Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,154,168 G→T 7.6% F15L (TTC→TTA)  PP_4537 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,154,1680GT 7.6% 192.0 / 4.5 62F15L (TTC→TTA) PP_4537membrane protein
Reads supporting (aligned to +/- strand):  ref base G (27/29);  new base T (2/3);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CCAATCAGCAGCATGGAACGCATGAAGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGTTCAACCATGAGGAATGTAGGGGCTGTACCGGCCTCTTCGCGGGCACGCCC  >  NC_002947/5154022‑5154315
                                                                                                                                                   |                                                                                                                                                   
ccAATCAGCAGCATGGAACGCATGAAGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTTaa                                                                                                                                                   <  5:288887/149‑1 (MQ=255)
 cAATCAGCAGCATGGAACGCATGAAGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAg                                                                                                                                                  <  8:151107/149‑1 (MQ=255)
  aaTCAGCAGCATGGAACGCATGAAGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAgg                                                                                                                                                 <  7:190600/149‑1 (MQ=255)
           caTGGAACGCATGAAGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCt                                                                                                                                        <  7:283349/149‑1 (MQ=255)
             tGGAACGCATGAAGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTg                                                                                                                                       >  5:317347/1‑148 (MQ=255)
              ggAACGCATGAAGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGa                                                                                                                                     >  4:41405/1‑149 (MQ=255)
               gAACGCATGAAGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGa                                                                                                                                     <  4:88062/148‑1 (MQ=255)
                 aCGCATGAAGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCa                                                                                                                                  <  7:89370/149‑1 (MQ=255)
                   gCATGAAGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAgc                                                                                                                                <  8:74331/149‑1 (MQ=255)
                     aTGAAGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAgcgc                                                                                                                              >  1:152580/1‑149 (MQ=255)
                     aTGAAGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAgcgc                                                                                                                              >  1:245860/1‑149 (MQ=255)
                     aTGAAGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAgcgc                                                                                                                              <  2:245860/149‑1 (MQ=255)
                       gAAGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGcga                                                                                                                            >  7:278718/1‑149 (MQ=255)
                         aGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGcgac                                                                                                                           >  7:82733/1‑148 (MQ=255)
                          gCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGcgacga                                                                                                                         <  2:141053/149‑1 (MQ=255)
                              ggCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTAt                                                                                                                     <  2:152580/149‑1 (MQ=255)
                                 tccaCCTGGCCCCAGCGATTCAGCGGCAGGAACACCAGGCCTGCCAGCAGCACCCTGGC‑GACCACTGCGAAATAGCTGTCGACGTGCCCGCCCACGTACTCGCCGATCAAGCTCAAGGAAAACGCCTCGATCAGCTCCACGAatataa                                                                                                                   >  4:113967/4‑146 (MQ=255)
                                    aCCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATa                                                                                                               <  6:317347/149‑1 (MQ=255)
                                       tGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGcc                                                                                                            >  7:214117/1‑149 (MQ=255)
                                                           cAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAAt                                                                                        <  3:41405/149‑1 (MQ=255)
                                                            aGGAACACCAGGCCGGCCAGCAGCACCCTGGCGGAGCACTCCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGaa                                                                                         >  1:202791/1‑148 (MQ=255)
                                                              gAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCaa                                                                                     <  8:214117/149‑1 (MQ=255)
                                                                  acCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGcc                                                                                 >  5:123133/1‑149 (MQ=255)
                                                                    cacgccgggcATCAGCACCCTGGG‑GTTGACTGCGTAATATCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAATACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCcgc                                                                               <  7:200199/146‑1 (MQ=255)
                                                                    cAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCa                                                                                                          >  8:45995/1‑122 (MQ=255)
                                                                    cAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCa                                                                                                          <  7:45995/122‑1 (MQ=255)
                                                                                  gcaCCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCTGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGtt                                                                 <  8:278718/149‑1 (MQ=255)
                                                                                  gcaCCCTGGC‑GAGAACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGACGCGACCTTAGCGGGtt                                                                 >  2:13285/1‑149 (MQ=255)
                                                                                    aCCCTGGC‑GAGCACTGCGAAATAGCTGTCGCCGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGGTGc                                                               <  8:118572/149‑1 (MQ=255)
                                                                                    aCCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTg                                                                >  6:9346/1‑148 (MQ=255)
                                                                                     cccTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTa                                                                        <  1:132999/139‑1 (MQ=255)
                                                                                     cccTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTa                                                                        >  2:132999/1‑139 (MQ=255)
                                                                                      ccTGGC‑GAGCACTTCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTTAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAg                                                             >  3:204977/1‑149 (MQ=255)
                                                                                      ccTGGC‑GAGCACTTCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTTAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAg                                                             >  3:200989/1‑149 (MQ=255)
                                                                                      ccTGGC‑GAGCACTGCGAAATAGCTGTCGACTTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAg                                                             <  3:178192/149‑1 (MQ=255)
                                                                                        tGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGc                                                            >  1:9576/1‑148 (MQ=255)
                                                                                          gC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGcga                                                                                                                            >  1:137881/1‑82 (MQ=255)
                                                                                          gC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGcga                                                                                                                            <  2:137881/82‑1 (MQ=255)
                                                                                          gC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGaa                                                         <  7:268134/149‑1 (MQ=255)
                                                                                                 aCTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAg                                                                                    <  7:285864/116‑1 (MQ=255)
                                                                                                 aCTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAg                                                                                    >  8:285864/1‑116 (MQ=255)
                                                                                                 aCTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGtt                                                   >  2:247564/1‑149 (MQ=255)
                                                                                                   tGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGTTCa                                                 <  3:113967/149‑1 (MQ=255)
                                                                                                          tAGCTGTCGACGTGCCCGACCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGTTCAACCATGa                                          <  5:161534/149‑1 (MQ=255)
                                                                                                              tGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGTTCAACCATGAGGaa                                      >  5:100672/1‑149 (MQ=255)
                                                                                                                tCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGTTCAACCATGAGGaa                                      <  5:9346/147‑1 (MQ=255)
                                                                                                                 cGACCTCCCCGGCCAGGTAATATCCGATCAAACTGAAGGAAAACTACTAGATCAGAGCGACGAATATCAGATAGCCCATGGTTGCCTCTCGTGAATAAAGGCCGCAACATTAGCGTCTTGAAGCGAAAGAGTTCAACCATGAGGAATg                                    >  8:298818/1‑148 (MQ=255)
                                                                                                                  gACGTGCCCGGCCAGGTACTCGCCGATCAAGCTTAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGTTCAACCATGAGGAATGt                                   <  4:204977/148‑1 (MQ=255)
                                                                                                                  gACGTGCCCGGCCAGGTACTCGCCGATCAAGCTTAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGTTCAACCATGAGGAATGt                                   <  4:200989/148‑1 (MQ=255)
                                                                                                                     gTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGt                                                                                            >  4:42569/1‑88 (MQ=255)
                                                                                                                     gTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGt                                                                                            <  3:42569/88‑1 (MQ=255)
                                                                                                                     gTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGTTCAACCATGAGGAATGTAggg                               <  1:247564/149‑1 (MQ=255)
                                                                                                                         ccGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGtt                                                                 >  4:144228/1‑111 (MQ=255)
                                                                                                                         ccGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGtt                                                                 <  3:144228/111‑1 (MQ=255)
                                                                                                                                     tCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGTTCAACCATGAGGAATGTAGGGGCTGTACCGGCCTCtt               >  5:151571/1‑149 (MQ=255)
                                                                                                                                       gCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGTTCAACCATGAGGAATGTAGGGGCTGTACCGGCCTCTTc              <  2:9576/148‑1 (MQ=255)
                                                                                                                                               aGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGTTCAACCATGAGGAATGTa                                  >  8:169544/1‑120 (MQ=255)
                                                                                                                                                gCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCGATGGGTGCCTCTCGTGAATCAAGGCCGCGAGCTTAGCGGGTTGCAGCGAAGGAGTTCAAGCATGAGGAATGTAGGGGCTGTACCGGGCTCTTCGCGGGCACGc    >  6:167992/1‑149 (MQ=255)
                                                                                                                                                gCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGTTCAACCATGAGGAATGTAGGGGCTGTACCGGCCTCTTCGCGGGCACGc    >  3:83113/1‑149 (MQ=255)
                                                                                                                                                gCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGTTCAACCATGAGGAATGTAGGGGCTGTACCGGCCTCTTCGCGGGCACGc    >  2:317925/1‑149 (MQ=255)
                                                                                                                                                  tGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGTTCAACCATGAGGAATGTAGGGGCTGTACCGGCCTCTTCGCGGGCACGccc  <  1:13285/149‑1 (MQ=255)
                                                                                                                                                  tGAAGGAAAACGCCTGGATCAGCGCGACGATTATCACATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCTGGTTGCAGCGAATGAGTTCAACCATGAGGAATGTAGGGGCTGTACCGGCCTCTTCGCGGGCACGccc  >  5:259544/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCAATCAGCAGCATGGAACGCATGAAGCGTGGCTCGACCTGGCGCCAGCGGGTCAGCGGCAGGAACACCAGGCCGGCCAGCAGCACCCTGGC‑GAGCACTGCGAAATAGCTGTCGACGTGCCCGGCCAGGTACTCGCCGATCAAGCTGAAGGAAAACGCCTGGATCAGCGCGACGATTATCAGATAGCCCATGGTTGCCTCTCGTGAATCAAGGCCGCGACCTTAGCGGGTTGCAGCGAATGAGTTCAACCATGAGGAATGTAGGGGCTGTACCGGCCTCTTCGCGGGCACGCCC  >  NC_002947/5154022‑5154315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑