2:233867/1‑134 (MQ=255)                            ccAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCt                                                                                                                        >  4:191336/1‑148 (MQ=255)                                 agGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGc                                                                                                                  <  1:29930/149‑1 (MQ=255)                                              cAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCTCGCAAg                                                                                                     <  5:326876/149‑1 (MQ=255)                                                       taCAATCATGACTGAAATCAATTCTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCTTGAAGGCGGGCATGTCCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTTGCTCCTGGCGAAACCGCGCAAGCCGAGGt                                                                                              <  4:192346/147‑1 (MQ=255)                                                       taCAATAATGACTGAAATCAATAGTATCGGCGCACAAACAGCTATCAACAGCCAAGCCAAGAAGGCGGGCATGACCGGCGAACTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTACTGGCGAAACCGCGCAAGACGAGGtta                                                                                            >  3:192346/1‑148 (MQ=255)                                                           aTCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATc                                                                                        <  1:54588/149‑1 (MQ=255)                                                             cATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGt                                                                                      >  1:10606/1‑149 (MQ=255)                                                               tGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTg                                                                                    <  3:191336/149‑1 (MQ=255)                                                               tGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTg                                                                                    <  5:144562/149‑1 (MQ=255)                                                               tGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTg                                                                                    <  6:185596/149‑1 (MQ=255)                                                                 aCTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAGGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGc                                                                                   <  5:183750/148‑1 (MQ=255)                                                                  cTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGt                                                                                 >  7:5159/1‑149 (MQ=255)                                                                    gAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCt                                                                                                                        <  6:196964/108‑1 (MQ=255)                                                                    gAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCt                                                                                                                        >  5:196964/1‑108 (MQ=255)                                                                        tCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACg                                                                           >  7:269856/1‑149 (MQ=255)                                                                          aaTAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTgg                                                                                                                   <  4:71242/107‑1 (MQ=255)                                                                          aaTAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGtt                                                                                     >  5:192911/1‑137 (MQ=255)                                                                          aaTAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGtt                                                                                     <  6:192911/137‑1 (MQ=255)                                                                          aaTAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACggg                                                                         >  3:212005/1‑149 (MQ=255)                                                                          aaTAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACggg                                                                         >  1:50464/1‑149 (MQ=255)                                                                               tctcGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGtt                                                                                     <  2:188078/132‑1 (MQ=255)                                                                               tctcGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGtt                                                                                     >  1:188078/1‑132 (MQ=255)                                                                               tctcGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAg                                                                     >  2:302894/1‑148 (MQ=255)                                                                                   ggCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCATGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGa                                                                   <  8:43397/146‑1 (MQ=255)                                                                                     cgcACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCTCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTc                                                              <  8:5159/149‑1 (MQ=255)                                                                                      gcACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAg                                                                                                >  2:186927/1‑114 (MQ=255)                                                                                      gcACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAg                                                                                                <  1:186927/114‑1 (MQ=255)                                                                                          aaaCCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTAt                                                                                         <  2:35031/117‑1 (MQ=255)                                                                                          aaaCCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTAt                                                                                         >  1:35031/1‑117 (MQ=255)                                                                                          aaaCCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTg                                                         >  7:157937/1‑149 (MQ=255)                                                                                           aaCCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTgg                                                        <  2:10606/149‑1 (MQ=255)                                                                                             ccGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTg                                                      >  7:249518/1‑149 (MQ=255)                                                                                              cgcgATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGc                                                     >  8:130785/1‑149 (MQ=255)                                                                                                     aaCAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGc                                               <  8:249518/148‑1 (MQ=255)                                                                                                       cAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGa                                            >  3:104785/1‑149 (MQ=255)                                                                                                          ccaagccaTGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATg                                          >  8:238545/1‑148 (MQ=255)                                                                                                            aagccaTGAAGGCGGGCATGACCGGCGATCTGCTGCGCATGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAg                                       <  3:197060/149‑1 (MQ=255)                                                                                                               ccaTGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGc                                     >  6:32886/1‑148 (MQ=255)                                                                                                                  tGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCGGCTGGTGCTACg                                                 >  4:198648/1‑133 (MQ=255)                                                                                                                  tGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCGGCTGGTGCTACg                                                 <  3:198648/133‑1 (MQ=255)                                                                                                                  tGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCACGccgtt                                 >  7:278525/1‑146 (MQ=255)                                                                                                                      ggCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCg                             <  2:50464/149‑1 (MQ=255)                                                                                                                               gACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGAcc                     >  1:116317/1‑148 (MQ=255)                                                                                                                                aCCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGAcc                     >  3:269098/1‑147 (MQ=255)                                                                                                                                  cGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCGGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGc                 >  7:160075/1‑149 (MQ=255)                                                                                                                                  cGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGc                 <  4:104785/149‑1 (MQ=255)                                                                                                                                    gCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCtt               >  4:106625/1‑149 (MQ=255)                                                                                                                                     cGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTc              <  8:157937/149‑1 (MQ=255)                                                                                                                                        tCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAgg           >  5:290063/1‑149 (MQ=255)                                                                                                                                            ctgcGCCTGGATCAGGCGCAGCCTGGGTTGGTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGCGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCaa       >  1:29525/1‑149 (MQ=255)                                                                                                                                            ctgcGCCTGGATCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGt                                                       <  3:45307/101‑1 (MQ=255)                                                                                                                                            ctgcGCCTGGATCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGt                                                       >  4:45307/1‑101 (MQ=255)                                                                                                                                            ctgcGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAg            <  8:269856/144‑1 (MQ=255)                                                                                                                                             tgcGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAAc      >  4:63708/1‑149 (MQ=255)                                                                                                                                             tgcGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAAc      >  1:135428/1‑149 (MQ=255)                                                                                                                                             tgcGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAAc      <  2:116317/149‑1 (MQ=255)                                                                                                                                              gcgcCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTg                                             <  8:25951/109‑1 (MQ=255)                                                                                                                                              gcgcCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTg                                             >  7:25951/1‑109 (MQ=255)                                                                                                                                               cgcCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCACCGGCGTGCAGACCCAGCTTCAGGCCAACgc    >  6:94867/1‑149 (MQ=255)                                                                                                                                                 ccTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAACgcca  >  2:128203/1‑149 (MQ=255)                                                                                                                                                   tGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAACgcca  <  5:287369/147‑1 (MQ=255)                                                                                                                                                   tGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAACgcca  >  6:287369/1‑147 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GGCATTATTACACGCGCCGCCGCGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAACGCCA  >  NC_002947/4917541‑4917833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑