Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,971,955	C→G	13.5%	intergenic (‑390/+27)	PP_4379 ← / ← flgL	3‑oxoacyl‑ACP synthase/flagellar hook‑associated protein FlgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,971,955	0	C	G	13.5%	136.4 / 14.3	52	intergenic (‑390/+27)	PP_4379/flgL	3‑oxoacyl‑ACP synthase/flagellar hook‑associated protein FlgL
Reads supporting (aligned to +/- strand):  ref base C (26/19);  new base G (3/4);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

GCACTGGACCCCGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGTCAGTATCGCCAA  >  NC_002947/4971809‑4972100                                                                                                                                                   |                                                                                                                                                  gcACTGGACCCCGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGAAAGGGCTGCgcag                                                                                                                                                 >  1:109156/1‑149 (MQ=255)   aCTGGACCCCGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCgcagca                                                                                                                                               <  2:109156/149‑1 (MQ=255)   aCTGGACCCCGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCAT‑‑CGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCagcagc                                                                                                                                             <  3:287497/149‑1 (MQ=255)        aCCCCGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCTGCGCAGccct                                                                                                                                          <  3:278548/149‑2 (MQ=255)        aCCCCGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGcccc                                                                                                                                          <  4:2332/149‑1 (MQ=255)           ccGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCTGCGCAGccctatc                                                                                                                                       <  5:242291/149‑5 (MQ=255)              aaTGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATc                                                                                                                                    <  1:310064/149‑1 (MQ=255)               aTGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATca                                                                                                                                   <  3:190259/149‑1 (MQ=255)                  aTCAGCTTCGCAGCAGATGTCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATcagg                                                                                                                                 <  6:40507/148‑1 (MQ=255)                  aTCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATcagg                                                                                                                                 >  5:226591/1‑148 (MQ=255)                      gCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATcaggcagg                                                                                                                             >  1:73312/1‑148 (MQ=255)                             agcagATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGAt                                                                                                                        >  2:29372/1‑146 (MQ=255)                              gcagATATCGATGGGGCCGCATCGCGGCCCTTTCGCCACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGATGACTACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGa                                                                                                                    <  5:306014/149‑1 (MQ=255)                                     tCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTc                                                                                                             <  1:143363/149‑1 (MQ=255)                                       gaaagggCCGCGATGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCg                                                                                                            >  8:134063/5‑148 (MQ=255)                                          ggggCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGaa                                                                                                          >  1:41956/1‑147 (MQ=255)                                           gggCCGCATCGCGGCCCTTTCGAGACGCAAGGACGCTCCCAAGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCGTGTGTCGCGATAGGCCTGCGCAGCAGCCCCAGCATCAGGAAGGCAGATTAGATCTTGTCGAACAg                                                                                                       >  5:31648/1‑149 (MQ=255)                                            ggCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATGAGGCAGGCAGATTAGATCTTGTCGAACAgg                                                                                                      <  1:29372/149‑1 (MQ=255)                                                  aTCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAGTACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGc                                                                                                >  4:43038/1‑149 (MQ=255)                                                   tCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCt                                                                                               >  6:237126/1‑149 (MQ=255)                                                   tCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCt                                                                                               <  4:125877/149‑1 (MQ=255)                                                              tCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATgc                                                                                      <  7:134063/147‑1 (MQ=255)                                                                gcgACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACa                                                                                                        >  3:147556/1‑127 (MQ=255)                                                                gcgACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACa                                                                                                        <  4:147556/127‑1 (MQ=255)                                                                  gACGCAAGGCCTCTCCCACCAATACGCCACCTCCAGGAGAGAGGACCACCTGTGGGGGCCTTGTGTCGCGATCGGGCTGCGCAGCAGCCCCAGCATCAGCCAGGCAGAATAGAGCTAGTCCACAACGCATAGCTGAGAAATGCGCGCa                                                                                 >  4:237835/1‑148 (MQ=255)                                                                     gCAAGGCGGCTCCCACGAATACGCCACCTCCATGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAAc                                                                              <  3:237835/148‑1 (MQ=255)                                                                       aaGGCCGCTCCCACGATTACGCCACCTACAGGAGAGAGGACGACCTGTGGTGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGc                                                                            >  7:237640/1‑148 (MQ=255)                                                                       aaGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCCATAGGGCTGCGCAGCAGCCGCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGc                                                                            >  7:242076/1‑148 (MQ=255)                                                                           ccGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGtt                                                                       >  6:245148/1‑149 (MQ=255)                                                                               tCCCACGAATACGCCACCTCCAGGAGCGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAAGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCg                                                                    >  1:168937/1‑148 (MQ=255)                                                                                 ccACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCa                                                                          <  2:79361/140‑1 (MQ=255)                                                                                 ccACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCa                                                                          >  1:79361/1‑140 (MQ=255)                                                                                 ccACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGaa                                                                  <  5:245148/148‑1 (MQ=255)                                                                                       aTACGCCACCTCCAGGAGAGAGGTCGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGATCAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTc                                                             >  7:289960/1‑147 (MQ=255)                                                                                                       agagagGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAg                                           >  1:184214/1‑149 (MQ=255)                                                                                                        gagagGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAgg                                          <  5:237126/149‑1 (MQ=255)                                                                                                          gagGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGt                                        >  8:146626/1‑149 (MQ=255)                                                                                                          gagGACGACCTGGGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGt                                        <  2:41956/149‑1 (MQ=255)                                                                                                                gacCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCt                                   >  4:131822/1‑148 (MQ=255)                                                                                                                  ccTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATTCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGaa                                >  8:170581/1‑149 (MQ=255)                                                                                                                             cTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAg                     >  2:74702/1‑149 (MQ=255)                                                                                                                              ttGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAgg                    >  1:132049/1‑149 (MQ=255)                                                                                                                                 tgtCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTgag                                                                                            >  7:253319/1‑74 (MQ=255)                                                                                                                                 tgtCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTgag                                                                                            <  8:253319/74‑1 (MQ=255)                                                                                                                                   tCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATg               <  6:226591/149‑1 (MQ=255)                                                                                                                                   tCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATg               >  6:162353/1‑149 (MQ=255)                                                                                                                                    gatcctggggCTGCTGCGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGt              >  4:45014/8‑149 (MQ=255)                                                                                                                                      cgATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGTCa            <  3:43038/149‑1 (MQ=255)                                                                                                                                        ctggggCTGCTGCGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCaa                                                           <  4:233085/97‑1 (MQ=255)                                                                                                                                        ctggggCTGCTGCGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCaa                                                           >  3:233085/4‑100 (MQ=255)                                                                                                                                         tggggCTGCTGCGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATgcgc                                                                                    >  6:307111/3‑74 (MQ=38)                                                                                                                                         tggggCTGCTGCGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATgcgc                                                                                    <  5:307111/72‑1 (MQ=38)                                                                                                                                            ggCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGTCAGTATCg      >  7:239414/1‑149 (MQ=255)                                                                                                                                            ggCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGTCAGTATCg      >  2:86374/1‑149 (MQ=255)                                                                                                                                                gcgcAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGTCAGTATCGCCaa  >  5:228004/1‑149 (MQ=255)                                                                                                                                                 cgcAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGTCAGTATCGCCaa  <  3:287663/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GCACTGGACCCCGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGTCAGTATCGCCAA  >  NC_002947/4971809‑4972100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑