Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,988,365 G→C 7.3% intergenic (+35/+59) ycgR → / ← PP_4398 flagellar brake protein YcgR/MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,988,3650GC 7.3% 152.1 / 2.3 51intergenic (+35/+59)ycgR/PP_4398flagellar brake protein YcgR/MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (18/29);  new base C (2/2);  total (20/31)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGGCGTGCGTTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCGGTGTCTTCAGCCGCCACCGGCGCCTG  >  NC_002947/4988223‑4988510
                                                                                                                                              |                                                                                                                                                 
gCCGGCGTGCGTTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTg                                                                                                                                              <  6:191173/148‑1 (MQ=255)
 ccGGCGTGCGTTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGc                                                                                                                                            <  3:59605/149‑1 (MQ=255)
 ccGGCGTGCGTTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGc                                                                                                                                            >  6:115373/1‑149 (MQ=255)
    gcgtgcgtTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGtt                                                                                                                                         <  6:90072/149‑1 (MQ=255)
     cgtgcgtTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGGGGCGttt                                                                                                                                        <  5:166023/149‑1 (MQ=255)
       tgcgtTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTCAGCAGGTGGCGTTTTc                                                                                                                                      <  8:191911/149‑1 (MQ=255)
        gcgtTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCa                                                                                                                                     >  6:189540/1‑149 (MQ=255)
         cgtTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCa                                                                                                                                     <  8:212430/148‑1 (MQ=255)
           tttCCACAACCTGAGCGGGTAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCAttt                                                                                                                                  <  5:28893/149‑1 (MQ=255)
           tttCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCAttt                                                                                                                                  >  7:40421/1‑149 (MQ=255)
              ccACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTtgt                                                                                                                                <  5:189540/148‑1 (MQ=255)
                    ccTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTctg                                                                                                                         >  3:62080/1‑149 (MQ=255)
                      tGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTtgt                                                                                                                                <  4:69363/140‑1 (MQ=255)
                      tGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTtgt                                                                                                                                >  3:69363/1‑140 (MQ=255)
                         gCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTgctggg                                                                                                                    <  1:36065/149‑1 (MQ=255)
                         gCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTgctggg                                                                                                                    <  5:115373/149‑1 (MQ=255)
                                      gcgcaAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTcgc                                                                                                       <  5:221820/149‑1 (MQ=255)
                                      gcgcaAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTcgc                                                                                                       <  8:40421/149‑1 (MQ=255)
                                        gcaAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGcgaagcgg                                                                                                     >  3:296462/1‑142 (MQ=255)
                                             aTCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGc                                                                                                                                            >  7:93707/1‑105 (MQ=255)
                                             aTCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGc                                                                                                                                            <  8:93707/105‑1 (MQ=255)
                                                gagaGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAAccc                                                                                             >  3:96659/1‑149 (MQ=255)
                                                 agagTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCgg                                                                                                     <  6:28298/140‑1 (MQ=255)
                                                 agagTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCgg                                                                                                     >  5:28298/1‑140 (MQ=255)
                                                 agagTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCg                                                                                            <  7:23433/149‑1 (MQ=255)
                                                 agagTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCg                                                                                            >  2:259309/1‑149 (MQ=255)
                                                  gagTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGc                                                                                           <  6:307872/149‑1 (MQ=255)
                                                    gTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTc                                                                                         <  1:293781/149‑1 (MQ=255)
                                                     tttCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTcc                                                                                        >  1:202412/1‑149 (MQ=255)
                                                               cAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGtttt                                                                                                                                       <  4:302036/92‑1 (MQ=255)
                                                               cAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGtttt                                                                                                                                       >  3:302036/1‑92 (MQ=255)
                                                                    gCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCt                                                                          >  3:188351/1‑148 (MQ=255)
                                                                      aGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGAc                                                                       <  4:62080/149‑1 (MQ=255)
                                                                      aGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGAc                                                                       <  6:88152/149‑1 (MQ=255)
                                                                              gcgcGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAg                                                               <  1:259309/149‑1 (MQ=255)
                                                                                    cggTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCt                                                         <  2:62765/149‑1 (MQ=255)
                                                                                             aaGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTgcg                                                >  1:182375/1‑149 (MQ=255)
                                                                                                gACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTgcgcgc                                             <  7:74956/149‑1 (MQ=255)
                                                                                                 aCGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGcc                                            <  2:86055/149‑1 (MQ=255)
                                                                                                        ttGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTctct                                     <  2:102572/149‑1 (MQ=255)
                                                                                                           aTCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGAc                                                                               >  3:57630/1‑104 (MQ=255)
                                                                                                           aTCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGAc                                                                               <  4:57630/104‑1 (MQ=255)
                                                                                                               tcggtcggTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCt                              >  8:217325/1‑149 (MQ=255)
                                                                                                                 ggtcggtAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCg                            <  2:202412/149‑1 (MQ=255)
                                                                                                                  gtcggtAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTGTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCATGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCgg                           <  4:96659/149‑1 (MQ=255)
                                                                                                                      gtAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCGGTGTc                       <  2:182375/149‑1 (MQ=255)
                                                                                                                          aaatgAAAACGCCACCTCAGCAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCGGTGTCtt                     >  5:60954/5‑147 (MQ=255)
                                                                                                                               aaaaCGCCACCTCAGCAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCGGTGTCTTCa                   <  3:27306/144‑1 (MQ=255)
                                                                                                                               aaaaCGCCACCTCAGCAGGTGGCGTTTTCATGTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGGAAACCCGCTCCTAGACCGGCTTGCTGGCCTCTGCAGGCGCCGGATGCTGCGCGCCCTTGGCTGCAGCGTCGGTGTGTTCAgagag              >  4:27306/1‑145 (MQ=255)
                                                                                                                                 aaCGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCGGTGTCTTCAGCCGCCa            >  1:244855/1‑149 (MQ=255)
                                                                                                                                    gCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCGGTGTCTTCAGCCGCCACCg         >  8:175665/1‑149 (MQ=255)
                                                                                                                                         ctgctgAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGt                                                       <  4:206202/98‑1 (MQ=255)
                                                                                                                                         ctgctgAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGt                                                       >  3:206202/1‑98 (MQ=255)
                                                                                                                                         ctgctgAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCGGTGTCTTCAGCCGCCACCGGCGcc    >  4:201006/1‑149 (MQ=255)
                                                                                                                                           gctgAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCGGTGTCTTCAGCCGCCACCGGCGCCTg  >  7:43802/1‑149 (MQ=255)
                                                                                                                                            ctgAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGAGTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCGGTGTCTTCAGCCTCCACCGGCGCCTg  >  3:21605/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GCCGGCGTGCGTTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGACGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCGGTGTCTTCAGCCGCCACCGGCGCCTG  >  NC_002947/4988223‑4988510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑