Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,119,195 G→C 9.9% L323L (CTG→CTC)  PP_4505 → sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,119,1950GC 9.9% 169.1 / 8.1 59L323L (CTG→CTC) PP_4505sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (29/24);  new base C (3/3);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.82e-01

ACCGCCTGGAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCGTGAT  >  NC_002947/5119047‑5119341
                                                                                                                                                    |                                                                                                                                                  
aCCGCCTGGAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTg                                                                                                                                                    <  4:209357/149‑1 (MQ=255)
aCCGCCTGGAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTg                                                                                                                                                    <  3:161864/149‑1 (MQ=255)
   gCCTGGAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAg                                                                                                                                                 <  6:278900/149‑1 (MQ=255)
            aCCTGATCCGCGAAATCCTCGCCGTGGCCCGGGTGGATGCCTAACAAGCCCATGCCGACCCGGTCTACCTCAATCCCCTACTCGGCAGCGGGCGAAAGGACGCCCTGCTGAGCGCGCCGGATCAGTAGGTGCGCCTCCAGGCGCacgtc                                                                                                                                        <  6:38256/149‑5 (MQ=255)
              cTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAgg                                                                                                                                      >  1:311066/1‑149 (MQ=255)
                      cGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCt                                                                                                                              >  2:76935/1‑149 (MQ=255)
                      cGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCACGTTTGAGCt                                                                                                                              >  5:323386/1‑149 (MQ=255)
                        aaaTCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTg                                                                                                                            >  6:190361/1‑149 (MQ=255)
                           tCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAg                                                                                                                         <  3:304740/149‑1 (MQ=255)
                             cTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAgg                                                                                                                        <  6:149073/148‑1 (MQ=255)
                             cTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGCACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAggg                                                                                                                       >  7:128744/1‑149 (MQ=255)
                                gCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGtt                                                                                                                     <  8:238486/148‑1 (MQ=255)
                                      gCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGtt                                                                                                                                    >  8:77576/1‑127 (MQ=255)
                                      gCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGtt                                                                                                                                    <  7:77576/127‑1 (MQ=255)
                                       cccGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACg                                                                                                             >  4:176854/1‑149 (MQ=255)
                                               gATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGa                                                                                                                                 >  6:246777/1‑121 (MQ=255)
                                                       aCAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCg                                                                                             >  7:18640/1‑149 (MQ=255)
                                                         aaGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGtt                                                                                           >  8:88638/1‑149 (MQ=255)
                                                             ccATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGAc                                                                                        >  2:264124/1‑148 (MQ=255)
                                                               aTGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAAc                                                                                     >  4:286337/1‑149 (MQ=255)
                                                                   cGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACctgc                                                                                 >  6:46339/1‑149 (MQ=255)
                                                                    gAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACctgct                                                                                >  3:133538/1‑149 (MQ=255)
                                                                          gTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCaa                                                                          >  8:65490/1‑149 (MQ=255)
                                                                          gTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCaa                                                                          >  4:266719/1‑149 (MQ=255)
                                                                                cTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCt                                                                    <  2:311066/149‑1 (MQ=255)
                                                                                     tGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCt                                                               <  7:88638/149‑1 (MQ=255)
                                                                                      gCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGATCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCtt                                                              >  2:70931/1‑149 (MQ=255)
                                                                                          tACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAAc                                                          >  3:85170/1‑149 (MQ=255)
                                                                                          tACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAAc                                                          >  5:326225/1‑149 (MQ=255)
                                                                                             tCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCcg                                                       >  7:146026/1‑149 (MQ=255)
                                                                                             tCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGA‑AACCTGCTGCGCAATGCCCTGCGCTTCAACCcgg                                                      >  7:244892/1‑149 (MQ=255)
                                                                                                   gCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGc                                                 <  8:146026/149‑1 (MQ=255)
                                                                                                         gcAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGcc                                                >  8:152024/1‑144 (MQ=255)
                                                                                                         gcAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGcc                                                <  7:152024/144‑1 (MQ=255)
                                                                                                         gcAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCg                                           >  8:23721/1‑149 (MQ=255)
                                                                                                              gACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGa                                      <  7:65490/149‑1 (MQ=255)
                                                                                                               aCGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAg                                     <  3:286337/149‑1 (MQ=255)
                                                                                                               aCGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAg                                     <  8:128744/149‑1 (MQ=255)
                                                                                                               aCGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAg                                     <  1:76935/149‑1 (MQ=255)
                                                                                                               aCGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAg                                     <  8:18640/149‑1 (MQ=255)
                                                                                                                cGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAgg                                    <  4:21210/149‑1 (MQ=255)
                                                                                                                 gCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGt                                   >  1:185043/1‑149 (MQ=255)
                                                                                                                   ccTGCTGAGCGCGCCGGATCAGGACGTGCGCATCGAGGAGCAGCGACGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCGGTTGACAACATGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGGCAGCCGGTCGAGGt                                   >  7:153000/1‑147 (MQ=255)
                                                                                                                     tgctgAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAgc                               >  6:74902/1‑149 (MQ=255)
                                                                                                                      gctgAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGc                                             >  5:300749/1‑134 (MQ=255)
                                                                                                                      gctgAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGc                                             <  6:300749/134‑1 (MQ=255)
                                                                                                                          aGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGcgcc                            <  6:7505/147‑1 (MQ=255)
                                                                                                                           gcgcgcCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGA‑AACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCcgcgc                        <  8:244892/149‑1 (MQ=255)
                                                                                                                               gcCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCcgcgcgcg                     <  5:46339/149‑1 (MQ=255)
                                                                                                                                ccGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGa                    <  5:290285/149‑1 (MQ=255)
                                                                                                                                 cGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAg                   >  1:196618/1‑149 (MQ=255)
                                                                                                                                 cGGATCAGGACGTGCGCATGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAg                   >  3:141374/1‑149 (MQ=255)
                                                                                                                                   gATCAGGACGTGCGCATGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCa                 <  4:141374/149‑1 (MQ=255)
                                                                                                                                    aTCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAg                <  7:23721/149‑1 (MQ=255)
                                                                                                                                    aTCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAg                <  2:196618/149‑1 (MQ=255)
                                                                                                                                        ctggctGCGCCTCCAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGcc                                                >  5:214931/6‑113 (MQ=255)
                                                                                                                                        ctggctGCGCCTCCAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGcc                                                <  6:214931/108‑1 (MQ=255)
                                                                                                                                               cgcCTCCAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCg                                       <  7:35772/115‑1 (MQ=255)
                                                                                                                                               cgcCTCCAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCg                                       >  8:35772/1‑115 (MQ=255)
                                                                                                                                                gcCTGGAGGCTCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCGTg    <  1:70931/149‑1 (MQ=255)
                                                                                                                                                  cTGGAGGCGCAGCCAGGTTCGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCGTGAt  >  2:295954/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACCGCCTGGAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAACCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAATGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCGTGAT  >  NC_002947/5119047‑5119341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑