Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,149,180	G→C	7.9%	intergenic (‑166/+282)	PP_mr51 ← / ← PP_4535	C4 ncRNA/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,149,180	0	G	C	7.9%	185.9 / 6.7	63	intergenic (‑166/+282)	PP_mr51/PP_4535	C4 ncRNA/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/32);  new base C (3/2);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-01

CAATTTGAGTCGGCCTGTCAAAGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCATTCTGAA  >  NC_002947/5149034‑5149325                                                                                                                                                    |                                                                                                                                                  cAATTTGAGTCGGCCTGTCAAAGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGc                                                                                                                                                 <  4:108466/149‑1 (MQ=255)   aTTTGAGTCGGCCTGTCAAAGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGt                                                                                                                                               <  6:296211/149‑1 (MQ=255)    tttGAGTCGGCCTGTCAAAGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGt                                                                                                                                               >  5:98172/1‑148 (MQ=255)                  tCAAAGCCGTTCGTGGAATGGCAACGACCAAGCGAGACTAGTTGCCAACT‑CGGAACTGCACAATGGGCGACACTATCTTTGGGAAATCTCCTACAAGAAACGGGCAAAATCTGATTTTTTTAGCCGTTACGCCTAACGGctaaaaaa                                                                                                                                  <  7:36330/147‑8 (MQ=255)                  tCAAAGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAAcgc                                                                                                                                <  6:174886/149‑1 (MQ=255)                  tCAAAGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAAcg                                                                                                                                 <  6:207010/148‑1 (MQ=255)                  tCAAAGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAAc                                                                                                                                  <  1:153708/147‑1 (MQ=255)                    aaaGCGGTTCGTTTAAGGGCAACTACCAAGCTAGACTAGTGGCCAACTCCTCGCCTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAAcgc                                                                                                                                <  1:260200/148‑1 (MQ=255)                    aaaGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCAt                                                                                                                                       <  5:147090/140‑1 (MQ=255)                    aaaGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCAt                                                                                                                                       >  6:147090/1‑140 (MQ=255)                     aaGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGca                                                                                                                             >  2:210521/1‑149 (MQ=255)                       gCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGcaca                                                                                                                           >  6:317742/1‑149 (MQ=255)                           gTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTaa                                                                                                                                             >  3:16825/1‑127 (MQ=255)                           gTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTaa                                                                                                                                             <  4:16825/127‑1 (MQ=255)                            tCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCg                                                                                                                      >  8:232997/1‑149 (MQ=255)                               ttGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGa                                                                                                                   <  5:317742/149‑1 (MQ=255)                                 gAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAg                                                                                                                  >  5:1088/1‑148 (MQ=255)                                       cAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAgtg                                                                                                           >  6:135077/1‑149 (MQ=255)                                        aaCGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGTCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAgtgt                                                                                                          >  6:130116/1‑149 (MQ=255)                                          cGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGttt                                                                                                        <  1:210521/149‑1 (MQ=255)                                            aCCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGCCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGAGCACACACCTTGAGT‑AAGTGTTTTTc                                                                                                     >  5:249896/1‑149 (MQ=255)                                             ccAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTc                                                                                                     >  1:291674/1‑149 (MQ=255)                                                 gCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGa                                                                                                                   <  1:141871/131‑1 (MQ=255)                                                 gCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGa                                                                                                                   >  2:141871/1‑131 (MQ=255)                                                       cTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAg                                                                                            <  7:232997/148‑1 (MQ=255)                                                             gCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGcc                                                                                     >  2:70215/1‑149 (MQ=255)                                                             gCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGc                                                                                      <  5:130116/148‑1 (MQ=255)                                                                      ggAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGt                                                                              <  4:66701/148‑1 (MQ=255)                                                                      ggAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGt                                                                              <  6:98172/148‑1 (MQ=255)                                                                             cacaATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAgc                                                                       <  8:113197/148‑1 (MQ=255)                                                                             cacaATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAgc                                                                       <  6:1088/148‑1 (MQ=255)                                                                                aaTGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCa                                                                    >  7:290066/1‑148 (MQ=255)                                                                                              tCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAg                                                     >  7:250738/1‑149 (MQ=255)                                                                                                   gggAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCAc                                                >  1:209181/1‑149 (MQ=255)                                                                                                     gAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCAt                                                                                                                                       >  2:133610/1‑60 (MQ=255)                                                                                                       aaTGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGc                                            <  5:135077/149‑1 (MQ=255)                                                                                                          gTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCtc                                         >  8:113136/1‑148 (MQ=255)                                                                                                             cttcAAGATAGGGGCTAAATGTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGGACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAg                                                     <  6:27753/131‑1 (MQ=255)                                                                                                             cTACACGAAAGGGGCAAACTCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCCCACACCGTGAGT‑AAGTGGTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTaa                                     <  6:249896/149‑1 (MQ=255)                                                                                                             cTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTaaa                                                                                                              >  7:273565/1‑77 (MQ=255)                                                                                                             cTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTaaa                                                                                                              <  8:273565/77‑1 (MQ=255)                                                                                                             cTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGt                                       <  2:209181/148‑1 (MQ=255)                                                                                                             cTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGt                                       >  8:271171/1‑148 (MQ=255)                                                                                                             cTACAAGAAAGGGGCAAAATCTGAATTTTTTAGCCGTTAGGAGTAACGGCATGTAACGCGCACACACCGTGAGTAAACTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTAAATCAAg                                                     >  5:27753/1‑134 (MQ=255)                                                                                                                 aaGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAgg                                                    <  2:113647/131‑1 (MQ=255)                                                                                                                 aaGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAgg                                                    >  1:113647/1‑131 (MQ=255)                                                                                                                  aGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGcc                                           >  6:13381/1‑139 (MQ=255)                                                                                                                  aGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGcc                                           <  5:13381/139‑1 (MQ=255)                                                                                                                        gggCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTCGTAACTATgcgcgc                           <  7:113136/149‑1 (MQ=255)                                                                                                                          gCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATgcgc                             >  3:66592/1‑145 (MQ=255)                                                                                                                          gCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATgcgc                             <  4:66592/145‑1 (MQ=255)                                                                                                                           cAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTc                        <  8:290066/149‑1 (MQ=255)                                                                                                                               aTCTGATTTTTTTAGCCGTTAGGCGTAAGGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATgcgc                             >  1:18630/1‑140 (MQ=255)                                                                                                                               aTCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATgcgc                             <  2:18630/140‑1 (MQ=255)                                                                                                                                          ttAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGc          <  1:70215/148‑1 (MQ=255)                                                                                                                                           atgCCGTTACGCCTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCAt        >  3:278455/3‑149 (MQ=255)                                                                                                                                           atgCCGTTACGCCTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCAt        >  3:144979/3‑149 (MQ=255)                                                                                                                                             gCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCATTc      <  7:271171/149‑1 (MQ=255)                                                                                                                                             gCCGTTACGCCTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCATTc      >  1:212499/1‑149 (MQ=255)                                                                                                                                                gTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCa         >  6:174470/1‑143 (MQ=255)                                                                                                                                                gTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCa         <  5:174470/143‑1 (MQ=255)                                                                                                                                                 ttAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCATTCTGaa  <  8:250738/149‑1 (MQ=255)                                                                                                                                                 ttACGCCTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCATTCTGaa  <  2:212499/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CAATTTGAGTCGGCCTGTCAAAGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCATTCTGAA  >  NC_002947/5149034‑5149325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑