Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,979,788 T→A 7.0% D141V (GAC→GTC)  flgF ← flagellar basal‑body rod protein FlgF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,979,7880TA 7.0% 161.1 / 2.9 57D141V (GAC→GTC) flgFflagellar basal‑body rod protein FlgF
Reads supporting (aligned to +/- strand):  ref base T (24/29);  new base A (2/2);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01

GCATCGGCCGGCTGGCCGGAAGTGGTATGGATCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCATCGATGTTCAGGCTACCGGTACGCACATAGGCTTCGCTGCCGTCCGGC  >  NC_002947/4979641‑4979934
                                                                                                                                                   |                                                                                                                                                  
gCATCGGCCGGCTGGCCGGAAGTGGTATGGATCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTc                                                                                                                                                   <  2:250663/149‑1 (MQ=255)
  aTCGGCCGGCTGGCCGGAAGTGGTATGGATCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGACgg                                                                                                                                                 >  1:139629/1‑149 (MQ=255)
     ggccggcTGGCCGGAAGTGGTATGGATCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGACGGCa                                                                                                                                               <  2:139629/148‑1 (MQ=255)
     ggccggcTGGCCGGAAGTGGTATGGATCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGA‑CAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCAc                                                                                                                                              <  8:245249/148‑1 (MQ=255)
      gccggcTGGCCGGAAGTGGTATGGATCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCAcc                                                                                                                                             <  1:132225/149‑1 (MQ=255)
       ccggcTGGCCGGAAGTGGTATGGATCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCAccc                                                                                                                                            <  7:264123/149‑1 (MQ=255)
        cggcTGGCCGGAAGTGGTATGGATCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGACGGCAccat                                                                                                                                           <  6:53325/149‑3 (MQ=255)
         ggcTGGCCGGAAGTGGTATGGATCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACccac                                                                                                                                          >  5:209715/1‑149 (MQ=255)
               ccGGAAGTGGTATGGATCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACccacttccac                                                                                                                                    <  6:209715/149‑1 (MQ=255)
                   aaGTGGTATGGATCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTc                                                                                                                                <  7:90746/149‑1 (MQ=255)
                    aGTGGTATGGATCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCt                                                                                                                               <  2:188677/149‑1 (MQ=255)
                        gTATGGATCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTc                                                                                                                           >  6:272749/1‑149 (MQ=255)
                              aTCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGt                                                                                                                                                    <  4:158965/118‑1 (MQ=255)
                              aTCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGt                                                                                                                                                    >  3:158965/1‑118 (MQ=255)
                                  gACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATg                                                                                                                 >  5:191594/1‑149 (MQ=255)
                                   aCCGTCCAGGCCCTTCGTCGGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATgg                                                                                                                >  3:101275/1‑149 (MQ=255)
                                   aCCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATgg                                                                                                                >  1:268913/1‑149 (MQ=255)
                                    ccGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGc                                                                                                               <  2:268913/149‑1 (MQ=255)
                                          aGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCgg                                                                                                         >  3:122320/1‑149 (MQ=255)
                                             cccTTCGTCAGGCCTTGGGTGTCCTGGGTGACCAGTTTGATGCGGTCGCCCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGcc                                                                                                      <  6:169927/149‑1 (MQ=255)
                                                    tCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGGCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTg                                                                                               <  7:188285/149‑1 (MQ=255)
                                                      aGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGc                                                                                              <  2:34573/148‑1 (MQ=255)
                                                        gCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGa                                                                                           >  4:45210/1‑149 (MQ=255)
                                                        gCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGa                                                                                           >  5:214374/1‑149 (MQ=255)
                                                          cTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGc                                                                                              <  6:44557/144‑1 (MQ=255)
                                                          cTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGc                                                                                              >  5:44557/1‑144 (MQ=255)
                                                              ggTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCAcc                                                                                      <  4:122320/148‑1 (MQ=255)
                                                                  tCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCAt                                                                                 <  4:101275/149‑1 (MQ=255)
                                                                            ccAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATg                                                                                                                 <  6:325921/107‑1 (MQ=255)
                                                                            ccAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATg                                                                                                                 >  5:325921/1‑107 (MQ=255)
                                                                            ccAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCg                                                                       <  5:272749/149‑1 (MQ=255)
                                                                                   gATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACg                                                                  >  6:155994/1‑147 (MQ=255)
                                                                                       cGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACgcagca                                                             <  3:45210/148‑1 (MQ=255)
                                                                                         gTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCacac                                                          >  1:131044/1‑149 (MQ=255)
                                                                                             aCCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAg                                                      >  5:277527/1‑149 (MQ=255)
                                                                                             aCCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAg                                                      <  6:191594/149‑1 (MQ=255)
                                                                                              ccTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAgg                                                     <  7:241294/149‑1 (MQ=255)
                                                                                              acccGGACACCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAgg                                                     <  6:166822/146‑1 (MQ=255)
                                                                                                 cGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCa                                                  >  6:50852/1‑149 (MQ=255)
                                                                                                     cATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGAAGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCATcca                                              >  7:69364/1‑147 (MQ=255)
                                                                                                      aTCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGAACGCAGCACACCGAGGGCATCGAt                                             >  4:171768/1‑149 (MQ=255)
                                                                                                        cACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCAACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCATCGATGt                                           >  1:77850/1‑149 (MQ=255)
                                                                                                              cgGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCATCGATGTTCAGGc                                     <  6:277527/149‑1 (MQ=255)
                                                                                                               gggTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATc                                                                                                           >  8:155553/1‑78 (MQ=255)
                                                                                                               gggTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATc                                                                                                           <  7:155553/78‑1 (MQ=255)
                                                                                                                ggTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCATCGATGTTCAGGCTa                                   <  6:214374/149‑1 (MQ=255)
                                                                                                                  tCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCATCGATGTTCAGGCTAcc                                 <  3:255391/149‑1 (MQ=255)
                                                                                                                    ttCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCATCGATGTTCAGGCTACCgg                               >  2:269649/1‑149 (MQ=255)
                                                                                                                       aCCCATGGGGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCGCGCAGCACACCGAGGGCATCGATGTTCAGGCTACCGGTAc                            >  1:12700/1‑149 (MQ=255)
                                                                                                                       aCCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCATCGATGTTCAGGCTACCGGTAc                            <  5:50852/149‑1 (MQ=255)
                                                                                                                         ccATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCATCGATGTTCAGGCTACCGGTACg                           >  6:314671/1‑148 (MQ=255)
                                                                                                                              gAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCATCGATGTTCAGGCTACCGGTACGCACAt                      <  1:307343/148‑1 (MQ=255)
                                                                                                                                      gCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCATCGATGTTCAGGCTACCGGTACGCACATAGGCTTCGc             >  5:279557/1‑149 (MQ=255)
                                                                                                                                       cTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCATCGATGTTCAGGCTACCGGTACGCACATAGGCTTCGc             <  5:314671/148‑1 (MQ=255)
                                                                                                                                        tGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCATCGATGTTCAGGCTACCGGTACGCACATAGGCTTCGCTg           >  2:209278/1‑149 (MQ=255)
                                                                                                                                         gtgggTGCCGACGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCATCGATGTTCAGGCTACCGGTACGCACATAGGCTTCGCTGc          >  3:858/4‑149 (MQ=255)
                                                                                                                                                ccGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAgg                                                     <  7:23773/99‑1 (MQ=255)
                                                                                                                                                ccGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAgg                                                     >  8:23773/1‑99 (MQ=255)
                                                                                                                                                 cGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCTCGCAGCACACCGAGGGCATCGATGTTCAGGCTACCGGTACGCACATAGGCTTCGCTGCCGTCCGgc  <  2:12700/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCATCGGCCGGCTGGCCGGAAGTGGTATGGATCAGACCGTCCAGGCCCTTCGTCAGGCCTTTGGTGTCCGGGTTGACCAGTTTGATGCGGTCGACCTCGGCCATCACCCGCGGGTCTTCACCCATGGAGCGGATGCTGATGGTGCCGTCGGCACCCACTTCCACTTTCTGCTCCGGCGGGATGGCGATCGGGCCACCGTTGCCGATCACCGGCATGCCATTGCCGGCACGCAGCACACCGAGGGCATCGATGTTCAGGCTACCGGTACGCACATAGGCTTCGCTGCCGTCCGGC  >  NC_002947/4979641‑4979934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑