Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,971,956 A→C 12.9% intergenic (‑391/+26) PP_4379 ← / ← flgL 3‑oxoacyl‑ACP synthase/flagellar hook‑associated protein FlgL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,971,9560AC12.9% 146.4 / 10.9 53intergenic (‑391/+26)PP_4379/flgL3‑oxoacyl‑ACP synthase/flagellar hook‑associated protein FlgL
Reads supporting (aligned to +/- strand):  ref base A (27/19);  new base C (3/4);  total (30/23)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACTGGACCCCGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGTCAGTATCGCCAATGG  >  NC_002947/4971809‑4972103
                                                                                                                                                   |                                                                                                                                                   
gcACTGGACCCCGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGAAAGGGCTGCgcag                                                                                                                                                    >  1:109156/1‑149 (MQ=255)
  aCTGGACCCCGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCgcagca                                                                                                                                                  <  2:109156/149‑1 (MQ=255)
  aCTGGACCCCGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCAT‑‑CGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCagcagc                                                                                                                                                <  3:287497/149‑1 (MQ=255)
       aCCCCGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCTGCGCAGccct                                                                                                                                             <  3:278548/149‑2 (MQ=255)
       aCCCCGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGcccc                                                                                                                                             <  4:2332/149‑1 (MQ=255)
          ccGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCTGCGCAGccctatc                                                                                                                                          <  5:242291/149‑5 (MQ=255)
             aaTGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATc                                                                                                                                       <  1:310064/149‑1 (MQ=255)
              aTGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATca                                                                                                                                      <  3:190259/149‑1 (MQ=255)
                 aTCAGCTTCGCAGCAGATGTCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATcagg                                                                                                                                    <  6:40507/148‑1 (MQ=255)
                 aTCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATcagg                                                                                                                                    >  5:226591/1‑148 (MQ=255)
                     gCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATcaggcagg                                                                                                                                >  1:73312/1‑148 (MQ=255)
                            agcagATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGAt                                                                                                                           >  2:29372/1‑146 (MQ=255)
                             gcagATATCGATGGGGCCGCATCGCGGCCCTTTCGCCACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGATGACTACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGa                                                                                                                       <  5:306014/149‑1 (MQ=255)
                                    tCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTc                                                                                                                <  1:143363/149‑1 (MQ=255)
                                      gaaagggCCGCGATGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCg                                                                                                               >  8:134063/5‑148 (MQ=255)
                                         ggggCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGaa                                                                                                             >  1:41956/1‑147 (MQ=255)
                                          gggCCGCATCGCGGCCCTTTCGAGACGCAAGGACGCTCCCAAGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCGTGTGTCGCGATAGGCCTGCGCAGCAGCCCCAGCATCAGGAAGGCAGATTAGATCTTGTCGAACAg                                                                                                          >  5:31648/1‑149 (MQ=255)
                                           ggCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATGAGGCAGGCAGATTAGATCTTGTCGAACAgg                                                                                                         <  1:29372/149‑1 (MQ=255)
                                                 aTCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAGTACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGc                                                                                                   >  4:43038/1‑149 (MQ=255)
                                                  tCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCt                                                                                                  >  6:237126/1‑149 (MQ=255)
                                                  tCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCt                                                                                                  <  4:125877/149‑1 (MQ=255)
                                                             tCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATgc                                                                                         <  7:134063/147‑1 (MQ=255)
                                                               gcgACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACa                                                                                                           >  3:147556/1‑127 (MQ=255)
                                                               gcgACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACa                                                                                                           <  4:147556/127‑1 (MQ=255)
                                                                 gACGCAAGGCCTCTCCCACCAATACGCCACCTCCAGGAGAGAGGACCACCTGTGGGGGCCTTGTGTCGCGATCGGGCTGCGCAGCAGCCCCAGCATCAGCCAGGCAGAATAGAGCTAGTCCACAACGCATAGCTGAGAAATGCGCGCa                                                                                    >  4:237835/1‑148 (MQ=255)
                                                                    gCAAGGCGGCTCCCACGAATACGCCACCTCCATGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAAc                                                                                 <  3:237835/148‑1 (MQ=255)
                                                                      aaGGCCGCTCCCACGATTACGCCACCTACAGGAGAGAGGACGACCTGTGGTGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGc                                                                               >  7:237640/1‑148 (MQ=255)
                                                                      aaGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCCATAGGGCTGCGCAGCAGCCGCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGc                                                                               >  7:242076/1‑148 (MQ=255)
                                                                          ccGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGtt                                                                          >  6:245148/1‑149 (MQ=255)
                                                                              tCCCACGAATACGCCACCTCCAGGAGCGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAAGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCg                                                                       >  1:168937/1‑148 (MQ=255)
                                                                                ccACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCa                                                                             <  2:79361/140‑1 (MQ=255)
                                                                                ccACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCa                                                                             >  1:79361/1‑140 (MQ=255)
                                                                                ccACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGaa                                                                     <  5:245148/148‑1 (MQ=255)
                                                                                      aTACGCCACCTCCAGGAGAGAGGTCGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGATCAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTc                                                                >  7:289960/1‑147 (MQ=255)
                                                                                                      agagagGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAg                                              >  1:184214/1‑149 (MQ=255)
                                                                                                       gagagGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAgg                                             <  5:237126/149‑1 (MQ=255)
                                                                                                         gagGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGt                                           >  8:146626/1‑149 (MQ=255)
                                                                                                         gagGACGACCTGGGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGt                                           <  2:41956/149‑1 (MQ=255)
                                                                                                               gacCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCt                                      >  4:131822/1‑148 (MQ=255)
                                                                                                                 ccTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATTCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGaa                                   >  8:170581/1‑149 (MQ=255)
                                                                                                                            cTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAg                        >  2:74702/1‑149 (MQ=255)
                                                                                                                             ttGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAgg                       >  1:132049/1‑149 (MQ=255)
                                                                                                                                tgtCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTgag                                                                                               >  7:253319/1‑74 (MQ=255)
                                                                                                                                tgtCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTgag                                                                                               <  8:253319/74‑1 (MQ=255)
                                                                                                                                  tCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATg                  <  6:226591/149‑1 (MQ=255)
                                                                                                                                  tCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATg                  >  6:162353/1‑149 (MQ=255)
                                                                                                                                   gatcctggggCTGCTGCGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGt                 >  4:45014/8‑149 (MQ=255)
                                                                                                                                     cgATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGTCa               <  3:43038/149‑1 (MQ=255)
                                                                                                                                       ctggggCTGCTGCGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCaa                                                              <  4:233085/97‑1 (MQ=255)
                                                                                                                                       ctggggCTGCTGCGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCaa                                                              >  3:233085/4‑100 (MQ=255)
                                                                                                                                        tggggCTGCTGCGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATgcgc                                                                                       >  6:307111/3‑74 (MQ=38)
                                                                                                                                        tggggCTGCTGCGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATgcgc                                                                                       <  5:307111/72‑1 (MQ=38)
                                                                                                                                           ggCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGTCAGTATCg         >  7:239414/1‑149 (MQ=255)
                                                                                                                                           ggCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGTCAGTATCg         >  2:86374/1‑149 (MQ=255)
                                                                                                                                               gcgcAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGTCAGTATCGCCaa     >  5:228004/1‑149 (MQ=255)
                                                                                                                                                cgcAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGTCAGTATCGCCaa     <  3:287663/148‑1 (MQ=255)
                                                                                                                                                   agcagcCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGAATGGCCTGATGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGTCAGTATCGCCAATgg  >  5:54143/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCACTGGACCCCGAATGATCAGCTTCGCAGCAGATATCGATGGGGCCGCATCGCGGCCCTTTCGCGACGCAAGGCCGCTCCCACGAATACGCCACCTCCAGGAGAGAGGACGACCTGTGGGGGCCTTGTGTCGCGATAGGGCTGCGCAGCAGCCCCAGGATCAGGCAGGCAGATTAGATCTTGTCGAACAGGCTTAGCTGAGAAATGCGCGCAAACGCCAGTTGCGAAGCTTCAAGCATGGCCTGCTGCAGGGTCAGCTGAATGGTGGCCGAGGCCATGTCAGTATCGCCAATGG  >  NC_002947/4971809‑4972103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑