Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,266,831	T→G	8.6%	F23V (TTC→GTC)	PP_4642 →	cyclic diguanosine monophosphate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,266,831	0	T	G	8.6%	168.7 / 8.0	58	F23V (TTC→GTC)	PP_4642	cyclic diguanosine monophosphate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (26/27);  new base G (2/3);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.81e-01

TTGCTTCGTTCTAGAGCCTCTGCGGCCAGATTGCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGATTTCGACGCCATCATCGACCTGGACAA  >  NC_002947/5266684‑5266979                                                                                                                                                     |                                                                                                                                                      ttGCTTCGTTCTAGAGCCTCTGCGGCCAGATTGCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACtt                                                                                                                                                      >  7:17150/1‑149 (MQ=255)     ttCGTTCTAGAGCCTCTGCGGCCAGATTGCAGTACCTGATGCACAAGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAt                                                                                                                                                  >  8:271653/1‑149 (MQ=255)       cGTTCTAGAGCCTCTGCGGCCAGATTGCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAt                                                                                                                                                  <  7:7038/147‑1 (MQ=255)       cGTTCTAGAGCCTCTGCGGCCAGATTGCAGTACCTGATGCACAAGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAt                                                                                                                                                  <  7:271653/147‑1 (MQ=255)            tAGAGCCTCTGCGGCCAGATTGCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCa                                                                                                                                          >  1:309919/1‑149 (MQ=255)             agagCCTCTGCGGCCAGATTGCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCAc                                                                                                                                         >  7:98406/1‑149 (MQ=255)               agCCTCTGCGGCCAGATTGCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGAAGTCGAT‑Gcgttgaaa                                                                                                                                        <  4:145289/148‑8 (MQ=255)                     tGCGGCCAGATTGCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTgc                                                                                                                                 >  1:15477/1‑149 (MQ=255)                     tGCGGCCAGATTGCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTgc                                                                                                                                 >  2:133820/1‑149 (MQ=255)                      gCGGCCAGATTGCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACtt                                                                                                                                                      <  6:113874/128‑1 (MQ=255)                      gCGGCCAGATTGCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACtt                                                                                                                                                      >  5:113874/1‑128 (MQ=255)                       cGGCCAGATTGCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTgcgc                                                                                                                               <  3:130669/149‑1 (MQ=255)                        ggCCAGATTGCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCCGATGACCTATTCGAGGATCCATGAAATGATCGATC‑ATGACGAACGCAGCCGATTTCAACGCATCGACTTCGAT‑GCCCACAACGAGCTgcgc                                                                                                                               >  6:326796/1‑148 (MQ=255)                         gCCAGATTGCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAg                                                                                                                                     >  3:200816/1‑141 (MQ=255)                         gCCAGATTGCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAg                                                                                                                                     <  4:200816/141‑1 (MQ=255)                                 gCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTg                                                                                                                      >  5:72002/1‑148 (MQ=255)                                  cAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGa                                                                                                                     <  7:195505/148‑1 (MQ=255)                                        cTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTg                                                                                                              <  1:133820/149‑1 (MQ=255)                                           aTGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGc                                                                                                            <  8:181508/148‑1 (MQ=255)                                              cacaTGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTg                                                                                                         <  7:278940/148‑1 (MQ=255)                                               acaTGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATA‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGc                                                                                                        >  8:164432/1‑148 (MQ=255)                                                 aTGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCaa                                                                                                      >  2:139737/1‑148 (MQ=255)                                                     aaCTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATCGATAACGAACGCCCCCGATTTAAACGCTACGACTACTAT‑CCCCCACCCTACATCCGCCAGGGGATGAAACCATGGCCTGAAAAAct                                                                                                  >  1:253205/1‑149 (MQ=255)                                                       ctctAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAActgct                                                                                               <  1:183078/149‑1 (MQ=255)                                                        tctAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAActgctg                                                                                              >  6:181301/1‑149 (MQ=255)                                                             cTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCt                                                                                         >  8:49979/1‑149 (MQ=255)                                                                 aGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAg                                                                                                                                     >  7:40046/1‑101 (MQ=255)                                                                 aGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAg                                                                                                                                     <  8:40046/101‑1 (MQ=255)                                                                   tCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGATGGCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAAc                                                                                                   <  1:224294/134‑1 (MQ=255)                                                                       gATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCaaa                                                                               <  8:98406/149‑1 (MQ=255)                                                                       gATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCaaa                                                                               <  2:309919/149‑1 (MQ=255)                                                                                      ccTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTg                                                                         >  6:278255/1‑140 (MQ=255)                                                                                      ccTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTg                                                                         <  5:278255/140‑1 (MQ=255)                                                                                       cTATTCGAGTATCCATGCCATGAGCGATC‑ATGACGAACTCCGCCGCTTTCAACGCATCGACTTCGAT‑GGCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTTCTGGACCTTTCACTCAAAGGCTTTCTGATCGAAc                                                               <  5:326796/149‑1 (MQ=255)                                                                                         aTTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGc                                                             >  1:14815/1‑149 (MQ=255)                                                                                          ttCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCt                                                                                                                       <  8:133202/90‑1 (MQ=255)                                                                                             gAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAgggg                                                                                                                         >  8:292230/1‑85 (MQ=255)                                                                                             gAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAgggg                                                                                                                         <  7:292230/85‑1 (MQ=255)                                                                                             gAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCgg                                                         <  1:139737/149‑1 (MQ=255)                                                                                             gAGCATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTATCACTCAAAGGCTTGCTGATCGAACTCCCgg                                                         >  6:90249/1‑149 (MQ=255)                                                                                                    catgccatgAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTg                                                  <  2:15477/149‑1 (MQ=255)                                                                                                     atgccatgAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTgg                                                 <  7:164432/149‑1 (MQ=255)                                                                                                     atgccatgAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTCAAACGCTGGCCTGCAAAACTGCTGGACCATGCACTCAACGGCTTGCTGATCGAAAGCCCCGCACACTgc                                                 >  8:292312/1‑148 (MQ=255)                                                                                                             aGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACCCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGa                                         <  5:285565/149‑1 (MQ=255)                                                                                                                 aTC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCt                                      <  5:4314/148‑1 (MQ=255)                                                                                                                 aTC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCt                                      >  6:4314/1‑148 (MQ=255)                                                                                                                          gAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGccc                                                           <  7:134873/119‑1 (MQ=255)                                                                                                                          gAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGccc                                                           >  8:134873/1‑119 (MQ=255)                                                                                                                          gAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGa                             >  5:66225/1‑149 (MQ=255)                                                                                                                             cgccgccgcTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGAttt                          <  8:17150/149‑1 (MQ=255)                                                                                                                                 gccgcTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGACTGCAAAACTGCTGGACCTTTCACTCAATGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGATTTCGAc                      <  5:90249/149‑1 (MQ=255)                                                                                                                                         cAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGATTTCGACGCcatca               <  2:14815/148‑1 (MQ=255)                                                                                                                                          aaCGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGATTTCGACGCCatcatc             >  8:204429/1‑149 (MQ=255)                                                                                                                                           gggCATCGAAGTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGAttt                          >  5:285338/3‑135 (MQ=255)                                                                                                                                           gggCATCGAAGTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGAttt                          >  5:308123/3‑135 (MQ=255)                                                                                                                                           gggCATCGAAGTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGAttt                          <  6:308123/133‑1 (MQ=255)                                                                                                                                           gggCATCGAAGTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGAttt                          <  6:285338/133‑1 (MQ=255)                                                                                                                                           ccGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCGAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGATTTCGACGCCATCATCg            <  7:292312/148‑1 (MQ=255)                                                                                                                                                tCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGATTTCGACGCCATCATCGACCTg       >  2:17913/1‑149 (MQ=255)                                                                                                                                                tCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGATTTCGACGCCATCATCGACCTg       >  1:238591/1‑149 (MQ=255)                                                                                                                                                   aCTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCGAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGATTTCGACGCCATCATCGACCTGGa     >  8:53754/1‑148 (MQ=255)                                                                                                                                                     ttCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGATTTCGACGCCATCATCGACCTGGACaa  >  6:164565/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                      TTGCTTCGTTCTAGAGCCTCTGCGGCCAGATTGCAGTACCTGATGCACATGCAACTCTAACTATAGTCAGGATGACACCTGATGACCTATTCGAGGATCCATGCCATGAGCGATC‑ATGACGAACGCCGCCGCTTTCAACGCATCGACTTCGAT‑GCCCCCACCGAGCTGCGCCAGGGGCTGAAACGCTGGCCTGCAAAACTGCTGGACCTTTCACTCAAAGGCTTGCTGATCGAACGCCCGGCAGACTGGGATGCCGACCTGACTCAGGATTTCGACGCCATCATCGACCTGGACAA  >  NC_002947/5266684‑5266979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑