Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,149,183	G→C	8.1%	intergenic (‑169/+279)	PP_mr51 ← / ← PP_4535	C4 ncRNA/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,149,183	0	G	C	8.1%	185.4 / 6.8	62	intergenic (‑169/+279)	PP_mr51/PP_4535	C4 ncRNA/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/31);  new base C (3/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.16e-01

ATTTGAGTCGGCCTGTCAAAGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCATTCTGAA  >  NC_002947/5149036‑5149325                                                                                                                                                     |                                                                                                                                               aTTTGAGTCGGCCTGTCAAAGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGt                                                                                                                                               <  6:296211/149‑1 (MQ=255)  tttGAGTCGGCCTGTCAAAGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGt                                                                                                                                               >  5:98172/1‑148 (MQ=255)                tCAAAGCCGTTCGTGGAATGGCAACGACCAAGCGAGACTAGTTGCCAACT‑CGGAACTGCACAATGGGCGACACTATCTTTGGGAAATCTCCTACAAGAAACGGGCAAAATCTGATTTTTTTAGCCGTTACGCCTAACGGctaaaaaa                                                                                                                                  <  7:36330/147‑8 (MQ=255)                tCAAAGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAAcgc                                                                                                                                <  6:174886/149‑1 (MQ=255)                tCAAAGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAAcg                                                                                                                                 <  6:207010/148‑1 (MQ=255)                tCAAAGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAAc                                                                                                                                  <  1:153708/147‑1 (MQ=255)                  aaaGCGGTTCGTTTAAGGGCAACTACCAAGCTAGACTAGTGGCCAACTCCTCGCCTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAAcgc                                                                                                                                <  1:260200/148‑1 (MQ=255)                  aaaGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCAt                                                                                                                                       <  5:147090/140‑1 (MQ=255)                  aaaGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCAt                                                                                                                                       >  6:147090/1‑140 (MQ=255)                   aaGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGca                                                                                                                             >  2:210521/1‑149 (MQ=255)                     gCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGcaca                                                                                                                           >  6:317742/1‑149 (MQ=255)                         gTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTaa                                                                                                                                             <  4:16825/127‑1 (MQ=255)                         gTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTaa                                                                                                                                             >  3:16825/1‑127 (MQ=255)                          tCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCg                                                                                                                      >  8:232997/1‑149 (MQ=255)                             ttGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGa                                                                                                                   <  5:317742/149‑1 (MQ=255)                               gAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAg                                                                                                                  >  5:1088/1‑148 (MQ=255)                                     cAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAgtg                                                                                                           >  6:135077/1‑149 (MQ=255)                                      aaCGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGTCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAgtgt                                                                                                          >  6:130116/1‑149 (MQ=255)                                        cGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGttt                                                                                                        <  1:210521/149‑1 (MQ=255)                                          aCCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGCCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGAGCACACACCTTGAGT‑AAGTGTTTTTc                                                                                                     >  5:249896/1‑149 (MQ=255)                                           ccAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTc                                                                                                     >  1:291674/1‑149 (MQ=255)                                               gCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGa                                                                                                                   <  1:141871/131‑1 (MQ=255)                                               gCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGa                                                                                                                   >  2:141871/1‑131 (MQ=255)                                                     cTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAg                                                                                            <  7:232997/148‑1 (MQ=255)                                                           gCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGcc                                                                                     >  2:70215/1‑149 (MQ=255)                                                           gCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGc                                                                                      <  5:130116/148‑1 (MQ=255)                                                                    ggAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGt                                                                              <  4:66701/148‑1 (MQ=255)                                                                    ggAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGt                                                                              <  6:98172/148‑1 (MQ=255)                                                                           cacaATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAgc                                                                       <  8:113197/148‑1 (MQ=255)                                                                           cacaATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAgc                                                                       <  6:1088/148‑1 (MQ=255)                                                                              aaTGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCa                                                                    >  7:290066/1‑148 (MQ=255)                                                                                            tCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAg                                                     >  7:250738/1‑149 (MQ=255)                                                                                                 gggAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCAc                                                >  1:209181/1‑149 (MQ=255)                                                                                                   gAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCAt                                                                                                                                       >  2:133610/1‑60 (MQ=255)                                                                                                     aaTGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGc                                            <  5:135077/149‑1 (MQ=255)                                                                                                        gTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCtc                                         >  8:113136/1‑148 (MQ=255)                                                                                                           cttcAAGATAGGGGCTAAATGTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGGACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAg                                                     <  6:27753/131‑1 (MQ=255)                                                                                                           cTACACGAAAGGGGCAAACTCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCCCACACCGTGAGT‑AAGTGGTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTaa                                     <  6:249896/149‑1 (MQ=255)                                                                                                           cTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTaaa                                                                                                              <  8:273565/77‑1 (MQ=255)                                                                                                           cTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTaaa                                                                                                              >  7:273565/1‑77 (MQ=255)                                                                                                           cTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGt                                       <  2:209181/148‑1 (MQ=255)                                                                                                           cTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGt                                       >  8:271171/1‑148 (MQ=255)                                                                                                           cTACAAGAAAGGGGCAAAATCTGAATTTTTTAGCCGTTAGGAGTAACGGCATGTAACGCGCACACACCGTGAGTAAACTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTAAATCAAg                                                     >  5:27753/1‑134 (MQ=255)                                                                                                               aaGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAgg                                                    >  1:113647/1‑131 (MQ=255)                                                                                                               aaGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAgg                                                    <  2:113647/131‑1 (MQ=255)                                                                                                                aGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGcc                                           <  5:13381/139‑1 (MQ=255)                                                                                                                aGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGcc                                           >  6:13381/1‑139 (MQ=255)                                                                                                                      gggCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTCGTAACTATgcgcgc                           <  7:113136/149‑1 (MQ=255)                                                                                                                        gCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATgcgc                             >  3:66592/1‑145 (MQ=255)                                                                                                                        gCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATgcgc                             <  4:66592/145‑1 (MQ=255)                                                                                                                         cAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTc                        <  8:290066/149‑1 (MQ=255)                                                                                                                             aTCTGATTTTTTTAGCCGTTAGGCGTAAGGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATgcgc                             >  1:18630/1‑140 (MQ=255)                                                                                                                             aTCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATgcgc                             <  2:18630/140‑1 (MQ=255)                                                                                                                                        ttAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGc          <  1:70215/148‑1 (MQ=255)                                                                                                                                         atgCCGTTACGCCTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCAt        >  3:144979/3‑149 (MQ=255)                                                                                                                                         atgCCGTTACGCCTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCAt        >  3:278455/3‑149 (MQ=255)                                                                                                                                           gCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCATTc      <  7:271171/149‑1 (MQ=255)                                                                                                                                           gCCGTTACGCCTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCATTc      >  1:212499/1‑149 (MQ=255)                                                                                                                                              gTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCa         >  6:174470/1‑143 (MQ=255)                                                                                                                                              gTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCa         <  5:174470/143‑1 (MQ=255)                                                                                                                                               ttAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCATTCTGaa  <  8:250738/149‑1 (MQ=255)                                                                                                                                               ttACGCCTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCATTCTGaa  <  2:212499/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                               ATTTGAGTCGGCCTGTCAAAGCCGGTCGTTGAATGGCAACGACCAAGCGAGACTAGTGGCCAACT‑CGGAACTGCACAATGGGCGACAGTATCTTTGGGAAATGTCCTACAAGAAAGGGGCAAAATCTGATTTTTTTAGCCGTTAGGCGTAACGGCATGTAACGCGCACACACCGTGAGTAAAGTGTTTTTCTGCGTGGAGGCACGCCTGGCGGTGCATAGCGCATAACGCTCAATCAAGGGCACTCGCCTTGTAACTATGCGCGCGTCTTGAGTCGCTGTGCATTCTGAA  >  NC_002947/5149036‑5149325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑