Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,239,884	G→A	8.5%	A144V (GCG→GTG)	ldh ←	leucine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,239,884	0	G	A	8.5%	159.9 / 4.7	60	A144V (GCG→GTG)	ldh	leucine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (25/28);  new base A (2/3);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.94e-01

AGCTCCGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCC  >  NC_002947/5239737‑5240030                                                                                                                                                     |                                                                                                                                                    agcTCCGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATcgccgc                                                                                                                                                    <  2:105583/149‑1 (MQ=255)      cgcgCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCTCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCAtgtg                                                                                                                                               <  2:286020/149‑1 (MQ=255)       gcgcCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTgg                                                                                                                                              <  8:98562/149‑1 (MQ=255)           ccACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGAt                                                                                                                                          >  2:297515/1‑149 (MQ=255)           ccACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACACCCATCGCCACATGCGGCGAt                                                                                                                                          <  4:159769/149‑1 (MQ=255)            cACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCC‑CCAGCCAGTAAGGGGCAAGCAGCCCAGGCCGAACGAGGTTGTGGGCAGGATTGCCC‑CGAACACCCCCATGCCCCCATGTTGCGAAgg                                                                                                                                        >  2:74349/1‑149 (MQ=255)               cGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCACATGCGGCGATgggg                                                                                                                                      <  4:200874/149‑2 (MQ=255)                   tGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGATGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGcc                                                                                                                                  <  1:87294/149‑1 (MQ=255)                           cTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGTAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGGGGCGATGGGTCGCCCGATGCAg                                                                                                                          <  2:112178/149‑1 (MQ=255)                            tCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAgt                                                                                                                         >  8:110181/1‑149 (MQ=255)                             ccGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAgtg                                                                                                                        >  5:265770/1‑149 (MQ=255)                              cGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAgt                                                                                                                         <  4:191538/147‑1 (MQ=255)                                   gggCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTg                                                                                                                  <  2:51760/149‑1 (MQ=255)                                           ccAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACg                                                                                                            >  1:129915/1‑147 (MQ=255)                                            cAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAg                                                                                                         >  7:223381/1‑149 (MQ=255)                                             aaCATTGCCCAACCCCTGGACCGGTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAgg                                                                                                        <  7:110181/149‑1 (MQ=255)                                             aaCATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAgg                                                                                                        <  8:223381/149‑1 (MQ=255)                                                aTTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCg                                                                                                     >  2:79699/1‑149 (MQ=255)                                                     ccAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGACCACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTg                                                                                                   >  3:92339/1‑146 (MQ=255)                                                     ccAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTg                                                                                                   <  4:92339/146‑1 (MQ=255)                                                     ccAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGA‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtg                                                                                                >  7:199823/1‑149 (MQ=255)                                                     ccAACCCCAGGACCGCTACCCGCAAGCCTCTCCGGTCATCGCTGCCCAGCCGGACCGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtg                                                                                                <  6:153212/149‑1 (MQ=255)                                                       aaCCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtgtg                                                                                              >  4:115632/1‑149 (MQ=255)                                                          cccTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCACATGCGGCGATgggg                                                                                                                                      >  3:157211/1‑105 (MQ=255)                                                          cccTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCACATGCGGCGATgggg                                                                                                                                      <  4:157211/106‑2 (MQ=255)                                                            cTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCaa                                                                                         >  7:67029/1‑149 (MQ=255)                                                            cTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCaa                                                                                         >  6:239054/1‑149 (MQ=255)                                                               gACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATg                                                                                       <  5:239054/148‑1 (MQ=255)                                                                  cGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGt                                                                                   <  6:265770/149‑1 (MQ=255)                                                                       cccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATg                                                                             >  7:286752/1‑149 (MQ=255)                                                                             aGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAg                                                                       <  1:79699/149‑1 (MQ=255)                                                                              gCCTCTCAGGTCATCGCTGCCCAGCCGGAAAGAGGTTGT‑GGCACGTATGCCAGCGAACACACCCATCGCCGCATGAGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTg                                                                                                   >  3:19859/1‑121 (MQ=255)                                                                                 tctcAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTc                                                                    >  4:178592/1‑148 (MQ=255)                                                                                          aTCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCAc                                                          >  1:205371/1‑149 (MQ=255)                                                                                           tCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTTGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACt                                                         <  1:74349/149‑1 (MQ=255)                                                                                           tCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTACTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACt                                                         <  1:297515/149‑1 (MQ=255)                                                                                             gCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGt                                                       <  8:286752/149‑1 (MQ=255)                                                                                             gCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGt                                                       >  7:270358/1‑149 (MQ=255)                                                                                                   cAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACgg                                                 <  2:205371/149‑1 (MQ=255)                                                                                                     gCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGc                                                >  4:86585/1‑148 (MQ=255)                                                                                                      ccGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCga                                              >  3:71473/1‑149 (MQ=255)                                                                                                       cGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCgat                                             <  3:86585/149‑1 (MQ=255)                                                                                                         gAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCgatga                                           >  2:249409/1‑149 (MQ=255)                                                                                                          aaCGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCgatgat                                          <  8:270358/149‑1 (MQ=255)                                                                                                             gAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGaa                                       <  3:115632/149‑1 (MQ=255)                                                                                                                         aCGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCCTGc                             >  5:76657/1‑148 (MQ=255)                                                                                                                         aCGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCa                            <  4:71473/149‑1 (MQ=255)                                                                                                                          ggTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCTGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCaa                           <  2:129915/148‑1 (MQ=255)                                                                                                                           gTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAg                          <  3:178592/149‑1 (MQ=255)                                                                                                                           gTATGCACTCGAACACCCCAATCGCCGAATGTGGCGATTGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCTAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAg                          <  8:199823/149‑1 (MQ=255)                                                                                                                              tGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTa                       <  8:67029/149‑1 (MQ=255)                                                                                                                                 ccGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAg                          <  2:276163/143‑1 (MQ=255)                                                                                                                                 ccGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAg                          >  1:276163/1‑143 (MQ=255)                                                                                                                                  cgcgTACACGCCCTTCGCCTCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTGC‑ATGTCCAATGTCGAGGTGCCACTGTCCACGGCgat                                             <  7:154685/123‑1 (MQ=255)                                                                                                                                  cgcgAACACCCCCATCGCCGCATCTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTCCTGTGCGAAATGCAGTCC‑ATCTCCAAGGTCGAGGTGCCACTCTCCACGGCgat                                             >  8:154685/1‑123 (MQ=255)                                                                                                                                         acCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCg            >  6:279219/1‑149 (MQ=255)                                                                                                                                            cccATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCgatg                                            >  4:19558/1‑114 (MQ=255)                                                                                                                                            cccATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCgatg                                            <  3:19558/114‑1 (MQ=255)                                                                                                                                             ccATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCAATGTCCAAGGTCGAGGTGCCACTGTCCACGGCgatga                                           <  1:52039/115‑1 (MQ=255)                                                                                                                                             ccATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCgatga                                           >  2:52039/1‑114 (MQ=255)                                                                                                                                              cATCGCCACATGCGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCa        >  5:163390/1‑148 (MQ=255)                                                                                                                                                   ccgcATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGTGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGcc  >  5:238984/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AGCTCCGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGT‑GGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCC‑ATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCC  >  NC_002947/5239737‑5240030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑