Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,257,696 G→C 10.0% P36A (CCG→GCG)  PP_4634 ← alpha/beta family hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,257,6960GC10.0% 138.0 / 7.3 50P36A (CCG→GCG) PP_4634alpha/beta family hydrolase
Reads supporting (aligned to +/- strand):  ref base G (20/25);  new base C (3/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

TGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATT  >  NC_002947/5257548‑5257842
                                                                                                                                                    |                                                                                                                                                  
tGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCgg                                                                                                                                                    >  4:249413/1‑149 (MQ=255)
        aCGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTggg                                                                                                                                            <  6:39411/149‑1 (MQ=255)
         cGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTgggg                                                                                                                                           <  5:297114/149‑1 (MQ=255)
         cGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTgggg                                                                                                                                           <  8:286245/149‑1 (MQ=255)
                  gccggccgGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTg                                                                                                                                  <  2:314450/149‑1 (MQ=255)
                   cctgcctgGATTCGCCGATGCCACGGGAGTAGTAGGTCAGCACATCTCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGTCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTgg                                                                                                                                 <  6:22666/146‑1 (MQ=255)
                        cggGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTg                                                                                                                            <  3:249413/149‑1 (MQ=255)
                        cggGATTCGCCGATGCCACGGTAGTAGTAGGTCAGCACATAGCAGCCGTGGGCGCACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGAGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTg                                                                                                                            >  3:284566/1‑149 (MQ=255)
                             ttCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCgg                                                                                                                                                    <  4:198563/120‑1 (MQ=255)
                             ttCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCgg                                                                                                                                                    >  3:198563/1‑120 (MQ=255)
                                gCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCcagc                                                                                                                    <  5:53366/149‑1 (MQ=255)
                                    aTGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGaa                                                                                                                <  4:151128/149‑1 (MQ=255)
                                                     ggTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTAt                                                                                                <  4:108328/148‑1 (MQ=255)
                                                           cacaTCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCgg                                                                                         >  2:180562/1‑149 (MQ=255)
                                                           cacaTCGCAGCCGTGGGCGAACAGGTAGTAGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCgg                                                                                         >  2:44130/1‑149 (MQ=255)
                                                                  cAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCggg                                                                                  <  7:63405/149‑1 (MQ=255)
                                                                  cAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCggg                                                                                  >  7:110559/1‑149 (MQ=255)
                                                                     ccGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTg                                                                               >  7:137973/1‑149 (MQ=255)
                                                                       gTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGc                                                                             >  4:165178/1‑149 (MQ=255)
                                                                                 aGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTggg                                                                                                                                >  7:233406/1‑88 (MQ=255)
                                                                                 aGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTggg                                                                                                                                <  8:233406/88‑1 (MQ=255)
                                                                                 aGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGAAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATgggg                                                                    >  2:304889/1‑148 (MQ=255)
                                                                                  ggTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGAAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTa                                                                  <  1:304889/149‑1 (MQ=255)
                                                                                   gTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGATATGGGGTAg                                                                 >  6:62674/1‑149 (MQ=255)
                                                                                       tCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCgg                                                              <  2:241299/148‑1 (MQ=255)
                                                                                         ggCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATgggg                                                                    <  1:54708/140‑1 (MQ=255)
                                                                                         ggCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATgggg                                                                    >  2:54708/1‑140 (MQ=255)
                                                                                          gCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCg                                                                                          <  5:54299/117‑1 (MQ=255)
                                                                                          gCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCg                                                                                          >  6:54299/1‑117 (MQ=255)
                                                                                             aaGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGAt                                                       <  8:137973/149‑1 (MQ=255)
                                                                                              aGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGCGTGGCGAAATGGGGTAGCGGGTTGGATg                                                      <  1:180562/149‑1 (MQ=255)
                                                                                               gCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGAt                                                       >  6:196324/1‑147 (MQ=255)
                                                                                                            cGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAgg                                                   >  7:201413/1‑138 (MQ=255)
                                                                                                            cGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAgg                                                   <  8:201413/138‑1 (MQ=255)
                                                                                                                 gCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATg                                   >  2:306735/1‑149 (MQ=255)
                                                                                                                             ggCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgttgttgt                       >  6:192127/1‑149 (MQ=255)
                                                                                                                               cAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTgc                     <  3:165178/149‑1 (MQ=255)
                                                                                                                                aGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTgcg                    >  1:165191/1‑149 (MQ=255)
                                                                                                                                  ccaacctgatCCCCAGCGCCCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTgc                     >  5:168364/7‑146 (MQ=255)
                                                                                                                                  ccaacctgatCCCCAGCGCCCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTgc                     <  6:168364/140‑1 (MQ=255)
                                                                                                                                  cGTTGTTGATGAGCAGCGGGCGCTGGGGATCAGGTGGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGGGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGcc                  <  8:110559/149‑1 (MQ=255)
                                                                                                                                     acctgatCCCCAGCGCCCGCTGGTGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgtt                            <  1:146616/133‑1 (MQ=255)
                                                                                                                                     acctgatCCCCAGCGCCCGCTGGTGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgtt                            >  2:146616/4‑136 (MQ=255)
                                                                                                                                         gatCCCCAGCGCCCGCTGGTGATCAGGATGGGCGTGGCGCCAGCGGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAAc             >  6:87737/1‑147 (MQ=255)
                                                                                                                                          atCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTa           <  1:306735/148‑1 (MQ=255)
                                                                                                                                          atCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAg          >  5:326671/1‑149 (MQ=255)
                                                                                                                                           tCACCAGCGGGCGTTGGGGATCAGGTTGGTGGTTGCTCCAGCAGAAGCCGCCGAGGCTGTATCCGTGGGGCGCGGGGTTGCGAAATGGGTTAGCGTGTTGGATGAGGCTACTCATTGCGCTTGTACTGTTGTTGTGCGCCAAAACTAGc         <  4:284566/149‑1 (MQ=255)
                                                                                                                                           tCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGtt                                                           >  7:66299/1‑99 (MQ=255)
                                                                                                                                           tCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGtt                                                           <  8:66299/99‑1 (MQ=255)
                                                                                                                                            cACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtg                              <  4:164522/127‑1 (MQ=255)
                                                                                                                                            cACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtg                              >  3:164522/1‑127 (MQ=255)
                                                                                                                                                  cGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGAtt  >  6:244107/1‑149 (MQ=255)
                                                                                                                                                  cGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGAtt  <  5:192127/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATT  >  NC_002947/5257548‑5257842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑