Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,074,670 T→C 9.0% intergenic (‑107/‑75) PP_5692 ← / → PP_4468 hypothetical protein/Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,074,6700TC 9.0% 151.6 / 4.8 57intergenic (‑107/‑75)PP_5692/PP_4468hypothetical protein/Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (29/22);  new base C (2/3);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

GCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGCGGGCACACTTCGAGCCCTACGGCTAAACCGTCGCTTTCGGTAC  >  NC_002947/5074528‑5074810
                                                                                                                                              |                                                                                                                                             
gcgGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACg                                                                                                                                          >  8:100899/1‑148 (MQ=255)
        tCAACAGGCCATGCTCAATGGGATTCACGCCCTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGa                                                                                                                                        >  1:51560/1‑142 (MQ=255)
        tCAACAGGCCATGCTCAATGGGATTCACGCCCTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGa                                                                                                                                        <  2:51560/142‑1 (MQ=255)
          aaCAGGCCATGCTCAATGGGATTCACGCCCTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCt                                                                                                                                >  2:272169/1‑148 (MQ=255)
          aaCAGGCCATGCTCAATGGGATTCACGCCCTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGACGGACGGATAATAcc                                                                                                                                 <  7:155420/147‑1 (MQ=255)
              ggCCATGCTCAATGGGATTCACGCCCTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGCCATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCAttt                                                                                                                           >  2:15014/1‑149 (MQ=255)
               gCCATGCTCAATGGGATTCACGCCCTTTTCCCCGCGCTCAGAAATATTTGAGAATTTCCGACATCACCGCATTAATGCCACTTTTGTAGGCTTCAAACATTGAACAGTCTGAACGTTTTCTCCTAGATGGACGGATAATACATAAttt                                                                                                                           >  2:247756/1‑148 (MQ=255)
                ccATGCTCAATGTGATTCACGCCCTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGa                                                                                                                         <  3:214407/149‑1 (MQ=255)
                 cATGCTCAATGGGATTCACGCCCTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGACGGACGGATAATACCTCATTTGAg                                                                                                                        >  3:272393/1‑149 (MQ=255)
                 cATGCTCAATGGGATTCACGCCCTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGACGGACGGATAATACCTCATTTGAg                                                                                                                        >  7:145431/1‑149 (MQ=255)
                        aaTGGGATTCACGGCCTTTTGCCCGGGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCGCTTTTGTAGGCTTGAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCAt                                                                                                                             <  7:273535/137‑1 (MQ=255)
                              aTTCACGCCCTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGaaa                                                                                                           <  1:103204/149‑1 (MQ=255)
                                  aCGCCCTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCt                                                                                                       >  8:186934/1‑149 (MQ=255)
                                  aCGCCCTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCt                                                                                                       >  8:163202/1‑149 (MQ=255)
                                  aCGCCCTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCt                                                                                                       >  1:275924/1‑149 (MQ=255)
                                  aCGCCCATTACCCCCAGCTCAGCAATATTTGAGAATTTCCGACATAACCGCCTTAATGCCCATTTTGTAGGCTTCAAACCTTGAACAGTCTTAACATTATCCACTAGATGGACTCAAAATACCTCATTTCAGTGTTTATCCGAAAggat                                                                                                       >  2:307075/1‑147 (MQ=255)
                                      ccTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACTGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCtg                                                                                                   >  3:96637/1‑149 (MQ=255)
                                            ccccGCGCTCAGCAATATTTGAGAATTTCCGACATCACCTCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGACGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTgg                                                                                              <  4:272393/148‑1 (MQ=255)
                                            ccccGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGACGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTgg                                                                                              <  8:145431/148‑1 (MQ=255)
                                             cccGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCt                                                                                            <  7:100899/149‑1 (MQ=255)
                                              ccGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCta                                                                                           >  5:289211/1‑149 (MQ=255)
                                                      aGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGa                                                                                  >  2:264195/1‑149 (MQ=255)
                                                         aaTATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACtt                                                                               >  6:275240/1‑149 (MQ=255)
                                                           tatTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACtttt                                                                             <  7:186934/149‑1 (MQ=255)
                                                           tatTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTATGTAGGCTTCAAACCTATAACAGTCTGAACCTTTTCGCCTAGAAGGACGGATAATACATCATTAGACTGTTTATACGAAAGGCTTCTCTGAGGCTATATC‑CTGACattt                                                                             >  3:29663/1‑149 (MQ=255)
                                                             tttGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACTGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTg                                                                            <  4:96637/148‑1 (MQ=255)
                                                                  gaATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCaaa                                                                      <  2:275924/149‑1 (MQ=255)
                                                                     tttCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCa                                                                   <  1:272169/149‑1 (MQ=255)
                                                                       tCCGAAATCACCGACTTAATGCACCTTTTGTATGCTTCAAACCTTGAAGAGTCTGAAGGTTTTTTCCTAGATGTACTGATAATACCTCATTTGTGTGTTTATGCGAAAGGCTTCTGTGTGGCTATATC‑GTGACTTTTGTCCATACCAt                                                                  <  1:307075/148‑1 (MQ=255)
                                                                         cGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGa                                                                                                                                        >  2:293486/1‑77 (MQ=255)
                                                                         cGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGa                                                                                                                                        <  1:293486/77‑1 (MQ=255)
                                                                         cGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATg                                                                 >  3:62365/1‑147 (MQ=255)
                                                                           aCATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGc                                                             >  3:81689/1‑149 (MQ=255)
                                                                              tCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTga                                                          <  6:289211/149‑1 (MQ=255)
                                                                              tCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATCCAGCt                                                            >  2:127862/1‑147 (MQ=255)
                                                                               cACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGa                                                                                                                         <  7:174341/86‑1 (MQ=255)
                                                                               cACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGa                                                                                                                         >  8:174341/1‑86 (MQ=255)
                                                                                aCCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCt                                                                                                    >  7:38097/1‑106 (MQ=255)
                                                                                aCCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCt                                                                                                    <  8:38097/106‑1 (MQ=255)
                                                                                aCCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCCAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTg                                                                            >  7:6820/1‑129 (MQ=255)
                                                                                aCCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCCAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTg                                                                            <  8:6820/129‑1 (MQ=255)
                                                                                    cattAATTCCGCTTTTGTAGGCTTGAATCCTTGAACAGTCTGAACGTTTTCGCCTTGATGGTCGGATAATACGTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGATTTg                                                    <  4:29663/147‑1 (MQ=255)
                                                                                        aaTGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATA‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGccc                                                 >  4:289225/1‑148 (MQ=255)
                                                                                           gCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGc                                             >  3:47370/1‑149 (MQ=255)
                                                                                           gCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGc                                             >  2:78264/1‑149 (MQ=255)
                                                                                             cccTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGCgg                                           <  1:15014/149‑1 (MQ=255)
                                                                                             cccTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGCgg                                           >  2:62238/1‑149 (MQ=255)
                                                                                                       ggCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATCTCTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGCGGGCACACTTc                                  <  1:78264/149‑1 (MQ=255)
                                                                                                          ttCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGCGGGCACACTTCGAGc                              >  6:246828/1‑149 (MQ=255)
                                                                                                                      aCAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGCGGGCACACTTCGAGcc                             <  4:6529/138‑1 (MQ=255)
                                                                                                                      aCAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGCGGGCACACTTCGAGcc                             >  3:6529/1‑138 (MQ=255)
                                                                                                                      aCAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGCGGGCACACTTCGAGCCCTACGGCTaaa                  <  1:264195/149‑1 (MQ=255)
                                                                                                                              aaCGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGCGGGCACACTTCGAGCCCTACGGCTAAACCGTc             <  7:163202/146‑1 (MQ=255)
                                                                                                                                 gTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGCGGGCACACTTCGAGCCCTACGGCTAAACCGTCGCTTTc       >  5:56621/1‑149 (MQ=255)
                                                                                                                                   tttCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGCGGGCACACTTCGAGCCCTACGGCTAAACCGTCGCTTTCgg     >  4:295457/1‑149 (MQ=255)
                                                                                                                                       gCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGCGGGCACACTTCGAGCCCTACGGCTAAACCGTCGCTTTCGGTAc  <  4:47370/148‑1 (MQ=255)
                                                                                                                                       gCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGCGGGCACACTTCGAGCCCTACGGCTAAACCGTCGCTTTCGGTAc  >  4:260346/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                             
GCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTTTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGATAATACCTCATTTGAGTGTTTATCCGAAAGGCTTCTGTGTGGCTATATC‑CTGACTTTTGGCCAAACCATGCAGCTGAGAATTGCCCTAGCGGGCACACTTCGAGCCCTACGGCTAAACCGTCGCTTTCGGTAC  >  NC_002947/5074528‑5074810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑