Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 468,461 C→T 9.4% A832A (GCG→GCA)  PP_0386 ← sensory box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947468,4610CT 9.4% 146.2 / 4.5 53A832A (GCG→GCA) PP_0386sensory box protein
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base T (3/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

CGGTGTCAACGTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAACCGCCACGAAGTGGG  >  NC_002947/468313‑468606
                                                                                                                                                     |                                                                                                                                                 
cGGTGTCAACGTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTgc                                                                                                                                                   <  5:290520/149‑1 (MQ=255)
 ggTGTCAACGTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTgcg                                                                                                                                                  >  2:49943/1‑149 (MQ=255)
  gtgtCAACGTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTgcg                                                                                                                                                  >  8:39203/1‑148 (MQ=255)
        aCGTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGt                                                                                                                                           <  5:116145/149‑1 (MQ=255)
        aCGTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGt                                                                                                                                           <  3:19774/149‑1 (MQ=255)
           tCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCCGCCCTGGCGGTTGGACACCTGCGCACAGGTGa                                                                                                                                         <  1:42366/149‑1 (MQ=255)
           tCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGTTTGGACACCTGCGCACAGGTGAg                                                                                                                                        >  8:111274/1‑149 (MQ=255)
                   cTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGCAGGTCGCCGCCCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCt                                                                                                                                >  7:240260/1‑149 (MQ=255)
                      ctctTTCAGGCGCTGGGCGATGCCCTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTg                                                                                                                               >  6:225553/1‑147 (MQ=255)
                        ctTTCAGGCGCTGGGCGATGCCCTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGc                                                                                                                           <  6:156786/149‑1 (MQ=255)
                              ggCGCTGGGCGATGCCCTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGttt                                                                                                                     >  2:316857/1‑149 (MQ=255)
                                cgcTGGGCGATGCCCTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTc                                                                                                                   <  1:49943/149‑1 (MQ=255)
                                     ggCGATGCCCTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACa                                                                                                              <  1:316857/149‑1 (MQ=255)
                                         aTGCCCTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGt                                                                                                          <  3:276804/149‑1 (MQ=255)
                                             ccTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGa                                                                                                      >  7:61286/1‑149 (MQ=255)
                                               ttGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGt                                                                                                                                           <  3:104075/110‑1 (MQ=255)
                                               ttGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGt                                                                                                                                           >  4:104075/1‑110 (MQ=255)
                                                    cagcagGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGAACAGGTGAGTACGCCCTGGAGCCGCTTTTCGAACAAGGTGCGATTggt                                                                                                 >  8:200475/1‑146 (MQ=255)
                                                      gcagGTCGCAGACCGGGTGGCCAAGGCTGTCGTTTATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCTCTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAgc                                                                                             <  7:200475/149‑2 (MQ=255)
                                                                gACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCg                                                                                   >  2:77574/1‑149 (MQ=255)
                                                                 aCCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGt                                                                                                          <  7:197137/125‑1 (MQ=255)
                                                                 aCCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGt                                                                                                          >  8:197137/1‑125 (MQ=255)
                                                                          ccAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCgg                                                                                  <  1:38517/140‑1 (MQ=255)
                                                                          ccAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCgg                                                                                  >  2:38517/1‑140 (MQ=255)
                                                                                   tCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGa                                                                  <  4:212797/147‑1 (MQ=255)
                                                                                   tCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATa                                                                <  7:238560/149‑1 (MQ=255)
                                                                                      ttGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCGCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTc                                                             <  5:225553/149‑1 (MQ=255)
                                                                                      ttGATGTGCGTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTc                                                             <  8:240260/149‑1 (MQ=255)
                                                                                        gATGTGCTTGAAGCGGTCGAGGTCCAGGAACCGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTc                                                             >  5:81012/1‑147 (MQ=255)
                                                                                        gATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCg                                                            <  7:39203/148‑1 (MQ=255)
                                                                                              cTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTgg                                                     >  6:3646/1‑149 (MQ=255)
                                                                                                 gAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCt                                                  >  5:85515/1‑149 (MQ=255)
                                                                                                 gAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCt                                                  >  2:139220/1‑149 (MQ=255)
                                                                                                   aGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTgg                                                >  8:68095/1‑149 (MQ=255)
                                                                                                        tCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTg                                           >  1:254480/1‑149 (MQ=255)
                                                                                                             gTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGa                                      <  2:254480/149‑1 (MQ=255)
                                                                                                               ccAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATg                                    >  6:310682/1‑149 (MQ=255)
                                                                                                                cAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTc                                                                               >  1:200741/1‑105 (MQ=255)
                                                                                                                cAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTc                                                                               <  2:200741/105‑1 (MQ=255)
                                                                                                                                  ccTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCaaaa                  <  1:77574/149‑1 (MQ=255)
                                                                                                                                      gCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCt                        <  7:37590/139‑1 (MQ=255)
                                                                                                                                      gCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCt                        >  8:37590/1‑139 (MQ=255)
                                                                                                                                      gCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAAcc               <  6:85515/148‑1 (MQ=255)
                                                                                                                                        ggTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGga                               <  4:221454/130‑1 (MQ=255)
                                                                                                                                        ggTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGga                               >  3:221454/1‑130 (MQ=255)
                                                                                                                                        ggTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAACCGcc            >  5:209104/1‑149 (MQ=255)
                                                                                                                                           tactcacCTGTGCGCAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGga                               >  6:263929/5‑127 (MQ=255)
                                                                                                                                           tactcacCTGTGCGCAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGga                               <  5:263929/123‑1 (MQ=255)
                                                                                                                                            actcacCTGTGCGCAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTgg                                                <  1:96552/106‑1 (MQ=255)
                                                                                                                                            actcacCTGTGCGCAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTgg                                                >  2:96552/4‑109 (MQ=255)
                                                                                                                                              acacCTGCGCCCAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGGCGAGTTTGGCCTGGGCGTGCtt                                        <  1:265612/115‑1 (MQ=255)
                                                                                                                                              acacCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGGGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCtg                                        >  2:265612/1‑114 (MQ=255)
                                                                                                                                                acCTGTGCGCAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAACCGCCACGAAGTg    >  8:41930/1‑149 (MQ=255)
                                                                                                                                                acCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAACCGCCACGAAGTg    >  8:117169/1‑149 (MQ=255)
                                                                                                                                                  cTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAACCGCCACGAAGTggg  >  6:1489/1‑149 (MQ=255)
                                                                                                                                                  cTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGGCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAACCGCCACGAAGTggg  <  8:61286/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CGGTGTCAACGTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGCAGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTG‑CGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAACCGCCACGAAGTGGG  >  NC_002947/468313‑468606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑