Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,917,691 C→T 8.1% I30I (ATC→ATT) ‡ PP_4328 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,917,6910CT 8.1% 213.2 / 6.5 74I30I (ATC→ATT) ‡PP_4328hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (37/31);  new base T (3/3);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01

ATTATTACACGCGCCGCCGCGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAACGCCAGCCAGC  >  NC_002947/4917544‑4917839
                                                                                                                                                   |                                                                                                                                                    
attattACACGCGCCGCCGCGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATcc                                                                                                                                                     <  3:227012/149‑1 (MQ=255)
    ttaCACGCGCCGCCGCGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGgc                                                                                                                                                 <  2:299412/149‑1 (MQ=255)
        acGCGCCGCCGCGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGGATCAGGCGCAg                                                                                                                                             <  6:142966/149‑1 (MQ=255)
        acGCGCCGCCGCGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAg                                                                                                                                             >  3:22349/1‑149 (MQ=255)
          gcgcCGCTGCGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGcc                                                                                                                                           >  6:144562/1‑149 (MQ=255)
          gcgcCGCCGCGCTGCCAAGAGGCCTGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGcc                                                                                                                                           >  7:43397/1‑149 (MQ=255)
               gccgcGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGt                                                                                                                                      <  7:33867/149‑1 (MQ=255)
                ccgcGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGtt                                                                                                                                     <  4:22349/149‑1 (MQ=255)
                  gcgcTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGc                                                                                                                                   <  2:151980/149‑1 (MQ=255)
                      tGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAGGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAg                                                                                                                                >  6:183750/1‑148 (MQ=255)
                       gCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGGATCAGGCGCAg                                                                                                                                             <  1:233867/134‑1 (MQ=255)
                       gCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGGATCAGGCGCAg                                                                                                                                             >  2:233867/1‑134 (MQ=255)
                        ccAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCt                                                                                                                              >  4:191336/1‑148 (MQ=255)
                             agGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGc                                                                                                                        <  1:29930/149‑1 (MQ=255)
                                          cAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCTCGCAAg                                                                                                           <  5:326876/149‑1 (MQ=255)
                                                   taCAATCATGACTGAAATCAATTCTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCTTGAAGGCGGGCATGTCCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTTGCTCCTGGCGAAACCGCGCAAGCCGAGGt                                                                                                    <  4:192346/147‑1 (MQ=255)
                                                   taCAATAATGACTGAAATCAATAGTATCGGCGCACAAACAGCTATCAACAGCCAAGCCAAGAAGGCGGGCATGACCGGCGAACTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTACTGGCGAAACCGCGCAAGACGAGGtta                                                                                                  >  3:192346/1‑148 (MQ=255)
                                                       aTCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATc                                                                                              <  1:54588/149‑1 (MQ=255)
                                                         cATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGt                                                                                            >  1:10606/1‑149 (MQ=255)
                                                           tGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTg                                                                                          <  6:185596/149‑1 (MQ=255)
                                                           tGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTg                                                                                          <  5:144562/149‑1 (MQ=255)
                                                           tGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTg                                                                                          <  3:191336/149‑1 (MQ=255)
                                                             aCTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAGGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGc                                                                                         <  5:183750/148‑1 (MQ=255)
                                                              cTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGt                                                                                       >  7:5159/1‑149 (MQ=255)
                                                                gAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCt                                                                                                                              >  5:196964/1‑108 (MQ=255)
                                                                gAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCt                                                                                                                              <  6:196964/108‑1 (MQ=255)
                                                                    tCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACg                                                                                 >  7:269856/1‑149 (MQ=255)
                                                                      aaTAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTgg                                                                                                                         <  4:71242/107‑1 (MQ=255)
                                                                      aaTAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGtt                                                                                           >  5:192911/1‑137 (MQ=255)
                                                                      aaTAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGtt                                                                                           <  6:192911/137‑1 (MQ=255)
                                                                      aaTAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACggg                                                                               >  1:50464/1‑149 (MQ=255)
                                                                      aaTAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACggg                                                                               >  3:212005/1‑149 (MQ=255)
                                                                           tctcGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGtt                                                                                           <  2:188078/132‑1 (MQ=255)
                                                                           tctcGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGtt                                                                                           >  1:188078/1‑132 (MQ=255)
                                                                           tctcGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAg                                                                           >  2:302894/1‑148 (MQ=255)
                                                                               ggCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCATGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGa                                                                         <  8:43397/146‑1 (MQ=255)
                                                                                 cgcACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCTCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTc                                                                    <  8:5159/149‑1 (MQ=255)
                                                                                  gcACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAg                                                                                                      <  1:186927/114‑1 (MQ=255)
                                                                                  gcACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAg                                                                                                      >  2:186927/1‑114 (MQ=255)
                                                                                      aaaCCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTAt                                                                                               >  1:35031/1‑117 (MQ=255)
                                                                                      aaaCCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTAt                                                                                               <  2:35031/117‑1 (MQ=255)
                                                                                      aaaCCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTg                                                               >  7:157937/1‑149 (MQ=255)
                                                                                       aaCCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTgg                                                              <  2:10606/149‑1 (MQ=255)
                                                                                         ccGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTg                                                            >  7:249518/1‑149 (MQ=255)
                                                                                          cgcgATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGc                                                           >  8:130785/1‑149 (MQ=255)
                                                                                                 aaCAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGc                                                     <  8:249518/148‑1 (MQ=255)
                                                                                                   cAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGa                                                  >  3:104785/1‑149 (MQ=255)
                                                                                                      ccaagccaTGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATg                                                >  8:238545/1‑148 (MQ=255)
                                                                                                        aagccaTGAAGGCGGGCATGACCGGCGATCTGCTGCGCATGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAg                                             <  3:197060/149‑1 (MQ=255)
                                                                                                           ccaTGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGc                                           >  6:32886/1‑148 (MQ=255)
                                                                                                              tGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCGGCTGGTGCTACg                                                       >  4:198648/1‑133 (MQ=255)
                                                                                                              tGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCGGCTGGTGCTACg                                                       <  3:198648/133‑1 (MQ=255)
                                                                                                              tGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCACGccgtt                                       >  7:278525/1‑146 (MQ=255)
                                                                                                                  ggCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCg                                   <  2:50464/149‑1 (MQ=255)
                                                                                                                           gACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGAcc                           >  1:116317/1‑148 (MQ=255)
                                                                                                                            aCCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGAcc                           >  3:269098/1‑147 (MQ=255)
                                                                                                                              cGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCGGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGc                       >  7:160075/1‑149 (MQ=255)
                                                                                                                              cGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGc                       <  4:104785/149‑1 (MQ=255)
                                                                                                                                gCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCtt                     >  4:106625/1‑149 (MQ=255)
                                                                                                                                 cGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTc                    <  8:157937/149‑1 (MQ=255)
                                                                                                                                    tCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAgg                 >  5:290063/1‑149 (MQ=255)
                                                                                                                                        ctgcGCCTGGATCAGGCGCAGCCTGGGTTGGTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGCGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCaa             >  1:29525/1‑149 (MQ=255)
                                                                                                                                        ctgcGCCTGGATCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGt                                                             <  3:45307/101‑1 (MQ=255)
                                                                                                                                        ctgcGCCTGGATCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGt                                                             >  4:45307/1‑101 (MQ=255)
                                                                                                                                        ctgcGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAg                  <  8:269856/144‑1 (MQ=255)
                                                                                                                                         tgcGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAAc            >  1:135428/1‑149 (MQ=255)
                                                                                                                                         tgcGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAAc            <  2:116317/149‑1 (MQ=255)
                                                                                                                                         tgcGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAAc            >  4:63708/1‑149 (MQ=255)
                                                                                                                                          gcgcCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTg                                                   >  7:25951/1‑109 (MQ=255)
                                                                                                                                          gcgcCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTg                                                   <  8:25951/109‑1 (MQ=255)
                                                                                                                                           cgcCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCACCGGCGTGCAGACCCAGCTTCAGGCCAACgc          >  6:94867/1‑149 (MQ=255)
                                                                                                                                             ccTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAACgcca        >  2:128203/1‑149 (MQ=255)
                                                                                                                                               tGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAACgcca        >  6:287369/1‑147 (MQ=255)
                                                                                                                                               tGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAACgcca        <  5:287369/147‑1 (MQ=255)
                                                                                                                                                  tCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAACGccagccag   >  2:37878/1‑149 (MQ=255)
                                                                                                                                                   ccAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAACGCcagccagc  >  7:161059/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATTATTACACGCGCCGCCGCGCTGCCAAGAGGCCGGGCTCGACAGGTTGTATACAATCATGACTGAAATCAATAGTCTCGGCGCACAAACCGCGATCAACAGCCAAGCCATGAAGGCGGGCATGACCGGCGATCTGCTGCGCCTGATCCAGGCGCAGCCTGGGTTGCTAGCTCCTGGCGAAACCGCGCAAGCCGAGGTTTTATCGTTGCGTCAGACGGGCCAGGACTTTCAGCTGGTGCTACGGCTGATGCAGGCCAACGGCGTGCAGACCCAGCTTCAGGCCAACGCCAGCCAGC  >  NC_002947/4917544‑4917839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑